Bioelectroanalytical detection of lactic acid bacteria
Lactic acid bacteria (LAB) are an industrial important group of organisms that are notable for their inability to respire without growth supplements. Recently described bioelectroanalytical detectors that can specifically detect and enumerate microorganisms depend on a phenomenon known as extracellu...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/160632 |
| _version_ | 1811684951088693248 |
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| author | Han, Evelina Jing Ying Olias, Lola Gonzalez Wuertz, Stefan Hinks, Jamie |
| author2 | Singapore Eye Research Institute |
| author_facet | Singapore Eye Research Institute Han, Evelina Jing Ying Olias, Lola Gonzalez Wuertz, Stefan Hinks, Jamie |
| author_sort | Han, Evelina Jing Ying |
| collection | NTU |
| description | Lactic acid bacteria (LAB) are an industrial important group of organisms that are notable for their inability to respire without growth supplements. Recently described bioelectroanalytical detectors that can specifically detect and enumerate microorganisms depend on a phenomenon known as extracellular electron transport (EET) for effective detection. EET is often described as a type of microbial respiration, which logically excludes LAB from such a detection platform. However, members of the LAB have recently been described as electroactive with the ability to carry out EET, providing a timely impetus to revisit the utility of bioelectroanalytical detectors in LAB detection. Here, we show that an LAB, Enterococcus faecalis, is easily detected bioelectroanalytically using the defined substrate resorufin-β-D-galactopyranoside. Detection is rapid, ranging from 34 to 235 min for inoculum sizes between 107 and 104 CFU mL−1, respectively. We show that, although the signal achieved by Enterococcus faecalis is comparable to systems that rely on the respiratory EET strategies of target bacteria, E. faecalis is not dependent on the electrode for energy, and it is only necessary to capture small amounts of an organism’s metabolic energy to, in this case 1.6%, to achieve good detection. The results pave the way for new means of detecting an industrially important group of organisms, particularly in the food industry. |
| first_indexed | 2024-10-01T04:36:47Z |
| format | Journal Article |
| id | ntu-10356/160632 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:36:47Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1606322022-07-30T20:12:22Z Bioelectroanalytical detection of lactic acid bacteria Han, Evelina Jing Ying Olias, Lola Gonzalez Wuertz, Stefan Hinks, Jamie Singapore Eye Research Institute Singapore Centre for Environmental Life Sciences and Engineering Science::Biological sciences Lactic Acid Bacteria Extracellular Electron Transfer Lactic acid bacteria (LAB) are an industrial important group of organisms that are notable for their inability to respire without growth supplements. Recently described bioelectroanalytical detectors that can specifically detect and enumerate microorganisms depend on a phenomenon known as extracellular electron transport (EET) for effective detection. EET is often described as a type of microbial respiration, which logically excludes LAB from such a detection platform. However, members of the LAB have recently been described as electroactive with the ability to carry out EET, providing a timely impetus to revisit the utility of bioelectroanalytical detectors in LAB detection. Here, we show that an LAB, Enterococcus faecalis, is easily detected bioelectroanalytically using the defined substrate resorufin-β-D-galactopyranoside. Detection is rapid, ranging from 34 to 235 min for inoculum sizes between 107 and 104 CFU mL−1, respectively. We show that, although the signal achieved by Enterococcus faecalis is comparable to systems that rely on the respiratory EET strategies of target bacteria, E. faecalis is not dependent on the electrode for energy, and it is only necessary to capture small amounts of an organism’s metabolic energy to, in this case 1.6%, to achieve good detection. The results pave the way for new means of detecting an industrially important group of organisms, particularly in the food industry. Ministry of Education (MOE) Nanyang Technological University National Research Foundation (NRF) Published version This work was supported by seed funding from NTUs Sustainable Earth Office (SEO), NRF gap fund ignition grant (NGF-2016-01-013) administered by NTUitive Ltd., and SCELSE core funds. SCELSE is funded by the National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS) and hosted by NTU in partnership with NUS. 2022-07-29T01:01:45Z 2022-07-29T01:01:45Z 2022 Journal Article Han, E. J. Y., Olias, L. G., Wuertz, S. & Hinks, J. (2022). Bioelectroanalytical detection of lactic acid bacteria. Applied Sciences, 12(3), 1257-. https://dx.doi.org/10.3390/app12031257 2076-3417 https://hdl.handle.net/10356/160632 10.3390/app12031257 2-s2.0-85123439499 3 12 1257 en NGF-2016-01-013 Applied Sciences © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
| spellingShingle | Science::Biological sciences Lactic Acid Bacteria Extracellular Electron Transfer Han, Evelina Jing Ying Olias, Lola Gonzalez Wuertz, Stefan Hinks, Jamie Bioelectroanalytical detection of lactic acid bacteria |
| title | Bioelectroanalytical detection of lactic acid bacteria |
| title_full | Bioelectroanalytical detection of lactic acid bacteria |
| title_fullStr | Bioelectroanalytical detection of lactic acid bacteria |
| title_full_unstemmed | Bioelectroanalytical detection of lactic acid bacteria |
| title_short | Bioelectroanalytical detection of lactic acid bacteria |
| title_sort | bioelectroanalytical detection of lactic acid bacteria |
| topic | Science::Biological sciences Lactic Acid Bacteria Extracellular Electron Transfer |
| url | https://hdl.handle.net/10356/160632 |
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