Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs
Autohydrolysis is used for producing xylan-derived oligosaccharides from lignocellulosic biomass. Although numerous studies report optimized autohydrolysis conditions for various plants, few of these studies correlate process parameters with the resulting structural properties to their impact on int...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/159898 |
| _version_ | 1811678299581054976 |
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| author | Zhao, Sainan Dien, Bruce S. Lindemann, Stephen R. Chen, Ming-Hsu |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Zhao, Sainan Dien, Bruce S. Lindemann, Stephen R. Chen, Ming-Hsu |
| author_sort | Zhao, Sainan |
| collection | NTU |
| description | Autohydrolysis is used for producing xylan-derived oligosaccharides from lignocellulosic biomass. Although numerous studies report optimized autohydrolysis conditions for various plants, few of these studies correlate process parameters with the resulting structural properties to their impact on intestinal bacterial communities. Thus, to further clarify these relationships, beechwood xylan (BWX)-derived substrates, processed under five conditions, were fermented in vitro by human gut microbiota. Autohydrolysis reduced the mean molecular size and substitutions of BWX. Distinct fermentation kinetics were observed with differing processing of BWX substrates, which correlated with impacts on community species evenness. The relative abundances of Bacteroides, Fusicatenibacter, Bifidobacterium, and Megasphaera within the fermentations varied with processing conditions. While the total short-chain fatty acid concentrations were the same among the treatments, processing conditions varied the extent of propionate and butyrate generation. Autolysis parameters may be an important tool for optimizing beneficial effects of xylan-derived fibers on human gut microbiota structure and function. |
| first_indexed | 2024-10-01T02:51:03Z |
| format | Journal Article |
| id | ntu-10356/159898 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:51:03Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1598982022-07-05T05:19:23Z Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs Zhao, Sainan Dien, Bruce S. Lindemann, Stephen R. Chen, Ming-Hsu School of Chemical and Biomedical Engineering Engineering::Bioengineering Autohydrolysis Pretreatment Autohydrolysis is used for producing xylan-derived oligosaccharides from lignocellulosic biomass. Although numerous studies report optimized autohydrolysis conditions for various plants, few of these studies correlate process parameters with the resulting structural properties to their impact on intestinal bacterial communities. Thus, to further clarify these relationships, beechwood xylan (BWX)-derived substrates, processed under five conditions, were fermented in vitro by human gut microbiota. Autohydrolysis reduced the mean molecular size and substitutions of BWX. Distinct fermentation kinetics were observed with differing processing of BWX substrates, which correlated with impacts on community species evenness. The relative abundances of Bacteroides, Fusicatenibacter, Bifidobacterium, and Megasphaera within the fermentations varied with processing conditions. While the total short-chain fatty acid concentrations were the same among the treatments, processing conditions varied the extent of propionate and butyrate generation. Autolysis parameters may be an important tool for optimizing beneficial effects of xylan-derived fibers on human gut microbiota structure and function. Nanyang Technological University This work was supported by the institutional fund provided by Nanyang Technological University. 2022-07-05T05:19:23Z 2022-07-05T05:19:23Z 2021 Journal Article Zhao, S., Dien, B. S., Lindemann, S. R. & Chen, M. (2021). Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs. Carbohydrate Polymers, 271, 118418-. https://dx.doi.org/10.1016/j.carbpol.2021.118418 0144-8617 https://hdl.handle.net/10356/159898 10.1016/j.carbpol.2021.118418 34364559 2-s2.0-85110024416 271 118418 en Carbohydrate Polymers © 2021 Elsevier Ltd. All rights reserved. |
| spellingShingle | Engineering::Bioengineering Autohydrolysis Pretreatment Zhao, Sainan Dien, Bruce S. Lindemann, Stephen R. Chen, Ming-Hsu Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs |
| title | Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs |
| title_full | Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs |
| title_fullStr | Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs |
| title_full_unstemmed | Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs |
| title_short | Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs |
| title_sort | controlling autohydrolysis conditions to produce xylan derived fibers that modulate gut microbiota responses and metabolic outputs |
| topic | Engineering::Bioengineering Autohydrolysis Pretreatment |
| url | https://hdl.handle.net/10356/159898 |
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