A perspective on polythiophenes as conformation dependent optical reporters for label-free bioanalytics
Poly(3-alkylthiophene) (PT)-based conjugated polyelectrolytes (CPEs) constitute an important class of responsive polymers with excellent optical properties. The electrostatic interactions between PTs and target analytes trigger complexation and concomitant conformational changes of the PT backbones...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/162411 |
| _version_ | 1826112334908620800 |
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| author | Sinsinbar, Gaurav Palaniappan, Alagappan Yildiz, Umit Hakan Liedberg, Bo |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Sinsinbar, Gaurav Palaniappan, Alagappan Yildiz, Umit Hakan Liedberg, Bo |
| author_sort | Sinsinbar, Gaurav |
| collection | NTU |
| description | Poly(3-alkylthiophene) (PT)-based conjugated polyelectrolytes (CPEs) constitute an important class of responsive polymers with excellent optical properties. The electrostatic interactions between PTs and target analytes trigger complexation and concomitant conformational changes of the PT backbones that produce distinct optical responses. These conformation-induced optical responses of the PTs enable them to be utilized as reporters for detection of various analytes by employing simple UV-vis spectrophotometry or the naked eye. Numerous PTs with unique pendant groups have been synthesized to tailor their interactions with analytes such as nucleotides, ions, surfactants, proteins, and bacterial and viral pathogens. In this perspective, we discuss PT-target analyte complexation for bioanalytical applications and highlight recent advancements in point-of-care and field deployable assays. Subsequently, we highlight a few areas of critical importance for future applications of PTs as reporters, including (i) design and synthesis of specific PTs to advance the understanding of the mechanisms of interaction with target analytes, (ii) using arrays of PTs and linear discriminant analysis for selective and specific detection of target analytes, (iii) translation of conventional homogeneous solution-based assays into heterogeneous membrane-based assay formats, and finally (iv) the potential of using PT as an alternative to conjugated polymer nanoparticles and dots in bioimaging. |
| first_indexed | 2024-10-01T03:05:19Z |
| format | Journal Article |
| id | ntu-10356/162411 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:05:19Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1624112022-10-18T04:50:19Z A perspective on polythiophenes as conformation dependent optical reporters for label-free bioanalytics Sinsinbar, Gaurav Palaniappan, Alagappan Yildiz, Umit Hakan Liedberg, Bo School of Materials Science and Engineering Centre for Biomimetic Sensor Science (CBSS) Engineering::Materials Polythiophenes Conjugated Polyelectrolyte Poly(3-alkylthiophene) (PT)-based conjugated polyelectrolytes (CPEs) constitute an important class of responsive polymers with excellent optical properties. The electrostatic interactions between PTs and target analytes trigger complexation and concomitant conformational changes of the PT backbones that produce distinct optical responses. These conformation-induced optical responses of the PTs enable them to be utilized as reporters for detection of various analytes by employing simple UV-vis spectrophotometry or the naked eye. Numerous PTs with unique pendant groups have been synthesized to tailor their interactions with analytes such as nucleotides, ions, surfactants, proteins, and bacterial and viral pathogens. In this perspective, we discuss PT-target analyte complexation for bioanalytical applications and highlight recent advancements in point-of-care and field deployable assays. Subsequently, we highlight a few areas of critical importance for future applications of PTs as reporters, including (i) design and synthesis of specific PTs to advance the understanding of the mechanisms of interaction with target analytes, (ii) using arrays of PTs and linear discriminant analysis for selective and specific detection of target analytes, (iii) translation of conventional homogeneous solution-based assays into heterogeneous membrane-based assay formats, and finally (iv) the potential of using PT as an alternative to conjugated polymer nanoparticles and dots in bioimaging. Ministry of Education (MOE) Nanyang Technological University This work was funded by the Singapore Ministry of Education Academic Research Fund Tier 2 (MOE2018-T2-1-025) and the NTU-NU Institute for NanoMedicine located at the International Institute for Nanotechnology, Northwestern University, USA and the Nanyang Technological University, Singapore; Agmt10/20/14. 2022-10-18T04:50:19Z 2022-10-18T04:50:19Z 2022 Journal Article Sinsinbar, G., Palaniappan, A., Yildiz, U. H. & Liedberg, B. (2022). A perspective on polythiophenes as conformation dependent optical reporters for label-free bioanalytics. ACS Sensors, 7(3), 686-703. https://dx.doi.org/10.1021/acssensors.1c02476 2379-3694 https://hdl.handle.net/10356/162411 10.1021/acssensors.1c02476 35226461 2-s2.0-85126103255 3 7 686 703 en MOE2018-T2-1-025 ACS Sensors © 2022 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering::Materials Polythiophenes Conjugated Polyelectrolyte Sinsinbar, Gaurav Palaniappan, Alagappan Yildiz, Umit Hakan Liedberg, Bo A perspective on polythiophenes as conformation dependent optical reporters for label-free bioanalytics |
| title | A perspective on polythiophenes as conformation dependent optical reporters for label-free bioanalytics |
| title_full | A perspective on polythiophenes as conformation dependent optical reporters for label-free bioanalytics |
| title_fullStr | A perspective on polythiophenes as conformation dependent optical reporters for label-free bioanalytics |
| title_full_unstemmed | A perspective on polythiophenes as conformation dependent optical reporters for label-free bioanalytics |
| title_short | A perspective on polythiophenes as conformation dependent optical reporters for label-free bioanalytics |
| title_sort | perspective on polythiophenes as conformation dependent optical reporters for label free bioanalytics |
| topic | Engineering::Materials Polythiophenes Conjugated Polyelectrolyte |
| url | https://hdl.handle.net/10356/162411 |
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