Spatial organization of synaptic plasticity in cerebellar parallel fiber : Purkinje cell synapses
Not much is known about spatial patterns of Parallel fiber-Purkinje cell long-term depression (PF-PC LTD). For this reason, I visualized the distribution of PF-PC LTD in relation to the area of conjunctive stimulation by using voltage-sensitive dye imaging. During stimulation, the basic optical r...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/10356/16319 |
| _version_ | 1811691601674633216 |
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| author | Zhou, Ariyo Mitriya Wenyong |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Zhou, Ariyo Mitriya Wenyong |
| author_sort | Zhou, Ariyo Mitriya Wenyong |
| collection | NTU |
| description | Not much is known about spatial patterns of Parallel fiber-Purkinje cell long-term
depression (PF-PC LTD). For this reason, I visualized the distribution of PF-PC LTD in
relation to the area of conjunctive stimulation by using voltage-sensitive dye imaging.
During stimulation, the basic optical response was observed to have an early “spiky”
component and a slower trailing component. Following a 1 Hz train of stimuli, LTD was
induced and could be visualized as a decrease in the response elicited by subsequent
stimuli. Such LTD was restricted to the molecular layer of the cerebellum, consistent
with a decrease in transmission at the PF-PC synapse. Ratio imaging allowed me to
further characterize the spatial range of LTD. Line scan analysis revealed that the
amount of LTD generally decreased with distance from the stimulation site,
demonstrating a gradient limited by the distribution of activated PF synapses. In addition,
LTD extended for 80 μm further than the peak area of PF activation, consistent with
previously indications of the spread of LTD beyond active PF synapses. I conclude that
LTD spread is likely to be limited by 2 things: (1) the distribution of activated PF
synapses; and (2) the spread of chemical signals generated during LTD. |
| first_indexed | 2024-10-01T06:22:29Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16319 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:22:29Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/163192023-02-28T18:05:21Z Spatial organization of synaptic plasticity in cerebellar parallel fiber : Purkinje cell synapses Zhou, Ariyo Mitriya Wenyong School of Biological Sciences Duke-NUS Medical School George J. Augustine DRNTU::Science::Biological sciences::Cytology Not much is known about spatial patterns of Parallel fiber-Purkinje cell long-term depression (PF-PC LTD). For this reason, I visualized the distribution of PF-PC LTD in relation to the area of conjunctive stimulation by using voltage-sensitive dye imaging. During stimulation, the basic optical response was observed to have an early “spiky” component and a slower trailing component. Following a 1 Hz train of stimuli, LTD was induced and could be visualized as a decrease in the response elicited by subsequent stimuli. Such LTD was restricted to the molecular layer of the cerebellum, consistent with a decrease in transmission at the PF-PC synapse. Ratio imaging allowed me to further characterize the spatial range of LTD. Line scan analysis revealed that the amount of LTD generally decreased with distance from the stimulation site, demonstrating a gradient limited by the distribution of activated PF synapses. In addition, LTD extended for 80 μm further than the peak area of PF activation, consistent with previously indications of the spread of LTD beyond active PF synapses. I conclude that LTD spread is likely to be limited by 2 things: (1) the distribution of activated PF synapses; and (2) the spread of chemical signals generated during LTD. Bachelor of Science in Biological Sciences 2009-05-25T04:11:58Z 2009-05-25T04:11:58Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16319 en Nanyang Technological University 39 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Cytology Zhou, Ariyo Mitriya Wenyong Spatial organization of synaptic plasticity in cerebellar parallel fiber : Purkinje cell synapses |
| title | Spatial organization of synaptic plasticity in cerebellar parallel fiber : Purkinje cell synapses |
| title_full | Spatial organization of synaptic plasticity in cerebellar parallel fiber : Purkinje cell synapses |
| title_fullStr | Spatial organization of synaptic plasticity in cerebellar parallel fiber : Purkinje cell synapses |
| title_full_unstemmed | Spatial organization of synaptic plasticity in cerebellar parallel fiber : Purkinje cell synapses |
| title_short | Spatial organization of synaptic plasticity in cerebellar parallel fiber : Purkinje cell synapses |
| title_sort | spatial organization of synaptic plasticity in cerebellar parallel fiber purkinje cell synapses |
| topic | DRNTU::Science::Biological sciences::Cytology |
| url | http://hdl.handle.net/10356/16319 |
| work_keys_str_mv | AT zhouariyomitriyawenyong spatialorganizationofsynapticplasticityincerebellarparallelfiberpurkinjecellsynapses |