The CbbQO-type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form IA rubiscos
The CO2-fixing enzyme rubisco is responsible for almost all carbon fixation. This process frequently requires rubisco activase (Rca) machinery, which couples ATP hydrolysis to the removal of inhibitory sugar phosphates, including the rubisco substrate ribulose 1,5-bisphosphate (RuBP). Rubisco is som...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | English |
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2022
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| Online Access: | https://hdl.handle.net/10356/161127 |
| _version_ | 1811684970066870272 |
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| author | Tsai, Candace Yi-Chin Liew, Lynette Guo, Zhijun Liu, Di Mueller-Cajar, Oliver |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Tsai, Candace Yi-Chin Liew, Lynette Guo, Zhijun Liu, Di Mueller-Cajar, Oliver |
| author_sort | Tsai, Candace Yi-Chin |
| collection | NTU |
| description | The CO2-fixing enzyme rubisco is responsible for almost all carbon fixation. This process frequently requires rubisco activase (Rca) machinery, which couples ATP hydrolysis to the removal of inhibitory sugar phosphates, including the rubisco substrate ribulose 1,5-bisphosphate (RuBP). Rubisco is sometimes compartmentalized in carboxysomes, bacterial microcompartments that enable a carbon dioxide concentrating mechanism (CCM). Characterized carboxysomal rubiscos, however, are not prone to inhibition, and often no activase machinery is associated with these enzymes. Here, we characterize two carboxysomal rubiscos of the form IAC clade that are associated with CbbQO-type Rcas. These enzymes release RuBP at a much lower rate than the canonical carboxysomal rubisco from Synechococcus PCC6301. We found that CbbQO-type Rcas encoded in carboxysome gene clusters can remove RuBP and the tight-binding transition state analog carboxy-arabinitol 1,5-bisphosphate from cognate rubiscos. The Acidithiobacillus ferrooxidans genome encodes two form IA rubiscos associated with two sets of cbbQ and cbbO genes. We show that the two CbbQO activase systems display specificity for the rubisco enzyme encoded in the same gene cluster, and this property can be switched by substituting the C-terminal three residues of the large subunit. Our findings indicate that the kinetic and inhibitory properties of proteobacterial form IA rubiscos are diverse and predict that Rcas may be necessary for some α-carboxysomal CCMs. These findings will have implications for efforts aiming to introduce biophysical CCMs into plants and other hosts for improvement of carbon fixation of crops. |
| first_indexed | 2024-10-01T04:37:05Z |
| format | Journal Article |
| id | ntu-10356/161127 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:37:05Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1611272023-02-28T17:13:33Z The CbbQO-type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form IA rubiscos Tsai, Candace Yi-Chin Liew, Lynette Guo, Zhijun Liu, Di Mueller-Cajar, Oliver School of Biological Sciences Science::Biological sciences Rubisco Rubisco Activase The CO2-fixing enzyme rubisco is responsible for almost all carbon fixation. This process frequently requires rubisco activase (Rca) machinery, which couples ATP hydrolysis to the removal of inhibitory sugar phosphates, including the rubisco substrate ribulose 1,5-bisphosphate (RuBP). Rubisco is sometimes compartmentalized in carboxysomes, bacterial microcompartments that enable a carbon dioxide concentrating mechanism (CCM). Characterized carboxysomal rubiscos, however, are not prone to inhibition, and often no activase machinery is associated with these enzymes. Here, we characterize two carboxysomal rubiscos of the form IAC clade that are associated with CbbQO-type Rcas. These enzymes release RuBP at a much lower rate than the canonical carboxysomal rubisco from Synechococcus PCC6301. We found that CbbQO-type Rcas encoded in carboxysome gene clusters can remove RuBP and the tight-binding transition state analog carboxy-arabinitol 1,5-bisphosphate from cognate rubiscos. The Acidithiobacillus ferrooxidans genome encodes two form IA rubiscos associated with two sets of cbbQ and cbbO genes. We show that the two CbbQO activase systems display specificity for the rubisco enzyme encoded in the same gene cluster, and this property can be switched by substituting the C-terminal three residues of the large subunit. Our findings indicate that the kinetic and inhibitory properties of proteobacterial form IA rubiscos are diverse and predict that Rcas may be necessary for some α-carboxysomal CCMs. These findings will have implications for efforts aiming to introduce biophysical CCMs into plants and other hosts for improvement of carbon fixation of crops. Ministry of Education (MOE) National Research Foundation (NRF) Published version This work was funded by Ministry of Education (MOE) of Singapore tier 2 grants (MOE2016- T2-2-088 and MOE-T2EP30120-0005) and the National Research Foundation (NRF) of Singapore grant (NRF2017-NRF-ISF002-2667) (to O. M.-C.). 2022-08-16T07:00:28Z 2022-08-16T07:00:28Z 2022 Journal Article Tsai, C. Y., Liew, L., Guo, Z., Liu, D. & Mueller-Cajar, O. (2022). The CbbQO-type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form IA rubiscos. Journal of Biological Chemistry, 298(1), 101476-. https://dx.doi.org/10.1016/j.jbc.2021.101476 0021-9258 https://hdl.handle.net/10356/161127 10.1016/j.jbc.2021.101476 34890642 2-s2.0-85122060079 1 298 101476 en MOE2016- T2-2-088 MOE-T2EP30120-0005 NRF2017-NRF-ISF002-2667 Journal of Biological Chemistry © 2021 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). application/pdf |
| spellingShingle | Science::Biological sciences Rubisco Rubisco Activase Tsai, Candace Yi-Chin Liew, Lynette Guo, Zhijun Liu, Di Mueller-Cajar, Oliver The CbbQO-type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form IA rubiscos |
| title | The CbbQO-type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form IA rubiscos |
| title_full | The CbbQO-type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form IA rubiscos |
| title_fullStr | The CbbQO-type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form IA rubiscos |
| title_full_unstemmed | The CbbQO-type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form IA rubiscos |
| title_short | The CbbQO-type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form IA rubiscos |
| title_sort | cbbqo type rubisco activases encoded in carboxysome gene clusters can activate carboxysomal form ia rubiscos |
| topic | Science::Biological sciences Rubisco Rubisco Activase |
| url | https://hdl.handle.net/10356/161127 |
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