Biomolecular function discovery through computational protein sequence analysis.
MJ1157 and Zadh2 are 2 proteins of unknown functions. Sequence alignment analysis showed that the former is possibly involved in the thiolation of uridine of tRNA while the latter is possibly involved in countering oxidative stress in cells via its oxidoreductase domain. To this latter protein, expe...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2009
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/16363 |
| _version_ | 1826118091114807296 |
|---|---|
| author | Sameer Abdul Jalil. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Sameer Abdul Jalil. |
| author_sort | Sameer Abdul Jalil. |
| collection | NTU |
| description | MJ1157 and Zadh2 are 2 proteins of unknown functions. Sequence alignment analysis showed that the former is possibly involved in the thiolation of uridine of tRNA while the latter is possibly involved in countering oxidative stress in cells via its oxidoreductase domain. To this latter protein, experiments were done to verify this possible function. The protein was overexpressed in 293T cells and they were found, via the XTT cell viability assay, to counter H2O2-induced oxidative stress. The XTT assay showed that cells with overexpressed Zadh2 were more viable than normal cells even after the addition of the peroxide, thus showing that this protein might be used by the peroxisome to counter oxidative stress that the cell suffers. |
| first_indexed | 2024-10-01T04:38:04Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16363 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:38:04Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/163632023-02-28T17:59:58Z Biomolecular function discovery through computational protein sequence analysis. Sameer Abdul Jalil. School of Biological Sciences Sebastian Maurer-Stroh DRNTU::Science::Biological sciences::Molecular biology MJ1157 and Zadh2 are 2 proteins of unknown functions. Sequence alignment analysis showed that the former is possibly involved in the thiolation of uridine of tRNA while the latter is possibly involved in countering oxidative stress in cells via its oxidoreductase domain. To this latter protein, experiments were done to verify this possible function. The protein was overexpressed in 293T cells and they were found, via the XTT cell viability assay, to counter H2O2-induced oxidative stress. The XTT assay showed that cells with overexpressed Zadh2 were more viable than normal cells even after the addition of the peroxide, thus showing that this protein might be used by the peroxisome to counter oxidative stress that the cell suffers. Bachelor of Science in Biological Sciences 2009-05-25T08:06:44Z 2009-05-25T08:06:44Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16363 en Nanyang Technological University 27 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Molecular biology Sameer Abdul Jalil. Biomolecular function discovery through computational protein sequence analysis. |
| title | Biomolecular function discovery through computational protein sequence analysis. |
| title_full | Biomolecular function discovery through computational protein sequence analysis. |
| title_fullStr | Biomolecular function discovery through computational protein sequence analysis. |
| title_full_unstemmed | Biomolecular function discovery through computational protein sequence analysis. |
| title_short | Biomolecular function discovery through computational protein sequence analysis. |
| title_sort | biomolecular function discovery through computational protein sequence analysis |
| topic | DRNTU::Science::Biological sciences::Molecular biology |
| url | http://hdl.handle.net/10356/16363 |
| work_keys_str_mv | AT sameerabduljalil biomolecularfunctiondiscoverythroughcomputationalproteinsequenceanalysis |