Coassembly of a new insect cuticular protein and chitosan via liquid–liquid phase separation
Insect cuticle is a fiber-reinforced composite material that consists of polysaccharide chitin fibers and a protein matrix. The molecular interactions between insect cuticle proteins and chitin that govern the assembly and evolution of cuticle are still not well understood. Herein, we report that Os...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/161612 |
| _version_ | 1826115219763494912 |
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| author | Gong, Qiuyu Chen, Lei Wang, Jining Yuan, Fenghou Ma, Zhiming Chen, Guoxin Huang, Yinjuan Miao, Yansong Liu, Tian Zhang, Xin-Xing Yang, Qing Yu, Jing |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Gong, Qiuyu Chen, Lei Wang, Jining Yuan, Fenghou Ma, Zhiming Chen, Guoxin Huang, Yinjuan Miao, Yansong Liu, Tian Zhang, Xin-Xing Yang, Qing Yu, Jing |
| author_sort | Gong, Qiuyu |
| collection | NTU |
| description | Insect cuticle is a fiber-reinforced composite material that consists of polysaccharide chitin fibers and a protein matrix. The molecular interactions between insect cuticle proteins and chitin that govern the assembly and evolution of cuticle are still not well understood. Herein, we report that Ostrinia furnacalis cuticular protein hypothetical-1 (OfCPH-1), a newly discovered and most abundant cuticular protein from Asian corn borer Ostrinia furnacalis, can form coacervates in the presence of chitosan. The OfCPH-1-chitosan coacervate micro-droplets are initially liquid-like, but become gel-like with increasing time or salt concentration. The liquid-to-gel transition is driven by hydrogen-bonding interactions, during which an induced β-sheet structure of OfCPH-1 is observed. Given the abundance of OfCPH-1 in the cuticle of Ostrinia furnacalis, this liquid-liquid phase separation process and its aging behavior could play critical roles in the formation of the cuticle. |
| first_indexed | 2024-10-01T03:51:39Z |
| format | Journal Article |
| id | ntu-10356/161612 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:51:39Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1616122022-09-17T23:31:26Z Coassembly of a new insect cuticular protein and chitosan via liquid–liquid phase separation Gong, Qiuyu Chen, Lei Wang, Jining Yuan, Fenghou Ma, Zhiming Chen, Guoxin Huang, Yinjuan Miao, Yansong Liu, Tian Zhang, Xin-Xing Yang, Qing Yu, Jing School of Materials Science and Engineering School of Biological Sciences Nanyang Environment and Water Research Institute Singapore Membrane Technology Centre Science::Biological sciences Chitosan Peptides and Proteins Insect cuticle is a fiber-reinforced composite material that consists of polysaccharide chitin fibers and a protein matrix. The molecular interactions between insect cuticle proteins and chitin that govern the assembly and evolution of cuticle are still not well understood. Herein, we report that Ostrinia furnacalis cuticular protein hypothetical-1 (OfCPH-1), a newly discovered and most abundant cuticular protein from Asian corn borer Ostrinia furnacalis, can form coacervates in the presence of chitosan. The OfCPH-1-chitosan coacervate micro-droplets are initially liquid-like, but become gel-like with increasing time or salt concentration. The liquid-to-gel transition is driven by hydrogen-bonding interactions, during which an induced β-sheet structure of OfCPH-1 is observed. Given the abundance of OfCPH-1 in the cuticle of Ostrinia furnacalis, this liquid-liquid phase separation process and its aging behavior could play critical roles in the formation of the cuticle. Ministry of Education (MOE) National Research Foundation (NRF) Submitted/Accepted version Q.G., J.W., Y.H., and J.Y. thank the Singapore National Research Fellowship (NRF-NRFF11-2019-0004) and the Singapore Ministry of Education (MOE) Tier 2 Grant (MOE-T2EP30220-0006). L.C., Q.Y., and T.L. thank the support from the National Natural Science Foundation of China (31871959, 31830076, and 32170502). X.-X.Z. thanks the support from the Fundamental Research Funds for the Central Universities [(DUT21RC(3)030)]. 2022-09-12T08:20:19Z 2022-09-12T08:20:19Z 2022 Journal Article Gong, Q., Chen, L., Wang, J., Yuan, F., Ma, Z., Chen, G., Huang, Y., Miao, Y., Liu, T., Zhang, X., Yang, Q. & Yu, J. (2022). Coassembly of a new insect cuticular protein and chitosan via liquid–liquid phase separation. Biomacromolecules, 23(6), 2562-2571. https://dx.doi.org/10.1021/acs.biomac.2c00261 1525-7797 https://hdl.handle.net/10356/161612 10.1021/acs.biomac.2c00261 6 23 2562 2571 en NRF-NRFF11-2019-0004 MOE-T2EP30220-0006 Biomacromolecules This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright © 2022 American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.biomac.2c00261. application/pdf |
| spellingShingle | Science::Biological sciences Chitosan Peptides and Proteins Gong, Qiuyu Chen, Lei Wang, Jining Yuan, Fenghou Ma, Zhiming Chen, Guoxin Huang, Yinjuan Miao, Yansong Liu, Tian Zhang, Xin-Xing Yang, Qing Yu, Jing Coassembly of a new insect cuticular protein and chitosan via liquid–liquid phase separation |
| title | Coassembly of a new insect cuticular protein and chitosan via liquid–liquid phase separation |
| title_full | Coassembly of a new insect cuticular protein and chitosan via liquid–liquid phase separation |
| title_fullStr | Coassembly of a new insect cuticular protein and chitosan via liquid–liquid phase separation |
| title_full_unstemmed | Coassembly of a new insect cuticular protein and chitosan via liquid–liquid phase separation |
| title_short | Coassembly of a new insect cuticular protein and chitosan via liquid–liquid phase separation |
| title_sort | coassembly of a new insect cuticular protein and chitosan via liquid liquid phase separation |
| topic | Science::Biological sciences Chitosan Peptides and Proteins |
| url | https://hdl.handle.net/10356/161612 |
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