Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification

Aerobic denitrification is attracting increasing attention since its advantage of complete nitrogen removal in a single aerobic reactor with simplified configurations. This study investigated the nitrate kinetic affinity (half-saturation index, Km) by an isolated aerobic denitrifier named P. baleari...

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Main Authors: Ruan, Yunjie, Ma, Bin, Cai, Chen, Cai, Lei, Gu, Jun, Lu, Hui-Feng, Xu, Xiang-Yang, Zhang, Meng
Other Authors: Nanyang Environment and Water Research Institute
Format: Journal Article
Language:English
Published: 2022
Subjects:
Online Access:https://hdl.handle.net/10356/161204
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author Ruan, Yunjie
Ma, Bin
Cai, Chen
Cai, Lei
Gu, Jun
Lu, Hui-Feng
Xu, Xiang-Yang
Zhang, Meng
author2 Nanyang Environment and Water Research Institute
author_facet Nanyang Environment and Water Research Institute
Ruan, Yunjie
Ma, Bin
Cai, Chen
Cai, Lei
Gu, Jun
Lu, Hui-Feng
Xu, Xiang-Yang
Zhang, Meng
author_sort Ruan, Yunjie
collection NTU
description Aerobic denitrification is attracting increasing attention since its advantage of complete nitrogen removal in a single aerobic reactor with simplified configurations. This study investigated the nitrate kinetic affinity (half-saturation index, Km) by an isolated aerobic denitrifier named P. balearica strain RAD-17. It turned out that strain RAD-17 had a high Km of 162.5 mg-N/L and maximum nitrate reduction rate of 21.7 mg-N/(L•h), enabling it to treat high-strength nitrogen wastewater with high efficiency. Further analysis illustrated that Km was the critical value for the change of growth yield rate along initial nitrate concentrations. Nitrogen balance results elucidated an opposite nitrogen flux to cell synthesis and nitrogen loss during aerobic denitrification. Moreover, the expression of functional genes provided proofs for these phenotypic results at transcriptional level. Consequently, Km could be an indicator for nitrate flux division directing to respiration and assimilation in aerobic denitrifiers, shedding light on its regulation for wastewater treatment.
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spelling ntu-10356/1612042022-08-19T05:12:21Z Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification Ruan, Yunjie Ma, Bin Cai, Chen Cai, Lei Gu, Jun Lu, Hui-Feng Xu, Xiang-Yang Zhang, Meng Nanyang Environment and Water Research Institute Engineering::Environmental engineering Aerobic Denitrification Kinetic Affinity Aerobic denitrification is attracting increasing attention since its advantage of complete nitrogen removal in a single aerobic reactor with simplified configurations. This study investigated the nitrate kinetic affinity (half-saturation index, Km) by an isolated aerobic denitrifier named P. balearica strain RAD-17. It turned out that strain RAD-17 had a high Km of 162.5 mg-N/L and maximum nitrate reduction rate of 21.7 mg-N/(L•h), enabling it to treat high-strength nitrogen wastewater with high efficiency. Further analysis illustrated that Km was the critical value for the change of growth yield rate along initial nitrate concentrations. Nitrogen balance results elucidated an opposite nitrogen flux to cell synthesis and nitrogen loss during aerobic denitrification. Moreover, the expression of functional genes provided proofs for these phenotypic results at transcriptional level. Consequently, Km could be an indicator for nitrate flux division directing to respiration and assimilation in aerobic denitrifiers, shedding light on its regulation for wastewater treatment. This study was financially supported by National Natural Science Fundation of China (31972842, 51608474) and Primary Research and Development Plan of Zhejiang Province (2018C02037). 2022-08-19T05:12:21Z 2022-08-19T05:12:21Z 2020 Journal Article Ruan, Y., Ma, B., Cai, C., Cai, L., Gu, J., Lu, H., Xu, X. & Zhang, M. (2020). Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification. Bioresource Technology, 309, 123345-. https://dx.doi.org/10.1016/j.biortech.2020.123345 0960-8524 https://hdl.handle.net/10356/161204 10.1016/j.biortech.2020.123345 32305844 2-s2.0-85083295180 309 123345 en Bioresource Technology © 2020 Elsevier Ltd. All rights reserved.
spellingShingle Engineering::Environmental engineering
Aerobic Denitrification
Kinetic Affinity
Ruan, Yunjie
Ma, Bin
Cai, Chen
Cai, Lei
Gu, Jun
Lu, Hui-Feng
Xu, Xiang-Yang
Zhang, Meng
Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification
title Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification
title_full Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification
title_fullStr Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification
title_full_unstemmed Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification
title_short Kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification
title_sort kinetic affinity index informs the divisions of nitrate flux in aerobic denitrification
topic Engineering::Environmental engineering
Aerobic Denitrification
Kinetic Affinity
url https://hdl.handle.net/10356/161204
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