Robust non-toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture
Non-toxic macroscale (few millimeters in diameter) cationic antibacterial beads were made for suppression of bacteria in fish culture. The beads were formed by the diffusion-driven layer by layer (dd-LBL) process using graphene oxide substrate and branched polyethyleneimine and were stabilized by po...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2022
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| Online Access: | https://hdl.handle.net/10356/161819 |
| _version_ | 1811692352981434368 |
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| author | Zhang, Penghui Carney, Tom J. Schroën, Karin Boom, Remko M. Chan-Park, Mary B. |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Zhang, Penghui Carney, Tom J. Schroën, Karin Boom, Remko M. Chan-Park, Mary B. |
| author_sort | Zhang, Penghui |
| collection | NTU |
| description | Non-toxic macroscale (few millimeters in diameter) cationic antibacterial beads were made for suppression of bacteria in fish culture. The beads were formed by the diffusion-driven layer by layer (dd-LBL) process using graphene oxide substrate and branched polyethyleneimine and were stabilized by post-assembly crosslinking process in which carboxyl groups on the graphene oxide were crosslinked with amine groups on the branched polyethyleneimine using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) chemistry. The crosslinked beads have negligible in vitro toxicity to 3T3 cells and zebrafish embryos. Further, the crosslinked beads exhibit excellent antibacterial activity, which can reach over 99.9% inactivation against Escherichia coli (E. coli) in 30 min even with ultrahigh (~ 108 CFU/mL) E. coli concentration. The antibacterial activity of the crosslinked beads can be regenerated with a simple procedure and remains high in the 10th cycle of regeneration. The crosslinked beads have much improved mechanical stability compared to the raw (uncrosslinked) beads. The highly porous structure and abundant functional groups of the crosslinked beads give them high adsorption capacity towards various forms of organic matter such as fish waste. Addition of zeolite nanoparticles during the bead formation process and subsequent chemical crosslinking with hyaluronic acid makes these beads suitable for removal of total ammonia nitrogen (TAN) while preserving the antibacterial activity and biocompatibility. |
| first_indexed | 2024-10-01T06:34:26Z |
| format | Journal Article |
| id | ntu-10356/161819 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:34:26Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1618192022-09-21T01:07:26Z Robust non-toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture Zhang, Penghui Carney, Tom J. Schroën, Karin Boom, Remko M. Chan-Park, Mary B. School of Chemical and Biomedical Engineering Lee Kong Chian School of Medicine (LKCMedicine) Institute of Molecular and Cell Biology, A*STAR NTU Food Technology Centre Centre for Antimicrobial Bioengineering Engineering::Bioengineering Aquaculture Antibacterial Performance Non-toxic macroscale (few millimeters in diameter) cationic antibacterial beads were made for suppression of bacteria in fish culture. The beads were formed by the diffusion-driven layer by layer (dd-LBL) process using graphene oxide substrate and branched polyethyleneimine and were stabilized by post-assembly crosslinking process in which carboxyl groups on the graphene oxide were crosslinked with amine groups on the branched polyethyleneimine using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) chemistry. The crosslinked beads have negligible in vitro toxicity to 3T3 cells and zebrafish embryos. Further, the crosslinked beads exhibit excellent antibacterial activity, which can reach over 99.9% inactivation against Escherichia coli (E. coli) in 30 min even with ultrahigh (~ 108 CFU/mL) E. coli concentration. The antibacterial activity of the crosslinked beads can be regenerated with a simple procedure and remains high in the 10th cycle of regeneration. The crosslinked beads have much improved mechanical stability compared to the raw (uncrosslinked) beads. The highly porous structure and abundant functional groups of the crosslinked beads give them high adsorption capacity towards various forms of organic matter such as fish waste. Addition of zeolite nanoparticles during the bead formation process and subsequent chemical crosslinking with hyaluronic acid makes these beads suitable for removal of total ammonia nitrogen (TAN) while preserving the antibacterial activity and biocompatibility. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) Ministry of Health (MOH) Nanyang Technological University This work was funded and supported by the Singapore MOE Tier 3 grants (MOE2013-T3-1-002 and MOE2018-T3-1-003), a Singapore MOH Industry Alignment Fund (NMRC/MOHIAFCAT2/ 003/2014), an A*Star Specialty Chemical grant (SERC A1786a0032), CARIE (Centre for Aquaculture Research Innovation and Education), and an NTU iFood Grant. Zhang P.H. acknowledges the support of NTU through a PhD scholarship. 2022-09-21T01:07:26Z 2022-09-21T01:07:26Z 2022 Journal Article Zhang, P., Carney, T. J., Schroën, K., Boom, R. M. & Chan-Park, M. B. (2022). Robust non-toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture. Aquaculture, 559, 738442-. https://dx.doi.org/10.1016/j.aquaculture.2022.738442 0044-8486 https://hdl.handle.net/10356/161819 10.1016/j.aquaculture.2022.738442 2-s2.0-85131416472 559 738442 en MOE2013-T3-1-002 MOE2018-T3-1-003 NMRC/MOHIAFCAT2/ 003/2014 SERC A1786a0032 Aquaculture © 2022 Elsevier B.V. All rights reserved. |
| spellingShingle | Engineering::Bioengineering Aquaculture Antibacterial Performance Zhang, Penghui Carney, Tom J. Schroën, Karin Boom, Remko M. Chan-Park, Mary B. Robust non-toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture |
| title | Robust non-toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture |
| title_full | Robust non-toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture |
| title_fullStr | Robust non-toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture |
| title_full_unstemmed | Robust non-toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture |
| title_short | Robust non-toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture |
| title_sort | robust non toxic macroscale beads with antibacterial and contaminant scavenging properties for aquaculture |
| topic | Engineering::Bioengineering Aquaculture Antibacterial Performance |
| url | https://hdl.handle.net/10356/161819 |
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