Surface-enhanced Raman scattering (SERS) spectroscopy in small molecule detection and application
Surface-enhanced Raman scattering (SERS) spectroscopy is an advanced analytical technique for bioanalysis. However, one of the key challenges that impedes SERS for actual biomolecule detection is due to two reasons: 1) the intrinsically low Raman activities of biomolecules and 2) the lack of interac...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2022
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| Online Access: | https://hdl.handle.net/10356/158977 |
| _version_ | 1811682314756816896 |
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| author | Kao, Ya-Chuan |
| author2 | Ling Xing Yi |
| author_facet | Ling Xing Yi Kao, Ya-Chuan |
| author_sort | Kao, Ya-Chuan |
| collection | NTU |
| description | Surface-enhanced Raman scattering (SERS) spectroscopy is an advanced analytical technique for bioanalysis. However, one of the key challenges that impedes SERS for actual biomolecule detection is due to two reasons: 1) the intrinsically low Raman activities of biomolecules and 2) the lack of interactions between the analyte and plasmonic nanoparticles. The objectives of my thesis is to address these limitations to achieve the practical applications using SERS spectroscopy via platform design and modification of the plasmonic nanoparticles surface chemistry. In chapter 2, we demonstrate a two-part strategy at the oil/water interface to control the single building block morphology over the orientational ordering of supercrystals by tuning surface ligands. In chapter 3 we utilize two types of Ag nanoparticle to form a superhydrophobic SERS detection platform for two urinary metabolites via capture-and confine strategy at the molecule-level. In chapter 4, we follow up the detection strategy from chapter 3 as a proof-of-concept diagnostic kit for spontaneous miscarriage by evaluating the ratio between 5β-pregnane-3α,20α-diol-3α-glucuronide (pregnane) and tetrahydrocortisone (THC). In chapter 5, we further exploit the confine-and-capture strategy for different structural variants analysis. The different binding sites and molecular states of the multiple analytes and probes enable the formation of diversified molecular complexes with different configurations, thus intensifying the specific differences in SERS fingerprints obtained by the various analytes. Lastly, I conclude my thesis with a summary for the different research work and provide an outlook for continuous study in the biomolecule detection and further applications. |
| first_indexed | 2024-10-01T03:54:53Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/158977 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:54:53Z |
| publishDate | 2022 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1589772023-02-28T23:38:08Z Surface-enhanced Raman scattering (SERS) spectroscopy in small molecule detection and application Kao, Ya-Chuan Ling Xing Yi School of Physical and Mathematical Sciences XYLing@ntu.edu.sg Science::Chemistry Surface-enhanced Raman scattering (SERS) spectroscopy is an advanced analytical technique for bioanalysis. However, one of the key challenges that impedes SERS for actual biomolecule detection is due to two reasons: 1) the intrinsically low Raman activities of biomolecules and 2) the lack of interactions between the analyte and plasmonic nanoparticles. The objectives of my thesis is to address these limitations to achieve the practical applications using SERS spectroscopy via platform design and modification of the plasmonic nanoparticles surface chemistry. In chapter 2, we demonstrate a two-part strategy at the oil/water interface to control the single building block morphology over the orientational ordering of supercrystals by tuning surface ligands. In chapter 3 we utilize two types of Ag nanoparticle to form a superhydrophobic SERS detection platform for two urinary metabolites via capture-and confine strategy at the molecule-level. In chapter 4, we follow up the detection strategy from chapter 3 as a proof-of-concept diagnostic kit for spontaneous miscarriage by evaluating the ratio between 5β-pregnane-3α,20α-diol-3α-glucuronide (pregnane) and tetrahydrocortisone (THC). In chapter 5, we further exploit the confine-and-capture strategy for different structural variants analysis. The different binding sites and molecular states of the multiple analytes and probes enable the formation of diversified molecular complexes with different configurations, thus intensifying the specific differences in SERS fingerprints obtained by the various analytes. Lastly, I conclude my thesis with a summary for the different research work and provide an outlook for continuous study in the biomolecule detection and further applications. Doctor of Philosophy 2022-06-06T03:07:09Z 2022-06-06T03:07:09Z 2021 Thesis-Doctor of Philosophy Kao, Y. (2021). Surface-enhanced Raman scattering (SERS) spectroscopy in small molecule detection and application. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/158977 https://hdl.handle.net/10356/158977 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Chemistry Kao, Ya-Chuan Surface-enhanced Raman scattering (SERS) spectroscopy in small molecule detection and application |
| title | Surface-enhanced Raman scattering (SERS) spectroscopy in small molecule detection and application |
| title_full | Surface-enhanced Raman scattering (SERS) spectroscopy in small molecule detection and application |
| title_fullStr | Surface-enhanced Raman scattering (SERS) spectroscopy in small molecule detection and application |
| title_full_unstemmed | Surface-enhanced Raman scattering (SERS) spectroscopy in small molecule detection and application |
| title_short | Surface-enhanced Raman scattering (SERS) spectroscopy in small molecule detection and application |
| title_sort | surface enhanced raman scattering sers spectroscopy in small molecule detection and application |
| topic | Science::Chemistry |
| url | https://hdl.handle.net/10356/158977 |
| work_keys_str_mv | AT kaoyachuan surfaceenhancedramanscatteringsersspectroscopyinsmallmoleculedetectionandapplication |