Fabricating tissues in situ with the controlled cellular alignments
Tissue engineering techniques have enabled to replicate the geometrical architecture of native tissues but usually fail to reproduce their exact cellular arrangements during the fabricating process, while it is critical for manufacturing physiologically relevant tissues. To address this problem, a &...
Main Authors: | , , , , , , , , , , |
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Format: | Journal Article |
Language: | English |
Published: |
2022
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Online Access: | https://hdl.handle.net/10356/162388 |
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author | He, Chuanjiang Liu, Mengxue Jiang, Deming Wu, Jianguo Qin, Chunlian Liang, Tao Wu, Pan Han, Chunmao Huang, Liquan Hsia, K. Jimmy Wang, Ping |
author2 | School of Chemical and Biomedical Engineering |
author_facet | School of Chemical and Biomedical Engineering He, Chuanjiang Liu, Mengxue Jiang, Deming Wu, Jianguo Qin, Chunlian Liang, Tao Wu, Pan Han, Chunmao Huang, Liquan Hsia, K. Jimmy Wang, Ping |
author_sort | He, Chuanjiang |
collection | NTU |
description | Tissue engineering techniques have enabled to replicate the geometrical architecture of native tissues but usually fail to reproduce their exact cellular arrangements during the fabricating process, while it is critical for manufacturing physiologically relevant tissues. To address this problem, a "sewing-like" method of controlling cellular alignment during the fabricating process is reported here. By integrating the stretching step into the fabricating process, a static mechanical environment is created which, in turn, regulates the subsequent cellular alignment, elongation, and differentiation in the generated tissues. With this method, patterned cellular constructs can be fabricated with controlled cellular alignment. Moreover, this method shows a potent capability to fabricate physiologically relevant skeletal muscle constructs in vitro by mechanically inducing myoblast fusion and maturation. As a potential clinical application, aligned myofibers are directly fabricated onto injured muscles in vivo, which repair the damaged tissues effectively. This study shows that the "sewing-like" method can produce engineered tissues with precise control of cellular arrangements and more clinically viable functionalities. |
first_indexed | 2024-10-01T07:18:52Z |
format | Journal Article |
id | ntu-10356/162388 |
institution | Nanyang Technological University |
language | English |
last_indexed | 2024-10-01T07:18:52Z |
publishDate | 2022 |
record_format | dspace |
spelling | ntu-10356/1623882022-10-17T07:44:29Z Fabricating tissues in situ with the controlled cellular alignments He, Chuanjiang Liu, Mengxue Jiang, Deming Wu, Jianguo Qin, Chunlian Liang, Tao Wu, Pan Han, Chunmao Huang, Liquan Hsia, K. Jimmy Wang, Ping School of Chemical and Biomedical Engineering School of Mechanical and Aerospace Engineering Engineering::Bioengineering Biomechanics Cellular Alignment Tissue engineering techniques have enabled to replicate the geometrical architecture of native tissues but usually fail to reproduce their exact cellular arrangements during the fabricating process, while it is critical for manufacturing physiologically relevant tissues. To address this problem, a "sewing-like" method of controlling cellular alignment during the fabricating process is reported here. By integrating the stretching step into the fabricating process, a static mechanical environment is created which, in turn, regulates the subsequent cellular alignment, elongation, and differentiation in the generated tissues. With this method, patterned cellular constructs can be fabricated with controlled cellular alignment. Moreover, this method shows a potent capability to fabricate physiologically relevant skeletal muscle constructs in vitro by mechanically inducing myoblast fusion and maturation. As a potential clinical application, aligned myofibers are directly fabricated onto injured muscles in vivo, which repair the damaged tissues effectively. This study shows that the "sewing-like" method can produce engineered tissues with precise control of cellular arrangements and more clinically viable functionalities. This study was supported by the Major International Cooperation Project of National Natural Science Foundation of China (No. 62120106004) and the Key R&D project of Ministry of Science and Technology of China (No. 2021YFB3200801). 2022-10-17T07:44:29Z 2022-10-17T07:44:29Z 2022 Journal Article He, C., Liu, M., Jiang, D., Wu, J., Qin, C., Liang, T., Wu, P., Han, C., Huang, L., Hsia, K. J. & Wang, P. (2022). Fabricating tissues in situ with the controlled cellular alignments. Advanced Healthcare Materials, 11(3), e2100934-. https://dx.doi.org/10.1002/adhm.202100934 2192-2640 https://hdl.handle.net/10356/162388 10.1002/adhm.202100934 34648692 2-s2.0-85122281815 3 11 e2100934 en Advanced Healthcare Materials © 2022 Wiley-VCH GmbH. All rights reserved. |
spellingShingle | Engineering::Bioengineering Biomechanics Cellular Alignment He, Chuanjiang Liu, Mengxue Jiang, Deming Wu, Jianguo Qin, Chunlian Liang, Tao Wu, Pan Han, Chunmao Huang, Liquan Hsia, K. Jimmy Wang, Ping Fabricating tissues in situ with the controlled cellular alignments |
title | Fabricating tissues in situ with the controlled cellular alignments |
title_full | Fabricating tissues in situ with the controlled cellular alignments |
title_fullStr | Fabricating tissues in situ with the controlled cellular alignments |
title_full_unstemmed | Fabricating tissues in situ with the controlled cellular alignments |
title_short | Fabricating tissues in situ with the controlled cellular alignments |
title_sort | fabricating tissues in situ with the controlled cellular alignments |
topic | Engineering::Bioengineering Biomechanics Cellular Alignment |
url | https://hdl.handle.net/10356/162388 |
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