Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves
Precise, automatic and reliable position control of micro-objects such as single particles, biological cells or bio-organisms is critical for applications in biotechnology and tissue engineering. However, conventional acoustofluidic techniques generally lack reliability and automation capability thu...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/160976 |
| _version_ | 1811677891958669312 |
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| author | Nguyen, Tan Dai Fu, Yong Qing Tran, Van-Thai Gautam, Archana Pudasaini, Sanam Du, Hejun |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Nguyen, Tan Dai Fu, Yong Qing Tran, Van-Thai Gautam, Archana Pudasaini, Sanam Du, Hejun |
| author_sort | Nguyen, Tan Dai |
| collection | NTU |
| description | Precise, automatic and reliable position control of micro-objects such as single particles, biological cells or bio-organisms is critical for applications in biotechnology and tissue engineering. However, conventional acoustofluidic techniques generally lack reliability and automation capability thus are often incapable of building an efficient and automated system where the biological cells need to be precisely manipulated in three dimensions (3D). To overcome these limitations, we developed an acoustofluidic closed-loop control system which is combined with computer vision techniques and standing surface acoustic waves (SSAWs) to implement selective, automatic and precise position control of an object, such as a single cell or microparticle in a microfluidic chamber. Position of the object is in situ extracted from living images that are captured from a video camera. By utilizing the closed-loop control strategy, the object is precisely moved to the desired location in 3D patterns or along designed trajectories by manipulating the phase angle and power signal of the SSAWs. Controlling of breast cancer cells has been conducted to verify the principle and biocompatibility of the control system. This system could be employed to build an automatic system for cell analysis, cell isolation, self-assembling of materials into complex microstructures, or lab-on-chip and organ-on-chip applications. |
| first_indexed | 2024-10-01T02:44:35Z |
| format | Journal Article |
| id | ntu-10356/160976 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:44:35Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1609762022-08-10T02:51:07Z Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves Nguyen, Tan Dai Fu, Yong Qing Tran, Van-Thai Gautam, Archana Pudasaini, Sanam Du, Hejun School of Mechanical and Aerospace Engineering Engineering::Mechanical engineering Microfluidics Acoustofluidics Precise, automatic and reliable position control of micro-objects such as single particles, biological cells or bio-organisms is critical for applications in biotechnology and tissue engineering. However, conventional acoustofluidic techniques generally lack reliability and automation capability thus are often incapable of building an efficient and automated system where the biological cells need to be precisely manipulated in three dimensions (3D). To overcome these limitations, we developed an acoustofluidic closed-loop control system which is combined with computer vision techniques and standing surface acoustic waves (SSAWs) to implement selective, automatic and precise position control of an object, such as a single cell or microparticle in a microfluidic chamber. Position of the object is in situ extracted from living images that are captured from a video camera. By utilizing the closed-loop control strategy, the object is precisely moved to the desired location in 3D patterns or along designed trajectories by manipulating the phase angle and power signal of the SSAWs. Controlling of breast cancer cells has been conducted to verify the principle and biocompatibility of the control system. This system could be employed to build an automatic system for cell analysis, cell isolation, self-assembling of materials into complex microstructures, or lab-on-chip and organ-on-chip applications. Ministry of Education (MOE) Nanyang Technological University The authors gratefully acknowledge the support of (i) Nanyang Technological University and the Ministry of Education of Singapore through a PhD Scholarship; (ii) the UK Engineering and Physical Sciences Research Council (EPSRC) grants EP/P018998/1; (iii) Special Interesting Group of Acoustofluidics funded by UK Fluids Network (EP/N032861/1). 2022-08-10T02:51:07Z 2022-08-10T02:51:07Z 2020 Journal Article Nguyen, T. D., Fu, Y. Q., Tran, V., Gautam, A., Pudasaini, S. & Du, H. (2020). Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves. Sensors and Actuators, B: Chemical, 318, 128143-. https://dx.doi.org/10.1016/j.snb.2020.128143 0925-4005 https://hdl.handle.net/10356/160976 10.1016/j.snb.2020.128143 2-s2.0-85085362848 318 128143 en Sensors and Actuators, B: Chemical © 2020 Elsevier B.V. All rights reserved. |
| spellingShingle | Engineering::Mechanical engineering Microfluidics Acoustofluidics Nguyen, Tan Dai Fu, Yong Qing Tran, Van-Thai Gautam, Archana Pudasaini, Sanam Du, Hejun Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves |
| title | Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves |
| title_full | Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves |
| title_fullStr | Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves |
| title_full_unstemmed | Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves |
| title_short | Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves |
| title_sort | acoustofluidic closed loop control of microparticles and cells using standing surface acoustic waves |
| topic | Engineering::Mechanical engineering Microfluidics Acoustofluidics |
| url | https://hdl.handle.net/10356/160976 |
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