B-box containing proteins mediate seed oil accumulation in Arabidopsis
WRINKLED1 (WRI1) transcription factor plays a pivotal role in governing plant oil biosynthesis. However, the regulatory mechanism of WRI1 remains unclear. Here, we identify a member from the B-box (BBX) family as a novel interacting partner of Arabidopsis WRI1 (AtWRI1). Currently, there is no eviden...
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| Format: | Thesis-Master by Research |
| Language: | English |
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Nanyang Technological University
2022
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| Online Access: | https://hdl.handle.net/10356/162481 |
| _version_ | 1811685959888011264 |
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| author | Noor Aqilah Noor Azlan |
| author2 | Ma Wei |
| author_facet | Ma Wei Noor Aqilah Noor Azlan |
| author_sort | Noor Aqilah Noor Azlan |
| collection | NTU |
| description | WRINKLED1 (WRI1) transcription factor plays a pivotal role in governing plant oil biosynthesis. However, the regulatory mechanism of WRI1 remains unclear. Here, we identify a member from the B-box (BBX) family as a novel interacting partner of Arabidopsis WRI1 (AtWRI1). Currently, there is no evidence showing the involvement of BBX in plant oil metabolism. This project aims to investigate the role of WRI1-BBX module in mediating plant oil biosynthesis. AtWRI1 and BBX physically interacted in the yeast-two-hybrid and the bimolecular fluorescence complementation assays. Seeds of bbx null mutants displayed reduced oil content. In contrary, transgenic lines overexpressing BBX showed increased seed oil content. Co-expression of BBX and AtWRI1 resulted in enhanced transcriptional activity of AtWRI1. Taken together, our preliminary data suggest that BBX might function as a positive regulator of AtWRI1-mediated seed oil accumulation and potentially bridge environmental signals with lipid metabolism. |
| first_indexed | 2024-10-01T04:52:49Z |
| format | Thesis-Master by Research |
| id | ntu-10356/162481 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:52:49Z |
| publishDate | 2022 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1624812023-02-28T18:39:08Z B-box containing proteins mediate seed oil accumulation in Arabidopsis Noor Aqilah Noor Azlan Ma Wei School of Biological Sciences weima@ntu.edu.sg Science::Biological sciences::Botany WRINKLED1 (WRI1) transcription factor plays a pivotal role in governing plant oil biosynthesis. However, the regulatory mechanism of WRI1 remains unclear. Here, we identify a member from the B-box (BBX) family as a novel interacting partner of Arabidopsis WRI1 (AtWRI1). Currently, there is no evidence showing the involvement of BBX in plant oil metabolism. This project aims to investigate the role of WRI1-BBX module in mediating plant oil biosynthesis. AtWRI1 and BBX physically interacted in the yeast-two-hybrid and the bimolecular fluorescence complementation assays. Seeds of bbx null mutants displayed reduced oil content. In contrary, transgenic lines overexpressing BBX showed increased seed oil content. Co-expression of BBX and AtWRI1 resulted in enhanced transcriptional activity of AtWRI1. Taken together, our preliminary data suggest that BBX might function as a positive regulator of AtWRI1-mediated seed oil accumulation and potentially bridge environmental signals with lipid metabolism. Master of Science 2022-10-25T05:26:31Z 2022-10-25T05:26:31Z 2022 Thesis-Master by Research Noor Aqilah Noor Azlan (2022). B-box containing proteins mediate seed oil accumulation in Arabidopsis. Master's thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/162481 https://hdl.handle.net/10356/162481 10.32657/10356/162481 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences::Botany Noor Aqilah Noor Azlan B-box containing proteins mediate seed oil accumulation in Arabidopsis |
| title | B-box containing proteins mediate seed oil accumulation in Arabidopsis |
| title_full | B-box containing proteins mediate seed oil accumulation in Arabidopsis |
| title_fullStr | B-box containing proteins mediate seed oil accumulation in Arabidopsis |
| title_full_unstemmed | B-box containing proteins mediate seed oil accumulation in Arabidopsis |
| title_short | B-box containing proteins mediate seed oil accumulation in Arabidopsis |
| title_sort | b box containing proteins mediate seed oil accumulation in arabidopsis |
| topic | Science::Biological sciences::Botany |
| url | https://hdl.handle.net/10356/162481 |
| work_keys_str_mv | AT nooraqilahnoorazlan bboxcontainingproteinsmediateseedoilaccumulationinarabidopsis |