Molecular chemiluminescent probes with a very long near-infrared emission wavelength for in vivo imaging
Chemiluminescence imaging is imperative for diagnostics and imaging due to its intrinsically high sensitivity. To improve in vivo detection of biomarkers, chemiluminophores that simultaneously possess near-infrared (NIR) emission and modular structures amenable to construction of activatable probes...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/162678 |
| _version_ | 1826115082327687168 |
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| author | Huang, Jingsheng Jiang, Yuyan Li, Jingchao Huang, Jiaguo Pu, Kanyi |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Huang, Jingsheng Jiang, Yuyan Li, Jingchao Huang, Jiaguo Pu, Kanyi |
| author_sort | Huang, Jingsheng |
| collection | NTU |
| description | Chemiluminescence imaging is imperative for diagnostics and imaging due to its intrinsically high sensitivity. To improve in vivo detection of biomarkers, chemiluminophores that simultaneously possess near-infrared (NIR) emission and modular structures amenable to construction of activatable probes are highly desired; however, these are rare. Herein, we report two chemiluminophores with record long NIR emission (>750 nm) via integration of dicyanomethylene-4H-benzothiopyran or dicyanomethylene-4H-benzoselenopyran with dioxetane unit. Caging of the chemiluminophores with different cleavable moieties produces NIR chemiluminescence probes (NCPs) that only produce signals upon reaction with reactive oxygen species or enzymes, for example, β-galactosidase, with a tissue-penetration depth of up to 2 cm. Thus, this study provides NIR chemiluminescence molecular scaffolds applicable for in vivo turn-on imaging of versatile biomarkers in deep tissues. |
| first_indexed | 2024-10-01T03:49:43Z |
| format | Journal Article |
| id | ntu-10356/162678 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:49:43Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1626782022-11-03T08:52:11Z Molecular chemiluminescent probes with a very long near-infrared emission wavelength for in vivo imaging Huang, Jingsheng Jiang, Yuyan Li, Jingchao Huang, Jiaguo Pu, Kanyi School of Chemical and Biomedical Engineering School of Physical and Mathematical Sciences Science::Chemistry Engineering::Bioengineering Biosensors Chemiluminescence Chemiluminescence imaging is imperative for diagnostics and imaging due to its intrinsically high sensitivity. To improve in vivo detection of biomarkers, chemiluminophores that simultaneously possess near-infrared (NIR) emission and modular structures amenable to construction of activatable probes are highly desired; however, these are rare. Herein, we report two chemiluminophores with record long NIR emission (>750 nm) via integration of dicyanomethylene-4H-benzothiopyran or dicyanomethylene-4H-benzoselenopyran with dioxetane unit. Caging of the chemiluminophores with different cleavable moieties produces NIR chemiluminescence probes (NCPs) that only produce signals upon reaction with reactive oxygen species or enzymes, for example, β-galactosidase, with a tissue-penetration depth of up to 2 cm. Thus, this study provides NIR chemiluminescence molecular scaffolds applicable for in vivo turn-on imaging of versatile biomarkers in deep tissues. Ministry of Education (MOE) Nanyang Technological University This work was supported by Nanyang Technological University (Start-up Grant: M4081627) and Singapore Ministry of Education Academic Research Fund Tier 1 (2019-T1-002-045,RG125/19) and Tier 2 (MOE2018-T2-2-042) 2022-11-03T08:52:11Z 2022-11-03T08:52:11Z 2021 Journal Article Huang, J., Jiang, Y., Li, J., Huang, J. & Pu, K. (2021). Molecular chemiluminescent probes with a very long near-infrared emission wavelength for in vivo imaging. Angewandte Chemie International Edition, 60(8), 3999-4003. https://dx.doi.org/10.1002/anie.202013531 1433-7851 https://hdl.handle.net/10356/162678 10.1002/anie.202013531 33119955 2-s2.0-85097811359 8 60 3999 4003 en M4081627 2019-T1-002-045 RG125/19 MOE2018-T2-2-042 Angewandte Chemie International Edition © 2020 Wiley-VCH GmbH. All rights reserved. |
| spellingShingle | Science::Chemistry Engineering::Bioengineering Biosensors Chemiluminescence Huang, Jingsheng Jiang, Yuyan Li, Jingchao Huang, Jiaguo Pu, Kanyi Molecular chemiluminescent probes with a very long near-infrared emission wavelength for in vivo imaging |
| title | Molecular chemiluminescent probes with a very long near-infrared emission wavelength for in vivo imaging |
| title_full | Molecular chemiluminescent probes with a very long near-infrared emission wavelength for in vivo imaging |
| title_fullStr | Molecular chemiluminescent probes with a very long near-infrared emission wavelength for in vivo imaging |
| title_full_unstemmed | Molecular chemiluminescent probes with a very long near-infrared emission wavelength for in vivo imaging |
| title_short | Molecular chemiluminescent probes with a very long near-infrared emission wavelength for in vivo imaging |
| title_sort | molecular chemiluminescent probes with a very long near infrared emission wavelength for in vivo imaging |
| topic | Science::Chemistry Engineering::Bioengineering Biosensors Chemiluminescence |
| url | https://hdl.handle.net/10356/162678 |
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