Role of synaptotagmin-7 in glucagon-like peptide (GLP-1) secretion.
Glucagon-like peptide-1 (GLP-1), produced by intestinal L cells, is an important incretin with insulinotropic properties. Recent studies have highlighted the possibilities of GLP-1 based treatment for Type 2 Diabetes Mellitus. However, molecular mechanism of GLP-1 secretion regulation remains to be...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/16350 |
| _version_ | 1826118784453181440 |
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| author | Kang, Yvonne Yu Han. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Kang, Yvonne Yu Han. |
| author_sort | Kang, Yvonne Yu Han. |
| collection | NTU |
| description | Glucagon-like peptide-1 (GLP-1), produced by intestinal L cells, is an important incretin with insulinotropic properties. Recent studies have highlighted the possibilities of GLP-1 based treatment for Type 2 Diabetes Mellitus. However, molecular mechanism of GLP-1 secretion regulation remains to be elucidated. Synaptotagmins is a family of membrane trafficking proteins consisting of 15 different isoforms. Among the different synaptotagmin isoforms, the function of synaptotagmin-1, -2 and -9 as calcium sensor in regulating exocytosis of neurotransmitter and Synaptotagmin-7 as calcium sensor in neuropeptide and hormone secretion have been well characterized. The calcium sensor role of other synaptotagmin isoforms remains to be explored. Considering Synaptotagmin-7’s function role as a calcium sensor in regulating the exocytosis of large dense core vesicles, we investigated whether synaptotagmin-7 has a function as a calcium sensor in regulating calcium-triggered GLP-1 secretion. Here, we examined the effect of synaptotagmin-7 knockdown on GLP-1 secretion using a L-cell model, GLUTag. In addition, we analysed analysis on GLP-1 secretion in synaptotagmin-7 mutant mice. Results from these studies revealed a possible role of synaptotagmin-7 as a calcium sensor in regulating GLP-1 exocytosis. |
| first_indexed | 2024-10-01T04:49:09Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16350 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:49:09Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/163502023-02-28T18:04:25Z Role of synaptotagmin-7 in glucagon-like peptide (GLP-1) secretion. Kang, Yvonne Yu Han. School of Biological Sciences Han, Weiping DRNTU::Science::Biological sciences::Molecular biology Glucagon-like peptide-1 (GLP-1), produced by intestinal L cells, is an important incretin with insulinotropic properties. Recent studies have highlighted the possibilities of GLP-1 based treatment for Type 2 Diabetes Mellitus. However, molecular mechanism of GLP-1 secretion regulation remains to be elucidated. Synaptotagmins is a family of membrane trafficking proteins consisting of 15 different isoforms. Among the different synaptotagmin isoforms, the function of synaptotagmin-1, -2 and -9 as calcium sensor in regulating exocytosis of neurotransmitter and Synaptotagmin-7 as calcium sensor in neuropeptide and hormone secretion have been well characterized. The calcium sensor role of other synaptotagmin isoforms remains to be explored. Considering Synaptotagmin-7’s function role as a calcium sensor in regulating the exocytosis of large dense core vesicles, we investigated whether synaptotagmin-7 has a function as a calcium sensor in regulating calcium-triggered GLP-1 secretion. Here, we examined the effect of synaptotagmin-7 knockdown on GLP-1 secretion using a L-cell model, GLUTag. In addition, we analysed analysis on GLP-1 secretion in synaptotagmin-7 mutant mice. Results from these studies revealed a possible role of synaptotagmin-7 as a calcium sensor in regulating GLP-1 exocytosis. Bachelor of Science in Biological Sciences 2009-05-25T07:06:42Z 2009-05-25T07:06:42Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16350 en Nanyang Technological University 30 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Molecular biology Kang, Yvonne Yu Han. Role of synaptotagmin-7 in glucagon-like peptide (GLP-1) secretion. |
| title | Role of synaptotagmin-7 in glucagon-like peptide (GLP-1) secretion. |
| title_full | Role of synaptotagmin-7 in glucagon-like peptide (GLP-1) secretion. |
| title_fullStr | Role of synaptotagmin-7 in glucagon-like peptide (GLP-1) secretion. |
| title_full_unstemmed | Role of synaptotagmin-7 in glucagon-like peptide (GLP-1) secretion. |
| title_short | Role of synaptotagmin-7 in glucagon-like peptide (GLP-1) secretion. |
| title_sort | role of synaptotagmin 7 in glucagon like peptide glp 1 secretion |
| topic | DRNTU::Science::Biological sciences::Molecular biology |
| url | http://hdl.handle.net/10356/16350 |
| work_keys_str_mv | AT kangyvonneyuhan roleofsynaptotagmin7inglucagonlikepeptideglp1secretion |