Controlling droplet impact velocity and droplet volume: key factors to achieving high cell viability in sub-nanoliter droplet-based bioprinting
Three-dimensional (3D) bioprinting systems serve as advanced manufacturing platform for the precise deposition of cells and biomaterials at pre-defined positions. Among the various bioprinting techniques, the drop-on-demand jetting approach facilitates deposition of pico/nanoliter droplets of cells...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/161002 |
| _version_ | 1826120947563757568 |
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| author | Ng, Wei Long Huang, Xi Shkolnikov, Viktor Goh, Guo Liang Suntornnond, Ratima Yeong, Wai Yee |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Ng, Wei Long Huang, Xi Shkolnikov, Viktor Goh, Guo Liang Suntornnond, Ratima Yeong, Wai Yee |
| author_sort | Ng, Wei Long |
| collection | NTU |
| description | Three-dimensional (3D) bioprinting systems serve as advanced manufacturing platform for the precise deposition of cells and biomaterials at pre-defined positions. Among the various bioprinting techniques, the drop-on-demand jetting approach facilitates deposition of pico/nanoliter droplets of cells and materials for study of cell-cell and cell-matrix interactions. Despite advances in the bioprinting systems, there is a poor understanding of how the viability of primary human cells within sub-nanoliter droplets is affected during the printing process. In this work, a thermal inkjet system is utilized to dispense sub-nanoliter cell-laden droplets, and two key factors - droplet impact velocity and droplet volume - are identified to have significant effect on the viability and proliferation of printed cells. An increase in the cell concentration results in slower impact velocity, which leads to higher viability of the printed cells and improves the printing outcome by mitigating droplet splashing. Furthermore, a minimum droplet volume of 20 nL per spot helps to mitigate evaporation-induced cell damage and maintain high viability of the printed cells within a printing duration of 2 min. Hence, controlling the droplet impact velocity and droplet volume in sub-nanoliter bioprinting is critical for viability and proliferation of printed human primary cells. |
| first_indexed | 2024-10-01T05:24:46Z |
| format | Journal Article |
| id | ntu-10356/161002 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:24:46Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1610022023-11-03T02:12:22Z Controlling droplet impact velocity and droplet volume: key factors to achieving high cell viability in sub-nanoliter droplet-based bioprinting Ng, Wei Long Huang, Xi Shkolnikov, Viktor Goh, Guo Liang Suntornnond, Ratima Yeong, Wai Yee School of Mechanical and Aerospace Engineering Singapore Centre for 3D Printing HP-NTU Digital Manufacturing Corporate Lab Engineering::Mechanical engineering 3D Bioprinting 3D printing Three-dimensional (3D) bioprinting systems serve as advanced manufacturing platform for the precise deposition of cells and biomaterials at pre-defined positions. Among the various bioprinting techniques, the drop-on-demand jetting approach facilitates deposition of pico/nanoliter droplets of cells and materials for study of cell-cell and cell-matrix interactions. Despite advances in the bioprinting systems, there is a poor understanding of how the viability of primary human cells within sub-nanoliter droplets is affected during the printing process. In this work, a thermal inkjet system is utilized to dispense sub-nanoliter cell-laden droplets, and two key factors - droplet impact velocity and droplet volume - are identified to have significant effect on the viability and proliferation of printed cells. An increase in the cell concentration results in slower impact velocity, which leads to higher viability of the printed cells and improves the printing outcome by mitigating droplet splashing. Furthermore, a minimum droplet volume of 20 nL per spot helps to mitigate evaporation-induced cell damage and maintain high viability of the printed cells within a printing duration of 2 min. Hence, controlling the droplet impact velocity and droplet volume in sub-nanoliter bioprinting is critical for viability and proliferation of printed human primary cells. Published version This study is supported under the RIE2020 Industry Alignment Fund – Industry Collaboration Projects (IAFICP) Funding Initiative, as well as cash and in-kind contribution from the industry partner, HP Inc., through the HP-NTU Digital Manufacturing Corporate Lab. 2022-08-11T06:21:02Z 2022-08-11T06:21:02Z 2022 Journal Article Ng, W. L., Huang, X., Shkolnikov, V., Goh, G. L., Suntornnond, R. & Yeong, W. Y. (2022). Controlling droplet impact velocity and droplet volume: key factors to achieving high cell viability in sub-nanoliter droplet-based bioprinting. International Journal of Bioprinting, 8(1), 424-. https://dx.doi.org/10.18063/ijb.v8i1.424 2424-8002 https://hdl.handle.net/10356/161002 10.18063/ijb.v8i1.424 35187273 1 8 424 en International Journal of Bioprinting © 2021 Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution and reproduction in any medium, provided the original work is properly cited. application/pdf |
| spellingShingle | Engineering::Mechanical engineering 3D Bioprinting 3D printing Ng, Wei Long Huang, Xi Shkolnikov, Viktor Goh, Guo Liang Suntornnond, Ratima Yeong, Wai Yee Controlling droplet impact velocity and droplet volume: key factors to achieving high cell viability in sub-nanoliter droplet-based bioprinting |
| title | Controlling droplet impact velocity and droplet volume: key factors to achieving high cell viability in sub-nanoliter droplet-based bioprinting |
| title_full | Controlling droplet impact velocity and droplet volume: key factors to achieving high cell viability in sub-nanoliter droplet-based bioprinting |
| title_fullStr | Controlling droplet impact velocity and droplet volume: key factors to achieving high cell viability in sub-nanoliter droplet-based bioprinting |
| title_full_unstemmed | Controlling droplet impact velocity and droplet volume: key factors to achieving high cell viability in sub-nanoliter droplet-based bioprinting |
| title_short | Controlling droplet impact velocity and droplet volume: key factors to achieving high cell viability in sub-nanoliter droplet-based bioprinting |
| title_sort | controlling droplet impact velocity and droplet volume key factors to achieving high cell viability in sub nanoliter droplet based bioprinting |
| topic | Engineering::Mechanical engineering 3D Bioprinting 3D printing |
| url | https://hdl.handle.net/10356/161002 |
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