Metagenomic assembled genomes unravel purple non‑sulfur bacteria (PNSB) involved in integrating C, N, P biotransformation
Purple non‑sulfur bacteria (PNSB) based bioprocess has been developed to remove carbon, nitrogen and phosphorus from wastewater. However, the interactions of various bioconversion of carbon (C), nitrogen (N) and phosphorus (P) are not completely clear. In this study, a genome-centric metagenomic app...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/161830 |
| _version_ | 1811685707376230400 |
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| author | Wu, Dan Zhao, Zelong Zhang, Liang Wang, Li Zhou, Yan |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Wu, Dan Zhao, Zelong Zhang, Liang Wang, Li Zhou, Yan |
| author_sort | Wu, Dan |
| collection | NTU |
| description | Purple non‑sulfur bacteria (PNSB) based bioprocess has been developed to remove carbon, nitrogen and phosphorus from wastewater. However, the interactions of various bioconversion of carbon (C), nitrogen (N) and phosphorus (P) are not completely clear. In this study, a genome-centric metagenomic approach was employed to delineate the shift in microbial community structures and functional genes under light and dark conditions. Seven and 22 metagenomic assembled genomes (MAGs) were recovered from samples in light and dark conditions, accounting for a substantial portion of microbes. Under light, Rhodopseudomonas palustris promoted complex metabolic processes and interactions for C, N and P conversions. Burkholderia contaminans was discovered as new potential organisms for simultaneous C, N and P removal. Metagenomics analysis confirmed genes involved in the synthesis of glycogen, poly-β-hydroxybutyrate, poly-P, amino acids and carotenoids in R. palustris. The substrate transformation mechanisms and potential pathways were proposed according to the detected metabolites. Our findings provided insights into a new biological system with simultaneous C, N and P bioconversions, and improved the understanding of interactions among the key populations. |
| first_indexed | 2024-10-01T04:48:48Z |
| format | Journal Article |
| id | ntu-10356/161830 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:48:48Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1618302022-09-21T02:57:40Z Metagenomic assembled genomes unravel purple non‑sulfur bacteria (PNSB) involved in integrating C, N, P biotransformation Wu, Dan Zhao, Zelong Zhang, Liang Wang, Li Zhou, Yan School of Civil and Environmental Engineering Nanyang Environment and Water Research Institute Advanced Environmental Biotechnology Centre (AEBC) Engineering::Environmental engineering Biotransformation Metagenomics Purple non‑sulfur bacteria (PNSB) based bioprocess has been developed to remove carbon, nitrogen and phosphorus from wastewater. However, the interactions of various bioconversion of carbon (C), nitrogen (N) and phosphorus (P) are not completely clear. In this study, a genome-centric metagenomic approach was employed to delineate the shift in microbial community structures and functional genes under light and dark conditions. Seven and 22 metagenomic assembled genomes (MAGs) were recovered from samples in light and dark conditions, accounting for a substantial portion of microbes. Under light, Rhodopseudomonas palustris promoted complex metabolic processes and interactions for C, N and P conversions. Burkholderia contaminans was discovered as new potential organisms for simultaneous C, N and P removal. Metagenomics analysis confirmed genes involved in the synthesis of glycogen, poly-β-hydroxybutyrate, poly-P, amino acids and carotenoids in R. palustris. The substrate transformation mechanisms and potential pathways were proposed according to the detected metabolites. Our findings provided insights into a new biological system with simultaneous C, N and P bioconversions, and improved the understanding of interactions among the key populations. Agency for Science, Technology and Research (A*STAR) Nanyang Technological University The authors are grateful for the financial support provided by the Advanced Environmental Biotechnology Centre (AEBC) of Nanyang Technological University, the Research Fund for the Doctoral Program of Singapore and Interdisciplinary Graduate School of Nanyang Technological University. This work was also supported by A*STAR SFS IAF-PP grant (A20H7a0152) awarded to Yan Zhou. 2022-09-21T02:57:40Z 2022-09-21T02:57:40Z 2022 Journal Article Wu, D., Zhao, Z., Zhang, L., Wang, L. & Zhou, Y. (2022). Metagenomic assembled genomes unravel purple non‑sulfur bacteria (PNSB) involved in integrating C, N, P biotransformation. Science of the Total Environment, 830, 154591-. https://dx.doi.org/10.1016/j.scitotenv.2022.154591 0048-9697 https://hdl.handle.net/10356/161830 10.1016/j.scitotenv.2022.154591 35318065 2-s2.0-85127013971 830 154591 en A20H7a0152 Science of the Total Environment © 2022 Elsevier B.V. All rights reserved. |
| spellingShingle | Engineering::Environmental engineering Biotransformation Metagenomics Wu, Dan Zhao, Zelong Zhang, Liang Wang, Li Zhou, Yan Metagenomic assembled genomes unravel purple non‑sulfur bacteria (PNSB) involved in integrating C, N, P biotransformation |
| title | Metagenomic assembled genomes unravel purple non‑sulfur bacteria (PNSB) involved in integrating C, N, P biotransformation |
| title_full | Metagenomic assembled genomes unravel purple non‑sulfur bacteria (PNSB) involved in integrating C, N, P biotransformation |
| title_fullStr | Metagenomic assembled genomes unravel purple non‑sulfur bacteria (PNSB) involved in integrating C, N, P biotransformation |
| title_full_unstemmed | Metagenomic assembled genomes unravel purple non‑sulfur bacteria (PNSB) involved in integrating C, N, P biotransformation |
| title_short | Metagenomic assembled genomes unravel purple non‑sulfur bacteria (PNSB) involved in integrating C, N, P biotransformation |
| title_sort | metagenomic assembled genomes unravel purple non sulfur bacteria pnsb involved in integrating c n p biotransformation |
| topic | Engineering::Environmental engineering Biotransformation Metagenomics |
| url | https://hdl.handle.net/10356/161830 |
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