Talin and kindlin : batman and robin of Mac1 integrin activation?
Macrophage 1 (Mac1) integrin is a leukocyte-restricted adhesion molecule highly expressed on phagocytes and is essential in the development of immune responses. In a normal physiological condition, activation of Mac1 is initiated via inside-out signaling. The final common step of inside-out signalin...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/10356/16284 |
| _version_ | 1826126757569232896 |
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| author | Henni Djunaidi. |
| author2 | Tan Suet Mien |
| author_facet | Tan Suet Mien Henni Djunaidi. |
| author_sort | Henni Djunaidi. |
| collection | NTU |
| description | Macrophage 1 (Mac1) integrin is a leukocyte-restricted adhesion molecule highly expressed on phagocytes and is essential in the development of immune responses. In a normal physiological condition, activation of Mac1 is initiated via inside-out signaling. The final common step of inside-out signaling is the binding of a cytoplasmic protein talin to the integrin β cytoplasmic tail. Talin-binding unclasps the proximal region of αβ cytoplasmic tails, rendering integrin activation. Recently, another family of cytoplasmic protein kindlin is observed to bind another region along integrin β cytoplasmic tail and cooperate with talin as it boosted up the ligand affinity of platelet integrin and leukocyte function adhesion 1 (LFA-1) integrin upon activation. Thus, this study aims to clarify if kindlin-3 also serves as co-activator of Mac1 in the presence of talin. The findings in 293T transient transfection system suggested that kindlin-3 is likely to serve as the co-activator of Mac1 in the presence of talin because kindlin-3 long (K3L) enhanced the ligand-binding of Mac1 induced by talin head domain (talin-HD), but K3L alone did not activate Mac1. Any possible involvement of kindlin-3 in the cell spreading remains to be further clarified. |
| first_indexed | 2024-10-01T06:57:54Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16284 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:57:54Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/162842023-02-28T18:00:51Z Talin and kindlin : batman and robin of Mac1 integrin activation? Henni Djunaidi. Tan Suet Mien School of Biological Sciences DRNTU::Science::Biological sciences::Cytology Macrophage 1 (Mac1) integrin is a leukocyte-restricted adhesion molecule highly expressed on phagocytes and is essential in the development of immune responses. In a normal physiological condition, activation of Mac1 is initiated via inside-out signaling. The final common step of inside-out signaling is the binding of a cytoplasmic protein talin to the integrin β cytoplasmic tail. Talin-binding unclasps the proximal region of αβ cytoplasmic tails, rendering integrin activation. Recently, another family of cytoplasmic protein kindlin is observed to bind another region along integrin β cytoplasmic tail and cooperate with talin as it boosted up the ligand affinity of platelet integrin and leukocyte function adhesion 1 (LFA-1) integrin upon activation. Thus, this study aims to clarify if kindlin-3 also serves as co-activator of Mac1 in the presence of talin. The findings in 293T transient transfection system suggested that kindlin-3 is likely to serve as the co-activator of Mac1 in the presence of talin because kindlin-3 long (K3L) enhanced the ligand-binding of Mac1 induced by talin head domain (talin-HD), but K3L alone did not activate Mac1. Any possible involvement of kindlin-3 in the cell spreading remains to be further clarified. Bachelor of Science in Biological Sciences 2009-05-25T02:30:16Z 2009-05-25T02:30:16Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16284 en Nanyang Technological University 31 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Cytology Henni Djunaidi. Talin and kindlin : batman and robin of Mac1 integrin activation? |
| title | Talin and kindlin : batman and robin of Mac1 integrin activation? |
| title_full | Talin and kindlin : batman and robin of Mac1 integrin activation? |
| title_fullStr | Talin and kindlin : batman and robin of Mac1 integrin activation? |
| title_full_unstemmed | Talin and kindlin : batman and robin of Mac1 integrin activation? |
| title_short | Talin and kindlin : batman and robin of Mac1 integrin activation? |
| title_sort | talin and kindlin batman and robin of mac1 integrin activation |
| topic | DRNTU::Science::Biological sciences::Cytology |
| url | http://hdl.handle.net/10356/16284 |
| work_keys_str_mv | AT hennidjunaidi talinandkindlinbatmanandrobinofmac1integrinactivation |