Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress
The ecological roles of microbial communities and how they interact with each other in thermal hydrolysis process (THP) assisted thermophilic anaerobic digestion (THP-AD) reactors remain largely unknown, especially under propionate stress. Two thermophilic THP-AD reactors had methane yield of 240-24...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/163320 |
| _version_ | 1824454529386545152 |
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| author | Zhang, Liang Gong, Xianzhe Chen, Zhiyi Zhou, Yan |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Zhang, Liang Gong, Xianzhe Chen, Zhiyi Zhou, Yan |
| author_sort | Zhang, Liang |
| collection | NTU |
| description | The ecological roles of microbial communities and how they interact with each other in thermal hydrolysis process (THP) assisted thermophilic anaerobic digestion (THP-AD) reactors remain largely unknown, especially under propionate stress. Two thermophilic THP-AD reactors had methane yield of 240-248 mL/g VSadded, but accumulated approximately 2000 mg/L propionate. Genome-centric metagenomics analysis showed that 68 metagenome-assembled genomes (MAGs) were recovered, 32 MAGs of which were substantially enriched. Firmicutes spp. dominated the enriched microbial community, including hydrolytic/fermentative bacteria and syntrophs. Methanogenic activities were mainly mediated by Methanosarcina sp. and Methanothermobacter spp. In addition to hydrogenotrophic methanogens, Thermodesulfovibrio sp. could also be a vital H2 scavenger, contributing to maintaining low H2 partial pressure in the bioreactors. The remarkable accumulation of propionate could be likely attributed to the weak syntrophic propionate-oxidizing activity or its absence. These findings advanced our knowledge about the mutualistic symbiosis of carbon metabolism in thermophilic THP-AD reactors. |
| first_indexed | 2025-02-19T03:23:46Z |
| format | Journal Article |
| id | ntu-10356/163320 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:23:46Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1633202022-12-02T02:49:34Z Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress Zhang, Liang Gong, Xianzhe Chen, Zhiyi Zhou, Yan School of Civil and Environmental Engineering Engineering::Civil engineering Anaerobic Digestion Methanogenesis The ecological roles of microbial communities and how they interact with each other in thermal hydrolysis process (THP) assisted thermophilic anaerobic digestion (THP-AD) reactors remain largely unknown, especially under propionate stress. Two thermophilic THP-AD reactors had methane yield of 240-248 mL/g VSadded, but accumulated approximately 2000 mg/L propionate. Genome-centric metagenomics analysis showed that 68 metagenome-assembled genomes (MAGs) were recovered, 32 MAGs of which were substantially enriched. Firmicutes spp. dominated the enriched microbial community, including hydrolytic/fermentative bacteria and syntrophs. Methanogenic activities were mainly mediated by Methanosarcina sp. and Methanothermobacter spp. In addition to hydrogenotrophic methanogens, Thermodesulfovibrio sp. could also be a vital H2 scavenger, contributing to maintaining low H2 partial pressure in the bioreactors. The remarkable accumulation of propionate could be likely attributed to the weak syntrophic propionate-oxidizing activity or its absence. These findings advanced our knowledge about the mutualistic symbiosis of carbon metabolism in thermophilic THP-AD reactors. Nanyang Technological University The authors acknowledge the financial support of Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute (NEWRI), Nanyang Technological University, the National Natural Science Foundation of China (42006134), and Shandong University Foundation for Future Scholar Plan. 2022-12-02T02:49:33Z 2022-12-02T02:49:33Z 2022 Journal Article Zhang, L., Gong, X., Chen, Z. & Zhou, Y. (2022). Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress. Bioresource Technology, 360, 127574-. https://dx.doi.org/10.1016/j.biortech.2022.127574 0960-8524 https://hdl.handle.net/10356/163320 10.1016/j.biortech.2022.127574 35792328 2-s2.0-85133931106 360 127574 en Bioresource technology © 2022 Elsevier Ltd. All rights reserved. |
| spellingShingle | Engineering::Civil engineering Anaerobic Digestion Methanogenesis Zhang, Liang Gong, Xianzhe Chen, Zhiyi Zhou, Yan Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress |
| title | Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress |
| title_full | Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress |
| title_fullStr | Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress |
| title_full_unstemmed | Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress |
| title_short | Genome-centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis-assisted thermophilic anaerobic digesters under propionate stress |
| title_sort | genome centric metagenomics analysis revealed the metabolic function of abundant microbial communities in thermal hydrolysis assisted thermophilic anaerobic digesters under propionate stress |
| topic | Engineering::Civil engineering Anaerobic Digestion Methanogenesis |
| url | https://hdl.handle.net/10356/163320 |
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