Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis
Vegetable oils are not only major components of human diet but also vital for industrial applications. WRINKLED1 (WRI1) is a pivotal transcription factor governing plant oil biosynthesis, but the underlying DNA-binding mechanism remains incompletely understood. Here, we resolved the structure of Ara...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/161321 |
| _version_ | 1826116225744240640 |
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| author | Qiao, Zhu Kong, Que Tee, Wan Ting Lim, Audrey R. Q. Teo, Miao Xuan Olieric, Vincent Low, Pui Man Yang, Yuzhou Qian, Guoliang Ma, Wei Gao, Yong-Gui |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Qiao, Zhu Kong, Que Tee, Wan Ting Lim, Audrey R. Q. Teo, Miao Xuan Olieric, Vincent Low, Pui Man Yang, Yuzhou Qian, Guoliang Ma, Wei Gao, Yong-Gui |
| author_sort | Qiao, Zhu |
| collection | NTU |
| description | Vegetable oils are not only major components of human diet but also vital for industrial applications. WRINKLED1 (WRI1) is a pivotal transcription factor governing plant oil biosynthesis, but the underlying DNA-binding mechanism remains incompletely understood. Here, we resolved the structure of Arabidopsis WRI1 (AtWRI1) with its cognate double-stranded DNA (dsDNA), revealing two antiparallel β sheets in the tandem AP2 domains that intercalate into the adjacent major grooves of dsDNA to determine the sequence recognition specificity. We showed that AtWRI1 represented a previously unidentified structural fold and DNA-binding mode. Mutations of the key residues interacting with DNA element affected its binding affinity and oil biosynthesis when these variants were transiently expressed in tobacco leaves. Seed oil content was enhanced in stable transgenic wri1-1 expressing an AtWRI1 variant (W74R). Together, our findings offer a structural basis explaining WRI1 recognition and binding of DNA and suggest an alternative strategy to increase oil yield in crops through WRI1 bioengineering. |
| first_indexed | 2024-10-01T04:07:51Z |
| format | Journal Article |
| id | ntu-10356/161321 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:07:51Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1613212023-02-28T17:13:51Z Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis Qiao, Zhu Kong, Que Tee, Wan Ting Lim, Audrey R. Q. Teo, Miao Xuan Olieric, Vincent Low, Pui Man Yang, Yuzhou Qian, Guoliang Ma, Wei Gao, Yong-Gui School of Biological Sciences NTU Institute of Structural Biology Science::Biological sciences Plant Oil WRINKLED1 Vegetable oils are not only major components of human diet but also vital for industrial applications. WRINKLED1 (WRI1) is a pivotal transcription factor governing plant oil biosynthesis, but the underlying DNA-binding mechanism remains incompletely understood. Here, we resolved the structure of Arabidopsis WRI1 (AtWRI1) with its cognate double-stranded DNA (dsDNA), revealing two antiparallel β sheets in the tandem AP2 domains that intercalate into the adjacent major grooves of dsDNA to determine the sequence recognition specificity. We showed that AtWRI1 represented a previously unidentified structural fold and DNA-binding mode. Mutations of the key residues interacting with DNA element affected its binding affinity and oil biosynthesis when these variants were transiently expressed in tobacco leaves. Seed oil content was enhanced in stable transgenic wri1-1 expressing an AtWRI1 variant (W74R). Together, our findings offer a structural basis explaining WRI1 recognition and binding of DNA and suggest an alternative strategy to increase oil yield in crops through WRI1 bioengineering. Ministry of Education (MOE) Published version This work was supported by Ministry of Education (MOE) of Singapore Tier 2 (MOE-2019-T2-099) to Y.-G.G., MOE of Singapore Tier 1 (RG29/20) to W.M., and MOE of Singapore Tier 2 (MOE-T2EP30220-0011) to W.M. 2022-08-25T07:00:29Z 2022-08-25T07:00:29Z 2022 Journal Article Qiao, Z., Kong, Q., Tee, W. T., Lim, A. R. Q., Teo, M. X., Olieric, V., Low, P. M., Yang, Y., Qian, G., Ma, W. & Gao, Y. (2022). Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis. Science Advances, 8(34), eabq1211-. https://dx.doi.org/10.1126/sciadv.abq1211 2375-2548 https://hdl.handle.net/10356/161321 10.1126/sciadv.abq1211 34 8 eabq1211 en MOE-2019-T2-099 RG29/20 MOE-T2EP30220-0011 Science Advances © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). application/pdf |
| spellingShingle | Science::Biological sciences Plant Oil WRINKLED1 Qiao, Zhu Kong, Que Tee, Wan Ting Lim, Audrey R. Q. Teo, Miao Xuan Olieric, Vincent Low, Pui Man Yang, Yuzhou Qian, Guoliang Ma, Wei Gao, Yong-Gui Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis |
| title | Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis |
| title_full | Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis |
| title_fullStr | Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis |
| title_full_unstemmed | Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis |
| title_short | Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis |
| title_sort | molecular basis of the key regulator wrinkled1 in plant oil biosynthesis |
| topic | Science::Biological sciences Plant Oil WRINKLED1 |
| url | https://hdl.handle.net/10356/161321 |
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