Enhanced catalytic activity induced by the nanostructuring effect in Pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid-β bioassay
In this work, we fabricated a novel origami paper-based analytical device (oPAD) assisted by the nanostructuring effect of in situ Pd decoration of Cu/Co-doped CeO2 (CuCo-CeO2-Pd) nanospheres, functionalized with their strongly enhanced electrocatalytic properties to realize an electrochemical and v...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/160112 |
| _version_ | 1826116186550566912 |
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| author | Cui, Kang Zhou, Chenxi Zhang, Bowei Zhang, Lina Liu, Yue Hao, Shiji Tang, Xiaohong Huang, Yizhong Yu, Jinghua |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Cui, Kang Zhou, Chenxi Zhang, Bowei Zhang, Lina Liu, Yue Hao, Shiji Tang, Xiaohong Huang, Yizhong Yu, Jinghua |
| author_sort | Cui, Kang |
| collection | NTU |
| description | In this work, we fabricated a novel origami paper-based analytical device (oPAD) assisted by the nanostructuring effect of in situ Pd decoration of Cu/Co-doped CeO2 (CuCo-CeO2-Pd) nanospheres, functionalized with their strongly enhanced electrocatalytic properties to realize an electrochemical and visual signal readout system in oPAD, for highly sensitive detection of amyloid-β (Aβ). The CuCo-CeO2-Pd nanospheres were introduced as an enhanced "signal transducer layer" on account of the electron transfer acceleration caused by catalyzing glucose to produce H2O2 for differential pulse voltammetry signal readout and further 3,3'5,5'-tetramethylbenzidine (TMB) oxidation for colorimetric analysis. Meanwhile, for achieving superior performance of the proposed oPAD, in situ growth of urchin-like gold nanoparticles (Au NPs) onto cellulose fibers was adopted to improve "the recognition layer" in favor of immobilizing antibodies for targeting Aβ through specific antigen-antibody interactions. Combined with the delicate design of oPAD, exhibiting actuation of the conversion procedure between hydrophobicity and hydrophilicity on paper tabs in the assay process, the oPAD successfully enabled sensitive diagnosis of Aβ in a linear range from 1.0 pM to 100 nM with a limit of detection of 0.05 pM (S/N = 3) for electrochemical detection, providing a reliable strategy for quantifying the Aβ protein in clinical applications. |
| first_indexed | 2024-10-01T04:07:14Z |
| format | Journal Article |
| id | ntu-10356/160112 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:07:14Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1601122022-07-13T01:14:54Z Enhanced catalytic activity induced by the nanostructuring effect in Pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid-β bioassay Cui, Kang Zhou, Chenxi Zhang, Bowei Zhang, Lina Liu, Yue Hao, Shiji Tang, Xiaohong Huang, Yizhong Yu, Jinghua School of Materials Science and Engineering School of Electrical and Electronic Engineering Engineering::Materials Amyloid-Beta Pd Decorating Doped Ceria In this work, we fabricated a novel origami paper-based analytical device (oPAD) assisted by the nanostructuring effect of in situ Pd decoration of Cu/Co-doped CeO2 (CuCo-CeO2-Pd) nanospheres, functionalized with their strongly enhanced electrocatalytic properties to realize an electrochemical and visual signal readout system in oPAD, for highly sensitive detection of amyloid-β (Aβ). The CuCo-CeO2-Pd nanospheres were introduced as an enhanced "signal transducer layer" on account of the electron transfer acceleration caused by catalyzing glucose to produce H2O2 for differential pulse voltammetry signal readout and further 3,3'5,5'-tetramethylbenzidine (TMB) oxidation for colorimetric analysis. Meanwhile, for achieving superior performance of the proposed oPAD, in situ growth of urchin-like gold nanoparticles (Au NPs) onto cellulose fibers was adopted to improve "the recognition layer" in favor of immobilizing antibodies for targeting Aβ through specific antigen-antibody interactions. Combined with the delicate design of oPAD, exhibiting actuation of the conversion procedure between hydrophobicity and hydrophilicity on paper tabs in the assay process, the oPAD successfully enabled sensitive diagnosis of Aβ in a linear range from 1.0 pM to 100 nM with a limit of detection of 0.05 pM (S/N = 3) for electrochemical detection, providing a reliable strategy for quantifying the Aβ protein in clinical applications. Ministry of Education (MOE) This work was financially supported by the Science and Technology Projects of University of Jinan (XKY2002), the Natural Science Foundation of Shandong Province (ZR2020MB057), the Singapore Ministry of Education (MOE AcRF Tier 1 RG176/16), the Taishan Scholars Program, the Case-by-Case Project for Top Outstanding Talents of Jinan, and the project of "20 Items of University" of Jinan (2018GXRC001). 2022-07-13T01:14:54Z 2022-07-13T01:14:54Z 2021 Journal Article Cui, K., Zhou, C., Zhang, B., Zhang, L., Liu, Y., Hao, S., Tang, X., Huang, Y. & Yu, J. (2021). Enhanced catalytic activity induced by the nanostructuring effect in Pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid-β bioassay. ACS Applied Materials and Interfaces, 13(29), 33937-33947. https://dx.doi.org/10.1021/acsami.1c09760 1944-8244 https://hdl.handle.net/10356/160112 10.1021/acsami.1c09760 34279896 2-s2.0-85111523426 29 13 33937 33947 en RG176/16 ACS Applied Materials and Interfaces © 2021 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering::Materials Amyloid-Beta Pd Decorating Doped Ceria Cui, Kang Zhou, Chenxi Zhang, Bowei Zhang, Lina Liu, Yue Hao, Shiji Tang, Xiaohong Huang, Yizhong Yu, Jinghua Enhanced catalytic activity induced by the nanostructuring effect in Pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid-β bioassay |
| title | Enhanced catalytic activity induced by the nanostructuring effect in Pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid-β bioassay |
| title_full | Enhanced catalytic activity induced by the nanostructuring effect in Pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid-β bioassay |
| title_fullStr | Enhanced catalytic activity induced by the nanostructuring effect in Pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid-β bioassay |
| title_full_unstemmed | Enhanced catalytic activity induced by the nanostructuring effect in Pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid-β bioassay |
| title_short | Enhanced catalytic activity induced by the nanostructuring effect in Pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid-β bioassay |
| title_sort | enhanced catalytic activity induced by the nanostructuring effect in pd decoration onto doped ceria enabling an origami paper analytical device for high performance of amyloid β bioassay |
| topic | Engineering::Materials Amyloid-Beta Pd Decorating Doped Ceria |
| url | https://hdl.handle.net/10356/160112 |
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