Electrochemically active biofilm-enabled biosensors: current status and opportunities for biofilm engineering
Electrochemically active biofilms (EABs) are formed by electroactive bacteria capable of exchanging electrons with electrodes. EABs have been employed as bio-elements in bioelectrochemical sensors which sense analytes of interest by converting metabolic changes to easily detectable electrical signal...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/163642 |
| _version_ | 1826121605221187584 |
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| author | Hu, Yidan Han, Xi Shi, Liang Cao, Bin |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Hu, Yidan Han, Xi Shi, Liang Cao, Bin |
| author_sort | Hu, Yidan |
| collection | NTU |
| description | Electrochemically active biofilms (EABs) are formed by electroactive bacteria capable of exchanging electrons with electrodes. EABs have been employed as bio-elements in bioelectrochemical sensors which sense analytes of interest by converting metabolic changes to easily detectable electrical signals. Although EAB-enabled biosensors have shown promise in environmental applications, such as water quality monitoring, their most perceived practical applications are limited by low sensitivity, low specificity and short-term stability. Engineering EABs could be an effective strategy to improve the performance of EAB-enabled biosensors. In this review, we briefly introduce EAB with the focus on its extracellular electron transfer, development and matrix, as well as EAB-enabled biosensors including their general principle and potential applications. We then discuss key limitations of EAB-enabled biosensors and the opportunities that biofilm engineering may provide to address these limitations. |
| first_indexed | 2024-10-01T05:34:57Z |
| format | Journal Article |
| id | ntu-10356/163642 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:34:57Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1636422022-12-13T05:06:50Z Electrochemically active biofilm-enabled biosensors: current status and opportunities for biofilm engineering Hu, Yidan Han, Xi Shi, Liang Cao, Bin School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences and Engineering Engineering::Environmental engineering Biofilm Engineering Microbial Fuel Cell Electrochemically active biofilms (EABs) are formed by electroactive bacteria capable of exchanging electrons with electrodes. EABs have been employed as bio-elements in bioelectrochemical sensors which sense analytes of interest by converting metabolic changes to easily detectable electrical signals. Although EAB-enabled biosensors have shown promise in environmental applications, such as water quality monitoring, their most perceived practical applications are limited by low sensitivity, low specificity and short-term stability. Engineering EABs could be an effective strategy to improve the performance of EAB-enabled biosensors. In this review, we briefly introduce EAB with the focus on its extracellular electron transfer, development and matrix, as well as EAB-enabled biosensors including their general principle and potential applications. We then discuss key limitations of EAB-enabled biosensors and the opportunities that biofilm engineering may provide to address these limitations. Ministry of Education (MOE) National Research Foundation (NRF) This work was supported by The National Key Research and Development Program of China (2018YFA0901303) and the National Natural Science Foundation of China (NSFC91851211, 41772363). This work was also supported by the National Research Foundation and MOE Singapore under its Research Centre of Excellence Programme, Singapore Centre for Environmental Life Sciences Engineering (SCELSE) (M4330005.C70 to B.C.), Nanyang Technological University, Singapore. 2022-12-13T05:06:50Z 2022-12-13T05:06:50Z 2022 Journal Article Hu, Y., Han, X., Shi, L. & Cao, B. (2022). Electrochemically active biofilm-enabled biosensors: current status and opportunities for biofilm engineering. Electrochimica Acta, 428, 140917-. https://dx.doi.org/10.1016/j.electacta.2022.140917 0013-4686 https://hdl.handle.net/10356/163642 10.1016/j.electacta.2022.140917 2-s2.0-85135286418 428 140917 en M4330005.C70 Electrochimica Acta © 2022 Elsevier Ltd. All rights reserved. |
| spellingShingle | Engineering::Environmental engineering Biofilm Engineering Microbial Fuel Cell Hu, Yidan Han, Xi Shi, Liang Cao, Bin Electrochemically active biofilm-enabled biosensors: current status and opportunities for biofilm engineering |
| title | Electrochemically active biofilm-enabled biosensors: current status and opportunities for biofilm engineering |
| title_full | Electrochemically active biofilm-enabled biosensors: current status and opportunities for biofilm engineering |
| title_fullStr | Electrochemically active biofilm-enabled biosensors: current status and opportunities for biofilm engineering |
| title_full_unstemmed | Electrochemically active biofilm-enabled biosensors: current status and opportunities for biofilm engineering |
| title_short | Electrochemically active biofilm-enabled biosensors: current status and opportunities for biofilm engineering |
| title_sort | electrochemically active biofilm enabled biosensors current status and opportunities for biofilm engineering |
| topic | Engineering::Environmental engineering Biofilm Engineering Microbial Fuel Cell |
| url | https://hdl.handle.net/10356/163642 |
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