An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy
Imaging to evaluate tumor-associated neutrophils (TANs) is imperative for cancer immunotherapy but remains challenging. We herein report an activatable semiconducting polymer nanoprobe (SPCy) for near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging of neutrophil elastase (NE), a biomarke...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/162212 |
| _version_ | 1811691538812502016 |
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| author | Zhang, Yan He, Shasha Xu, Cheng Jiang, Yue Miao, Qingqing Pu, Kanyi |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Zhang, Yan He, Shasha Xu, Cheng Jiang, Yue Miao, Qingqing Pu, Kanyi |
| author_sort | Zhang, Yan |
| collection | NTU |
| description | Imaging to evaluate tumor-associated neutrophils (TANs) is imperative for cancer immunotherapy but remains challenging. We herein report an activatable semiconducting polymer nanoprobe (SPCy) for near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging of neutrophil elastase (NE), a biomarker of TANs. SPCy comprises a semiconducting polymer conjugated with a hemicyanine (hemi-Cy) dye caged by a NE-cleavable peptide as the side chain. After systemic administration, SPCy passively targets the tumor and reacts with NE to "uncage" the hemi-Cy, leading to enhanced NIRF and PA signals of the hemi-Cy but unchanged signals of the SP. Such NE-activated ratiometric NIRF and enhanced PA signals of SPCy correlate with the intratumoral population of TANs. Thus, this study not only presents the first TAN-specific PA probe, but also provides a general molecular design strategy for PA imaging of other immune-related biomarkers to facilitate screening of cancer immunotherapeutics. |
| first_indexed | 2024-10-01T06:21:29Z |
| format | Journal Article |
| id | ntu-10356/162212 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:21:29Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1622122022-10-10T04:15:08Z An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy Zhang, Yan He, Shasha Xu, Cheng Jiang, Yue Miao, Qingqing Pu, Kanyi School of Chemical and Biomedical Engineering School of Physical and Mathematical Sciences Lee Kong Chian School of Medicine (LKCMedicine) Engineering::Bioengineering Science::Medicine Activatable Probe Cancer Immunotherapy Imaging to evaluate tumor-associated neutrophils (TANs) is imperative for cancer immunotherapy but remains challenging. We herein report an activatable semiconducting polymer nanoprobe (SPCy) for near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging of neutrophil elastase (NE), a biomarker of TANs. SPCy comprises a semiconducting polymer conjugated with a hemicyanine (hemi-Cy) dye caged by a NE-cleavable peptide as the side chain. After systemic administration, SPCy passively targets the tumor and reacts with NE to "uncage" the hemi-Cy, leading to enhanced NIRF and PA signals of the hemi-Cy but unchanged signals of the SP. Such NE-activated ratiometric NIRF and enhanced PA signals of SPCy correlate with the intratumoral population of TANs. Thus, this study not only presents the first TAN-specific PA probe, but also provides a general molecular design strategy for PA imaging of other immune-related biomarkers to facilitate screening of cancer immunotherapeutics. Ministry of Education (MOE) This work was supported by the National Key Research and Development Program of China (2021YFA1101500). K.P. thanks Singapore Ministry of Education, Academic Research Fund Tier 1 (2019-T1-002-045, RG125/19, RT05/20) Academic Research Fund Tier 2 (MOE2018-T2-2-042; MOE-T2EP30220-0010) for the financial support. 2022-10-10T03:56:50Z 2022-10-10T03:56:50Z 2022 Journal Article Zhang, Y., He, S., Xu, C., Jiang, Y., Miao, Q. & Pu, K. (2022). An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy. Angewandte Chemie (International Ed. in English), 61(27), e202203184-. https://dx.doi.org/10.1002/anie.202203184 1433-7851 https://hdl.handle.net/10356/162212 10.1002/anie.202203184 35385175 2-s2.0-85129374058 27 61 e202203184 en 2019-T1-002-045 RG125/19 RT05/20 MOE2018-T2-2-042 MOE-T2EP30220-0010 Angewandte Chemie (International ed. in English) © 2022 Wiley-VCH GmbH. All rights reserved. |
| spellingShingle | Engineering::Bioengineering Science::Medicine Activatable Probe Cancer Immunotherapy Zhang, Yan He, Shasha Xu, Cheng Jiang, Yue Miao, Qingqing Pu, Kanyi An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy |
| title | An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy |
| title_full | An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy |
| title_fullStr | An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy |
| title_full_unstemmed | An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy |
| title_short | An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy |
| title_sort | activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor associated neutrophils in cancer immunotherapy |
| topic | Engineering::Bioengineering Science::Medicine Activatable Probe Cancer Immunotherapy |
| url | https://hdl.handle.net/10356/162212 |
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