A H₂O₂-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging
Developing high-quality NIR-II fluorophores (emission in 1000-1700 nm) for in vivo imaging is of great significance. Benzothiadiazole-core fluorophores are an important class of NIR-II dyes, yet ongoing limitations such as aggregation-caused quenching in aqueous milieu and non-activatable response a...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/160405 |
| _version_ | 1811680194393538560 |
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| author | Chen, Junjie Chen, Longqi Wu, Yinglong Fang, Yichang Zeng, Fang Wu, Shuizhu Zhao, Yanli |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Chen, Junjie Chen, Longqi Wu, Yinglong Fang, Yichang Zeng, Fang Wu, Shuizhu Zhao, Yanli |
| author_sort | Chen, Junjie |
| collection | NTU |
| description | Developing high-quality NIR-II fluorophores (emission in 1000-1700 nm) for in vivo imaging is of great significance. Benzothiadiazole-core fluorophores are an important class of NIR-II dyes, yet ongoing limitations such as aggregation-caused quenching in aqueous milieu and non-activatable response are still major obstacles for their biological applications. Here, we devise an activatable nanoprobe to address these limitations. A molecular probe named BTPE-NO2 is synthesized by linking a benzothiadiazole core with two tetraphenylene groups serving as hydrophobic molecular rotors, followed by incorporating two nitrophenyloxoacetamide units at both ends of the core as recognition moieties and fluorescence quenchers. An FDA-approved amphiphilic polymer Pluronic F127 is then employed to encapsulate the molecular BTPE-NO2 to render the nanoprobe BTPE-NO2@F127. The pathological levels of H2O2 in the disease sites cleave the nitrophenyloxoacetamide groups and activate the probe, thereby generating strong fluorescent emission (950~1200 nm) and ultrasound signal for multi-mode imaging of inflammatory diseases. The nanoprobe can therefore function as a robust tool for detecting and imaging the disease sites with NIR-II fluorescent and multispectral optoacoustic tomography (MSOT) imaging. Moreover, the three-dimensional MSOT images can be obtained for visualizing and locating the disease foci. |
| first_indexed | 2024-10-01T03:21:10Z |
| format | Journal Article |
| id | ntu-10356/160405 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:21:10Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1604052023-02-28T19:59:54Z A H₂O₂-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging Chen, Junjie Chen, Longqi Wu, Yinglong Fang, Yichang Zeng, Fang Wu, Shuizhu Zhao, Yanli School of Physical and Mathematical Sciences Science::Chemistry Hydrogen Peroxide Optical Imaging Developing high-quality NIR-II fluorophores (emission in 1000-1700 nm) for in vivo imaging is of great significance. Benzothiadiazole-core fluorophores are an important class of NIR-II dyes, yet ongoing limitations such as aggregation-caused quenching in aqueous milieu and non-activatable response are still major obstacles for their biological applications. Here, we devise an activatable nanoprobe to address these limitations. A molecular probe named BTPE-NO2 is synthesized by linking a benzothiadiazole core with two tetraphenylene groups serving as hydrophobic molecular rotors, followed by incorporating two nitrophenyloxoacetamide units at both ends of the core as recognition moieties and fluorescence quenchers. An FDA-approved amphiphilic polymer Pluronic F127 is then employed to encapsulate the molecular BTPE-NO2 to render the nanoprobe BTPE-NO2@F127. The pathological levels of H2O2 in the disease sites cleave the nitrophenyloxoacetamide groups and activate the probe, thereby generating strong fluorescent emission (950~1200 nm) and ultrasound signal for multi-mode imaging of inflammatory diseases. The nanoprobe can therefore function as a robust tool for detecting and imaging the disease sites with NIR-II fluorescent and multispectral optoacoustic tomography (MSOT) imaging. Moreover, the three-dimensional MSOT images can be obtained for visualizing and locating the disease foci. Agency for Science, Technology and Research (A*STAR) National Research Foundation (NRF) Published version This work was supported by the National Natural Science Foundation of China (No. 21788102 to S.W. and No. 21875069 to F.Z.), the Natural Science Foundation of Guangdong Province (No. 2016A030312002 to S.W.), and the Fund of Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates (No. 2019B030301003 to S.W.). The work was also supported by the Singapore Agency for Science, Technology and Research (A*STAR) AME IRG grant (No. A20E5c0081 to Y.Z.) and the Singapore National Research Foundation Investigatorship (No. NRF-NRFI2018- 03 to Y.Z.). 2022-07-21T04:58:05Z 2022-07-21T04:58:05Z 2021 Journal Article Chen, J., Chen, L., Wu, Y., Fang, Y., Zeng, F., Wu, S. & Zhao, Y. (2021). A H₂O₂-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging. Nature Communications, 12(1), 6870-. https://dx.doi.org/10.1038/s41467-021-27233-4 2041-1723 https://hdl.handle.net/10356/160405 10.1038/s41467-021-27233-4 34824274 2-s2.0-85119849204 1 12 6870 en A20E5c0081 NRF-NRFI2018- 03 Nature Communications © 2021 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. application/pdf |
| spellingShingle | Science::Chemistry Hydrogen Peroxide Optical Imaging Chen, Junjie Chen, Longqi Wu, Yinglong Fang, Yichang Zeng, Fang Wu, Shuizhu Zhao, Yanli A H₂O₂-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging |
| title | A H₂O₂-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging |
| title_full | A H₂O₂-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging |
| title_fullStr | A H₂O₂-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging |
| title_full_unstemmed | A H₂O₂-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging |
| title_short | A H₂O₂-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging |
| title_sort | h₂o₂ activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia reperfusion injury via multispectral optoacoustic tomography and nir ii fluorescent imaging |
| topic | Science::Chemistry Hydrogen Peroxide Optical Imaging |
| url | https://hdl.handle.net/10356/160405 |
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