Electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush-like nanocarbon microelectrode
We present a highly efficient multichannel microfluidic electrochemical sensor integrated with an electroactive nanocarbon microelectrode for sensitive and selective detection of multiple biomarkers in different biological samples. Our results have shown that ionic liquid-assisted wet spinning follo...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://hdl.handle.net/10356/163539 |
| _version_ | 1824453899390550016 |
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| author | Xu, Yun Huang, Wei Zhang, Yan Duan, Hongwei Xiao, Fei |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Xu, Yun Huang, Wei Zhang, Yan Duan, Hongwei Xiao, Fei |
| author_sort | Xu, Yun |
| collection | NTU |
| description | We present a highly efficient multichannel microfluidic electrochemical sensor integrated with an electroactive nanocarbon microelectrode for sensitive and selective detection of multiple biomarkers in different biological samples. Our results have shown that ionic liquid-assisted wet spinning followed by tailored growth of metal-organic frameworks and pyrolysis treatment led to structural and molecular engineering of mechanically robust all-carbon microfibers for excellent electrochemical activities. The flexible bottlebrush-like nanocarbon microelectrode features a "stem" of freestanding N, B-codoped graphene fiber and high-density "bristles" of Co, N-codoped carbon nanotube arrays, leading to promoted electrocatalytic mechanism that has been substantiated by density functional theory calculations. The structural characteristics, high catalytic activities, and favorable biocompatibility of the bottlebrush nanocarbon electrodes provide opportunities for multichannel, microfluidic detection of redox-active biomolecules, including hydrogen sulfide (H2S), dopamine (DA), uric acid (UA), and ascorbic acid (AA), and have been applied to on-chip monitoring of H2S and DA released from live cancer cells or neuroblastoma cells and DA, UA, and AA in trace amounts of body fluids such as sweat, finger blood, tears, saliva, and urine, which is of great significance for clinical diagnosis and prognosis in point-of-care testing. |
| first_indexed | 2025-02-19T03:13:45Z |
| format | Journal Article |
| id | ntu-10356/163539 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:13:45Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1635392022-12-08T08:02:02Z Electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush-like nanocarbon microelectrode Xu, Yun Huang, Wei Zhang, Yan Duan, Hongwei Xiao, Fei School of Chemical and Biomedical Engineering Engineering::Chemical engineering Dopamine Nanoparticles We present a highly efficient multichannel microfluidic electrochemical sensor integrated with an electroactive nanocarbon microelectrode for sensitive and selective detection of multiple biomarkers in different biological samples. Our results have shown that ionic liquid-assisted wet spinning followed by tailored growth of metal-organic frameworks and pyrolysis treatment led to structural and molecular engineering of mechanically robust all-carbon microfibers for excellent electrochemical activities. The flexible bottlebrush-like nanocarbon microelectrode features a "stem" of freestanding N, B-codoped graphene fiber and high-density "bristles" of Co, N-codoped carbon nanotube arrays, leading to promoted electrocatalytic mechanism that has been substantiated by density functional theory calculations. The structural characteristics, high catalytic activities, and favorable biocompatibility of the bottlebrush nanocarbon electrodes provide opportunities for multichannel, microfluidic detection of redox-active biomolecules, including hydrogen sulfide (H2S), dopamine (DA), uric acid (UA), and ascorbic acid (AA), and have been applied to on-chip monitoring of H2S and DA released from live cancer cells or neuroblastoma cells and DA, UA, and AA in trace amounts of body fluids such as sweat, finger blood, tears, saliva, and urine, which is of great significance for clinical diagnosis and prognosis in point-of-care testing. This work is supported by the National Natural Science Foundation of China (project no. 21874051) and Program for HUST Academic Frontier Youth Team (2019QYTD11). 2022-12-08T08:02:02Z 2022-12-08T08:02:02Z 2022 Journal Article Xu, Y., Huang, W., Zhang, Y., Duan, H. & Xiao, F. (2022). Electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush-like nanocarbon microelectrode. Analytical Chemistry, 94(10), 4463-4473. https://dx.doi.org/10.1021/acs.analchem.1c05544 0003-2700 https://hdl.handle.net/10356/163539 10.1021/acs.analchem.1c05544 35199513 2-s2.0-85125643394 10 94 4463 4473 en Analytical Chemistry © 2022 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering::Chemical engineering Dopamine Nanoparticles Xu, Yun Huang, Wei Zhang, Yan Duan, Hongwei Xiao, Fei Electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush-like nanocarbon microelectrode |
| title | Electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush-like nanocarbon microelectrode |
| title_full | Electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush-like nanocarbon microelectrode |
| title_fullStr | Electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush-like nanocarbon microelectrode |
| title_full_unstemmed | Electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush-like nanocarbon microelectrode |
| title_short | Electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush-like nanocarbon microelectrode |
| title_sort | electrochemical microfluidic multiplexed bioanalysis by a highly active bottlebrush like nanocarbon microelectrode |
| topic | Engineering::Chemical engineering Dopamine Nanoparticles |
| url | https://hdl.handle.net/10356/163539 |
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