Self-assembled nanofibrous marine collagen matrix accelerates healing of full-thickness wounds
There is an urgent clinical need for wound dressings to treat skin injuries, particularly full-thickness wounds caused by acute and chronic wounds. Marine collagen has emerged as an attractive and safer alternative due to its biocompatibility, diversity, and sustainability. It has minimum risk of zo...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/159788 |
| _version_ | 1811692945911316480 |
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| author | Liu, Shao Qiong Wen, Feng Muthukumaran, Padmalosini Rakshit, Moumita Lau, Chau-Sang Yu, Na Suryani, Luvita Dong, Yibing Teoh, Swee-Hin |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Liu, Shao Qiong Wen, Feng Muthukumaran, Padmalosini Rakshit, Moumita Lau, Chau-Sang Yu, Na Suryani, Luvita Dong, Yibing Teoh, Swee-Hin |
| author_sort | Liu, Shao Qiong |
| collection | NTU |
| description | There is an urgent clinical need for wound dressings to treat skin injuries, particularly full-thickness wounds caused by acute and chronic wounds. Marine collagen has emerged as an attractive and safer alternative due to its biocompatibility, diversity, and sustainability. It has minimum risk of zoonotic diseases and less religious constraints as compared to mammalian collagen. In this study, we reported the development of a self-assembled nanofibrous barramundi (Lates calcarifer) collagen matrix (Nano-BCM), which showed good biocompatibility for full-thickness wound-healing applications. The collagen was extracted and purified from barramundi scales and skin. Thereafter, the physicochemical properties of collagen were systematically evaluated. The process to extract barramundi skin collagen (BC) gave an excellent 45% yield and superior purity (∼100%). More importantly, BC demonstrated structural integrity, native triple helix structure, and good thermal stability. BC demonstrated its efficacy in promoting human primary dermal fibroblast (HDF) and immortalized human keratinocytes (HaCaT) proliferation and migration. Nano-BCM has been prepared via self-assembly of collagen molecules in physiological conditions, which resembled the native extracellular matrix (ECM). The clinical therapeutic efficacy of the Nano-BCM was further evaluated in a full-thickness splinted skin wound mice model. In comparison to a clinically used wound dressing (DuoDerm), the Nano-BCM demonstrated significantly accelerated wound closure and re-epithelization. Moreover, Nano-BCM nanofibrous architecture and its ability to facilitate early inflammatory response significantly promoted angiogenesis and differentiated myofibroblast, leading to enhanced wound healing. Consequently, Nano-BCM demonstrates great potential as an economical and effective nonmammalian substitute to achieve skin regeneration. |
| first_indexed | 2024-10-01T06:43:51Z |
| format | Journal Article |
| id | ntu-10356/159788 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:43:51Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1597882022-07-02T12:29:09Z Self-assembled nanofibrous marine collagen matrix accelerates healing of full-thickness wounds Liu, Shao Qiong Wen, Feng Muthukumaran, Padmalosini Rakshit, Moumita Lau, Chau-Sang Yu, Na Suryani, Luvita Dong, Yibing Teoh, Swee-Hin School of Chemical and Biomedical Engineering Lee Kong Chian School of Medicine (LKCMedicine) Engineering::Bioengineering Marine Collagen Self-Assembly There is an urgent clinical need for wound dressings to treat skin injuries, particularly full-thickness wounds caused by acute and chronic wounds. Marine collagen has emerged as an attractive and safer alternative due to its biocompatibility, diversity, and sustainability. It has minimum risk of zoonotic diseases and less religious constraints as compared to mammalian collagen. In this study, we reported the development of a self-assembled nanofibrous barramundi (Lates calcarifer) collagen matrix (Nano-BCM), which showed good biocompatibility for full-thickness wound-healing applications. The collagen was extracted and purified from barramundi scales and skin. Thereafter, the physicochemical properties of collagen were systematically evaluated. The process to extract barramundi skin collagen (BC) gave an excellent 45% yield and superior purity (∼100%). More importantly, BC demonstrated structural integrity, native triple helix structure, and good thermal stability. BC demonstrated its efficacy in promoting human primary dermal fibroblast (HDF) and immortalized human keratinocytes (HaCaT) proliferation and migration. Nano-BCM has been prepared via self-assembly of collagen molecules in physiological conditions, which resembled the native extracellular matrix (ECM). The clinical therapeutic efficacy of the Nano-BCM was further evaluated in a full-thickness splinted skin wound mice model. In comparison to a clinically used wound dressing (DuoDerm), the Nano-BCM demonstrated significantly accelerated wound closure and re-epithelization. Moreover, Nano-BCM nanofibrous architecture and its ability to facilitate early inflammatory response significantly promoted angiogenesis and differentiated myofibroblast, leading to enhanced wound healing. Consequently, Nano-BCM demonstrates great potential as an economical and effective nonmammalian substitute to achieve skin regeneration. Agency for Science, Technology and Research (A*STAR) This work was funded by the Agency for Science, Technology and Research (A*STAR) RIE2020 Advanced Manufacturing and Engineering (AME) programmatic grant (A18A8b0059) Additive Manufacturing for Biological Materials (AMBM) project SP1.1. 2022-07-02T12:29:09Z 2022-07-02T12:29:09Z 2021 Journal Article Liu, S. Q., Wen, F., Muthukumaran, P., Rakshit, M., Lau, C., Yu, N., Suryani, L., Dong, Y. & Teoh, S. (2021). Self-assembled nanofibrous marine collagen matrix accelerates healing of full-thickness wounds. ACS Applied Bio Materials, 4(9), 7044-7058. https://dx.doi.org/10.1021/acsabm.1c00685 2576-6422 https://hdl.handle.net/10356/159788 10.1021/acsabm.1c00685 35006937 2-s2.0-85115777345 9 4 7044 7058 en A18A8b0059 ACS Applied Bio Materials © 2021 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering::Bioengineering Marine Collagen Self-Assembly Liu, Shao Qiong Wen, Feng Muthukumaran, Padmalosini Rakshit, Moumita Lau, Chau-Sang Yu, Na Suryani, Luvita Dong, Yibing Teoh, Swee-Hin Self-assembled nanofibrous marine collagen matrix accelerates healing of full-thickness wounds |
| title | Self-assembled nanofibrous marine collagen matrix accelerates healing of full-thickness wounds |
| title_full | Self-assembled nanofibrous marine collagen matrix accelerates healing of full-thickness wounds |
| title_fullStr | Self-assembled nanofibrous marine collagen matrix accelerates healing of full-thickness wounds |
| title_full_unstemmed | Self-assembled nanofibrous marine collagen matrix accelerates healing of full-thickness wounds |
| title_short | Self-assembled nanofibrous marine collagen matrix accelerates healing of full-thickness wounds |
| title_sort | self assembled nanofibrous marine collagen matrix accelerates healing of full thickness wounds |
| topic | Engineering::Bioengineering Marine Collagen Self-Assembly |
| url | https://hdl.handle.net/10356/159788 |
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