Deciphering the concurrence of comammox, partial denitrification and anammox in a single low-oxygen mainstream nitrogen removal reactor
One-stage anammox-based autotrophic nitrogen removal technology has attracted increasing interest to sustainable biological nitrogen removal for future wastewater treatment. However, its application in mainstream municipal wastewater treatment is still challenging due to low nitrogen and high organi...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/164157 |
| _version_ | 1826109873069228032 |
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| author | Li, Xu Wang, Gonglei Chen, Jiabo Zhou, Xin Liu, Yu |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Li, Xu Wang, Gonglei Chen, Jiabo Zhou, Xin Liu, Yu |
| author_sort | Li, Xu |
| collection | NTU |
| description | One-stage anammox-based autotrophic nitrogen removal technology has attracted increasing interest to sustainable biological nitrogen removal for future wastewater treatment. However, its application in mainstream municipal wastewater treatment is still challenging due to low nitrogen and high organics of raw wastewater. Herein, a novel Simultaneous Carbon Oxidation, partial Comammox, Denitratation and Anammox (SCOCDA) was firstly developed in a single sequencing batch biofilm reactor operated at a dissolved oxygen concentration of ∼0.5 mg/L for treating synthetic municipal wastewater (50 mg/L NH4+-N and 100-250 mg/L COD). The long-term operation showed that almost complete COD and nitrogen removal performance could be achieved at a carbon/nitrogen ratio (COD/NH4+-N) of 3-5 with the corresponding effluent total nitrogen (TN)＜5 mg/L. Microbial community and amoA-targeting amplicon sequencing analysis further verified that comammox Nitrospira spp., denitrifier Thauera and other aerobic/facultative heterotrophs could work synergistically with anammox bacteria, Candidatus Kuenenia. Moreover, nitrogen metabolic and inorganic carbon fixation pathways through the interaction between comammox and anammox were also revealed with the aid of Kyoto Encyclopedia of Genes and Genomes (KEGG). Lastly, potential application of proposed SCOCDA process was illustrated. This research sheds new light on advanced nitrogen removal towards limit of technology via the synergy of comammox and anammox. |
| first_indexed | 2024-10-01T02:25:32Z |
| format | Journal Article |
| id | ntu-10356/164157 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:25:32Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1641572023-01-06T05:53:04Z Deciphering the concurrence of comammox, partial denitrification and anammox in a single low-oxygen mainstream nitrogen removal reactor Li, Xu Wang, Gonglei Chen, Jiabo Zhou, Xin Liu, Yu School of Civil and Environmental Engineering Advanced Environmental Biotechnology Centre (AEBC) Nanyang Environment and Water Research Institute Engineering::Environmental engineering Mainstream Anammox Comammox One-stage anammox-based autotrophic nitrogen removal technology has attracted increasing interest to sustainable biological nitrogen removal for future wastewater treatment. However, its application in mainstream municipal wastewater treatment is still challenging due to low nitrogen and high organics of raw wastewater. Herein, a novel Simultaneous Carbon Oxidation, partial Comammox, Denitratation and Anammox (SCOCDA) was firstly developed in a single sequencing batch biofilm reactor operated at a dissolved oxygen concentration of ∼0.5 mg/L for treating synthetic municipal wastewater (50 mg/L NH4+-N and 100-250 mg/L COD). The long-term operation showed that almost complete COD and nitrogen removal performance could be achieved at a carbon/nitrogen ratio (COD/NH4+-N) of 3-5 with the corresponding effluent total nitrogen (TN)＜5 mg/L. Microbial community and amoA-targeting amplicon sequencing analysis further verified that comammox Nitrospira spp., denitrifier Thauera and other aerobic/facultative heterotrophs could work synergistically with anammox bacteria, Candidatus Kuenenia. Moreover, nitrogen metabolic and inorganic carbon fixation pathways through the interaction between comammox and anammox were also revealed with the aid of Kyoto Encyclopedia of Genes and Genomes (KEGG). Lastly, potential application of proposed SCOCDA process was illustrated. This research sheds new light on advanced nitrogen removal towards limit of technology via the synergy of comammox and anammox. This work was supported by the National Natural Science Foundation of China (NO. 21607111), Fundamental Research Program of Shanxi Province (NO. 20210302123198), the Opening Project of National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology and State Key Laboratory of Pollution Control, Resource Reuse Foundation, Tongji University (NO. PCRRF18011) and Innovation Project for Graduate Students of Shanxi Province (NO. RC1900001671). 2023-01-06T05:53:04Z 2023-01-06T05:53:04Z 2022 Journal Article Li, X., Wang, G., Chen, J., Zhou, X. & Liu, Y. (2022). Deciphering the concurrence of comammox, partial denitrification and anammox in a single low-oxygen mainstream nitrogen removal reactor. Chemosphere, 305, 135409-. https://dx.doi.org/10.1016/j.chemosphere.2022.135409 0045-6535 https://hdl.handle.net/10356/164157 10.1016/j.chemosphere.2022.135409 35728663 2-s2.0-85132525937 305 135409 en Chemosphere © 2022 Elsevier Ltd. All rights reserved. |
| spellingShingle | Engineering::Environmental engineering Mainstream Anammox Comammox Li, Xu Wang, Gonglei Chen, Jiabo Zhou, Xin Liu, Yu Deciphering the concurrence of comammox, partial denitrification and anammox in a single low-oxygen mainstream nitrogen removal reactor |
| title | Deciphering the concurrence of comammox, partial denitrification and anammox in a single low-oxygen mainstream nitrogen removal reactor |
| title_full | Deciphering the concurrence of comammox, partial denitrification and anammox in a single low-oxygen mainstream nitrogen removal reactor |
| title_fullStr | Deciphering the concurrence of comammox, partial denitrification and anammox in a single low-oxygen mainstream nitrogen removal reactor |
| title_full_unstemmed | Deciphering the concurrence of comammox, partial denitrification and anammox in a single low-oxygen mainstream nitrogen removal reactor |
| title_short | Deciphering the concurrence of comammox, partial denitrification and anammox in a single low-oxygen mainstream nitrogen removal reactor |
| title_sort | deciphering the concurrence of comammox partial denitrification and anammox in a single low oxygen mainstream nitrogen removal reactor |
| topic | Engineering::Environmental engineering Mainstream Anammox Comammox |
| url | https://hdl.handle.net/10356/164157 |
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