Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification
Aerobic denitrification is attracting increasing attention since its advantage of complete nitrogen removal in a single aerobic reactor with simplified configurations. This study investigated the nitrate kinetic affinity (half-saturation index, Km) by an isolated aerobic denitrifier named P. baleari...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/161204 |
| _version_ | 1826117670446039040 |
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| author | Ruan, Yunjie Ma, Bin Cai, Chen Cai, Lei Gu, Jun Lu, Hui-Feng Xu, Xiang-Yang Zhang, Meng |
| author2 | Nanyang Environment and Water Research Institute |
| author_facet | Nanyang Environment and Water Research Institute Ruan, Yunjie Ma, Bin Cai, Chen Cai, Lei Gu, Jun Lu, Hui-Feng Xu, Xiang-Yang Zhang, Meng |
| author_sort | Ruan, Yunjie |
| collection | NTU |
| description | Aerobic denitrification is attracting increasing attention since its advantage of complete nitrogen removal in a single aerobic reactor with simplified configurations. This study investigated the nitrate kinetic affinity (half-saturation index, Km) by an isolated aerobic denitrifier named P. balearica strain RAD-17. It turned out that strain RAD-17 had a high Km of 162.5 mg-N/L and maximum nitrate reduction rate of 21.7 mg-N/(L•h), enabling it to treat high-strength nitrogen wastewater with high efficiency. Further analysis illustrated that Km was the critical value for the change of growth yield rate along initial nitrate concentrations. Nitrogen balance results elucidated an opposite nitrogen flux to cell synthesis and nitrogen loss during aerobic denitrification. Moreover, the expression of functional genes provided proofs for these phenotypic results at transcriptional level. Consequently, Km could be an indicator for nitrate flux division directing to respiration and assimilation in aerobic denitrifiers, shedding light on its regulation for wastewater treatment. |
| first_indexed | 2024-10-01T04:31:06Z |
| format | Journal Article |
| id | ntu-10356/161204 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:31:06Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1612042022-08-19T05:12:21Z Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification Ruan, Yunjie Ma, Bin Cai, Chen Cai, Lei Gu, Jun Lu, Hui-Feng Xu, Xiang-Yang Zhang, Meng Nanyang Environment and Water Research Institute Engineering::Environmental engineering Aerobic Denitrification Kinetic Affinity Aerobic denitrification is attracting increasing attention since its advantage of complete nitrogen removal in a single aerobic reactor with simplified configurations. This study investigated the nitrate kinetic affinity (half-saturation index, Km) by an isolated aerobic denitrifier named P. balearica strain RAD-17. It turned out that strain RAD-17 had a high Km of 162.5 mg-N/L and maximum nitrate reduction rate of 21.7 mg-N/(L•h), enabling it to treat high-strength nitrogen wastewater with high efficiency. Further analysis illustrated that Km was the critical value for the change of growth yield rate along initial nitrate concentrations. Nitrogen balance results elucidated an opposite nitrogen flux to cell synthesis and nitrogen loss during aerobic denitrification. Moreover, the expression of functional genes provided proofs for these phenotypic results at transcriptional level. Consequently, Km could be an indicator for nitrate flux division directing to respiration and assimilation in aerobic denitrifiers, shedding light on its regulation for wastewater treatment. This study was financially supported by National Natural Science Fundation of China (31972842, 51608474) and Primary Research and Development Plan of Zhejiang Province (2018C02037). 2022-08-19T05:12:21Z 2022-08-19T05:12:21Z 2020 Journal Article Ruan, Y., Ma, B., Cai, C., Cai, L., Gu, J., Lu, H., Xu, X. & Zhang, M. (2020). Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification. Bioresource Technology, 309, 123345-. https://dx.doi.org/10.1016/j.biortech.2020.123345 0960-8524 https://hdl.handle.net/10356/161204 10.1016/j.biortech.2020.123345 32305844 2-s2.0-85083295180 309 123345 en Bioresource Technology © 2020 Elsevier Ltd. All rights reserved. |
| spellingShingle | Engineering::Environmental engineering Aerobic Denitrification Kinetic Affinity Ruan, Yunjie Ma, Bin Cai, Chen Cai, Lei Gu, Jun Lu, Hui-Feng Xu, Xiang-Yang Zhang, Meng Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification |
| title | Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification |
| title_full | Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification |
| title_fullStr | Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification |
| title_full_unstemmed | Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification |
| title_short | Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification |
| title_sort | kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification |
| topic | Engineering::Environmental engineering Aerobic Denitrification Kinetic Affinity |
| url | https://hdl.handle.net/10356/161204 |
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