Elucidating membrane proteomic responses of Escherichia coli to alcohol stress
Bioalcohol is a renewable and environment-friendly alternative to present day fossil fuels, which are non-renewable and greenhouse gas emitters. However, alcohol toxicity to microbial cells is the limiting factor to profitable, large-scale production of alcohol by fermentation. An in-depth unders...
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| Other Authors: | |
| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2022
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| Online Access: | https://hdl.handle.net/10356/161698 |
| _version_ | 1826124862613094400 |
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| author | Sen, Oishi |
| author2 | Staffan Kjelleberg |
| author_facet | Staffan Kjelleberg Sen, Oishi |
| author_sort | Sen, Oishi |
| collection | NTU |
| description | Bioalcohol is a renewable and environment-friendly alternative to present day
fossil fuels, which are non-renewable and greenhouse gas emitters. However,
alcohol toxicity to microbial cells is the limiting factor to profitable, large-scale
production of alcohol by fermentation. An in-depth understanding of how the
bacterial cells respond to alcohol stress by altering its membrane proteome can
provide valuable information, which might be useful in improving microbial
tolerance to alcohol and hence enable large scale production. The current study
deals with the understanding of changes in the membrane protein composition of
Escherichia coli in the presence of ethanol, butanol and hexanol. Further, the
study focusses on confirming the changes in the expression of important outer
membrane proteins under alcohol stress and predict the effect of such response
on the functional integrity of the outer membrane. |
| first_indexed | 2024-10-01T06:27:17Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/161698 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:27:17Z |
| publishDate | 2022 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1616982023-02-28T18:43:58Z Elucidating membrane proteomic responses of Escherichia coli to alcohol stress Sen, Oishi Staffan Kjelleberg School of Biological Sciences Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) Scott Rice Seviour Thomas LASKjelleberg@ntu.edu.sg Science::Biological sciences Bioalcohol is a renewable and environment-friendly alternative to present day fossil fuels, which are non-renewable and greenhouse gas emitters. However, alcohol toxicity to microbial cells is the limiting factor to profitable, large-scale production of alcohol by fermentation. An in-depth understanding of how the bacterial cells respond to alcohol stress by altering its membrane proteome can provide valuable information, which might be useful in improving microbial tolerance to alcohol and hence enable large scale production. The current study deals with the understanding of changes in the membrane protein composition of Escherichia coli in the presence of ethanol, butanol and hexanol. Further, the study focusses on confirming the changes in the expression of important outer membrane proteins under alcohol stress and predict the effect of such response on the functional integrity of the outer membrane. Doctor of Philosophy 2022-09-15T07:54:02Z 2022-09-15T07:54:02Z 2022 Thesis-Doctor of Philosophy Sen, O. (2022). Elucidating membrane proteomic responses of Escherichia coli to alcohol stress. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/161698 https://hdl.handle.net/10356/161698 10.32657/10356/161698 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Sen, Oishi Elucidating membrane proteomic responses of Escherichia coli to alcohol stress |
| title | Elucidating membrane proteomic responses of Escherichia coli to alcohol stress |
| title_full | Elucidating membrane proteomic responses of Escherichia coli to alcohol stress |
| title_fullStr | Elucidating membrane proteomic responses of Escherichia coli to alcohol stress |
| title_full_unstemmed | Elucidating membrane proteomic responses of Escherichia coli to alcohol stress |
| title_short | Elucidating membrane proteomic responses of Escherichia coli to alcohol stress |
| title_sort | elucidating membrane proteomic responses of escherichia coli to alcohol stress |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/161698 |
| work_keys_str_mv | AT senoishi elucidatingmembraneproteomicresponsesofescherichiacolitoalcoholstress |