A renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia-reperfusion injury
Although hepatic ischemia-reperfusion injury (IRI) represents a major complication in many clinical settings, it remains a diagnostic dilemma due to its reliance on insensitive assays or invasive biopsy. The development of an activatable polymeric nanoprobe (APNSO ) for real-time in vivo near-infrar...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/162044 |
| _version_ | 1826124921421430784 |
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| author | Huang, Jiaguo Xian, Shiyun Liu, Yi Chen, Xiaona Pu, Kanyi Wang, Hangxiang |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Huang, Jiaguo Xian, Shiyun Liu, Yi Chen, Xiaona Pu, Kanyi Wang, Hangxiang |
| author_sort | Huang, Jiaguo |
| collection | NTU |
| description | Although hepatic ischemia-reperfusion injury (IRI) represents a major complication in many clinical settings, it remains a diagnostic dilemma due to its reliance on insensitive assays or invasive biopsy. The development of an activatable polymeric nanoprobe (APNSO ) for real-time in vivo near-infrared fluorescence (NIRF) imaging and urinalysis of hepatic IRI is reported here. APNSO has a backbone comprising renally clearable fluorophore fragments and self-immolative structural units. In the presence of an oxidative stress biomarker (superoxide anion, O2 •- ) during hepatic IRI, APNSO can be fluorescently activated for in vivo NIRF imaging and depolymerized to release renally clearable fluorophores for urinalysis. By virtue of its high hepatic accumulation, sensitive response toward O2 •- , and effective release of renally clearable fluorophores, APNSO -based imaging and urinalysis detect hepatic IRI at least 7 h earlier than typical clinical assays in a mouse model. This study not only provides new opportunities for noninvasive diagnosis of hepatic IRI, but also reveals guidelines for the development of optical nanosensors for early urinalysis. |
| first_indexed | 2024-10-01T06:28:16Z |
| format | Journal Article |
| id | ntu-10356/162044 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:28:16Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1620442022-10-03T01:50:05Z A renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia-reperfusion injury Huang, Jiaguo Xian, Shiyun Liu, Yi Chen, Xiaona Pu, Kanyi Wang, Hangxiang School of Chemical and Biomedical Engineering Engineering::Bioengineering Activatable Polymeric Nanoprobe Fluorescence Imaging Although hepatic ischemia-reperfusion injury (IRI) represents a major complication in many clinical settings, it remains a diagnostic dilemma due to its reliance on insensitive assays or invasive biopsy. The development of an activatable polymeric nanoprobe (APNSO ) for real-time in vivo near-infrared fluorescence (NIRF) imaging and urinalysis of hepatic IRI is reported here. APNSO has a backbone comprising renally clearable fluorophore fragments and self-immolative structural units. In the presence of an oxidative stress biomarker (superoxide anion, O2 •- ) during hepatic IRI, APNSO can be fluorescently activated for in vivo NIRF imaging and depolymerized to release renally clearable fluorophores for urinalysis. By virtue of its high hepatic accumulation, sensitive response toward O2 •- , and effective release of renally clearable fluorophores, APNSO -based imaging and urinalysis detect hepatic IRI at least 7 h earlier than typical clinical assays in a mouse model. This study not only provides new opportunities for noninvasive diagnosis of hepatic IRI, but also reveals guidelines for the development of optical nanosensors for early urinalysis. Ministry of Education (MOE) K.P. thanks Singapore Ministry of Education, Academic Research Fund Tier 1 (2019-T1-002-045, RG125/19), Academic Research Fund Tier 2 (MOE2018-T2-2-042 and MOE-T2EP30220-0010) for the financial support. J.H. acknowledges the support from the start-up funding of Sun Yat-Sen University. H.W. thanks Zhejiang Provincial Natural Science Foundation of China (LR19H160002), National Natural Science Foundation of China (82073296 and 81773193), Research Project of Jinan Microecological Biomedicine Shandong Laboratory (JNL-2022010B) for the financial support. 2022-10-03T01:50:05Z 2022-10-03T01:50:05Z 2022 Journal Article Huang, J., Xian, S., Liu, Y., Chen, X., Pu, K. & Wang, H. (2022). A renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia-reperfusion injury. Advanced Materials, 34(24), e2201357-. https://dx.doi.org/10.1002/adma.202201357 0935-9648 https://hdl.handle.net/10356/162044 10.1002/adma.202201357 35436014 2-s2.0-85129985741 24 34 e2201357 en 2019-T1-002-045 RG125/19 MOE2018-T2-2-042 MOE-T2EP30220-0010 Advanced Materials © 2022 Wiley-VCH GmbH. All rights reserved. |
| spellingShingle | Engineering::Bioengineering Activatable Polymeric Nanoprobe Fluorescence Imaging Huang, Jiaguo Xian, Shiyun Liu, Yi Chen, Xiaona Pu, Kanyi Wang, Hangxiang A renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia-reperfusion injury |
| title | A renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia-reperfusion injury |
| title_full | A renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia-reperfusion injury |
| title_fullStr | A renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia-reperfusion injury |
| title_full_unstemmed | A renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia-reperfusion injury |
| title_short | A renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia-reperfusion injury |
| title_sort | renally clearable activatable polymeric nanoprobe for early detection of hepatic ischemia reperfusion injury |
| topic | Engineering::Bioengineering Activatable Polymeric Nanoprobe Fluorescence Imaging |
| url | https://hdl.handle.net/10356/162044 |
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