In Situ Surface-Enhanced Raman Scattering Detection of a SARS-CoV-2 Biomarker Using Flexible and Transparent Polydimethylsiloxane Films with Embedded Au Nanoplates
Coronavirus disease 2019 (COVID-19) remains an ongoing issue worldwide and continues to disrupt daily life. Transmission of infection primarily occurs through secretions when in contact with infected individuals, but more recent evidence has shown that fomites are also a source of virus transmission...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2023
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| Online Access: | https://hdl.handle.net/10356/164218 |
| _version_ | 1811679319004545024 |
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| author | Yue, Weiling Xia, Zhichao Zeng, Zhiyou Chen, Zhinan Qiao, Ling Li, Panjie He, Yi Luo, Xiaojun |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Yue, Weiling Xia, Zhichao Zeng, Zhiyou Chen, Zhinan Qiao, Ling Li, Panjie He, Yi Luo, Xiaojun |
| author_sort | Yue, Weiling |
| collection | NTU |
| description | Coronavirus disease 2019 (COVID-19) remains an ongoing issue worldwide and continues to disrupt daily life. Transmission of infection primarily occurs through secretions when in contact with infected individuals, but more recent evidence has shown that fomites are also a source of virus transmission, especially in cold-chain logistics. Traditional nucleic acid testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contamination in cold-chain logistics is time-consuming and inaccurate because of the multiplex sampling sites. Surface-enhanced Raman spectroscopy (SERS) provides a rapid, sensitive, and label-free detection route for various molecules, including viruses, through the identification of the characteristic peaks of their outer membrane proteins. In this study, we embedded arbitrarily orientated gold nanoplates (Au NPLs) in polydimethylsiloxane (PDMS) elastomer and used it as biosensor for the ultrasensitive detection of the SARS-CoV-2 spike protein in cold-chain logistics. This transparent and flexible substrate can be wrapped onto arbitrary surfaces and permits light penetration into the underlying contact surface, enabling in situ and point-of-care SERS diagnostics. The developed assay displayed high reproducibility (8.7%) and a low detection limit of 6.8 × 10-9g mL-1, indicating its potential to serve as a promising approach with increased accuracy and sensitivity for the detection of the S protein. |
| first_indexed | 2024-10-01T03:07:16Z |
| format | Journal Article |
| id | ntu-10356/164218 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:07:16Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1642182023-01-10T02:24:11Z In Situ Surface-Enhanced Raman Scattering Detection of a SARS-CoV-2 Biomarker Using Flexible and Transparent Polydimethylsiloxane Films with Embedded Au Nanoplates Yue, Weiling Xia, Zhichao Zeng, Zhiyou Chen, Zhinan Qiao, Ling Li, Panjie He, Yi Luo, Xiaojun School of Physical and Mathematical Sciences Science::Chemistry Spike Protein SERS Coronavirus disease 2019 (COVID-19) remains an ongoing issue worldwide and continues to disrupt daily life. Transmission of infection primarily occurs through secretions when in contact with infected individuals, but more recent evidence has shown that fomites are also a source of virus transmission, especially in cold-chain logistics. Traditional nucleic acid testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contamination in cold-chain logistics is time-consuming and inaccurate because of the multiplex sampling sites. Surface-enhanced Raman spectroscopy (SERS) provides a rapid, sensitive, and label-free detection route for various molecules, including viruses, through the identification of the characteristic peaks of their outer membrane proteins. In this study, we embedded arbitrarily orientated gold nanoplates (Au NPLs) in polydimethylsiloxane (PDMS) elastomer and used it as biosensor for the ultrasensitive detection of the SARS-CoV-2 spike protein in cold-chain logistics. This transparent and flexible substrate can be wrapped onto arbitrary surfaces and permits light penetration into the underlying contact surface, enabling in situ and point-of-care SERS diagnostics. The developed assay displayed high reproducibility (8.7%) and a low detection limit of 6.8 × 10-9g mL-1, indicating its potential to serve as a promising approach with increased accuracy and sensitivity for the detection of the S protein. We greatly appreciate the financial support from PhD Scientific Research Startup Foundation of Xihua University (RZ2100002826) and the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX21-0354). L.Q. thanks the China Scholarship Council (CSC) for its support (202006860052). 2023-01-10T02:24:11Z 2023-01-10T02:24:11Z 2022 Journal Article Yue, W., Xia, Z., Zeng, Z., Chen, Z., Qiao, L., Li, P., He, Y. & Luo, X. (2022). In Situ Surface-Enhanced Raman Scattering Detection of a SARS-CoV-2 Biomarker Using Flexible and Transparent Polydimethylsiloxane Films with Embedded Au Nanoplates. ACS Applied Nano Materials, 5(9), 12897-12906. https://dx.doi.org/10.1021/acsanm.2c02750 2574-0970 https://hdl.handle.net/10356/164218 10.1021/acsanm.2c02750 2-s2.0-85137307260 9 5 12897 12906 en ACS Applied Nano Materials © 2022 American Chemical Society. All rights reserved. |
| spellingShingle | Science::Chemistry Spike Protein SERS Yue, Weiling Xia, Zhichao Zeng, Zhiyou Chen, Zhinan Qiao, Ling Li, Panjie He, Yi Luo, Xiaojun In Situ Surface-Enhanced Raman Scattering Detection of a SARS-CoV-2 Biomarker Using Flexible and Transparent Polydimethylsiloxane Films with Embedded Au Nanoplates |
| title | In Situ Surface-Enhanced Raman Scattering Detection of a SARS-CoV-2 Biomarker Using Flexible and Transparent Polydimethylsiloxane Films with Embedded Au Nanoplates |
| title_full | In Situ Surface-Enhanced Raman Scattering Detection of a SARS-CoV-2 Biomarker Using Flexible and Transparent Polydimethylsiloxane Films with Embedded Au Nanoplates |
| title_fullStr | In Situ Surface-Enhanced Raman Scattering Detection of a SARS-CoV-2 Biomarker Using Flexible and Transparent Polydimethylsiloxane Films with Embedded Au Nanoplates |
| title_full_unstemmed | In Situ Surface-Enhanced Raman Scattering Detection of a SARS-CoV-2 Biomarker Using Flexible and Transparent Polydimethylsiloxane Films with Embedded Au Nanoplates |
| title_short | In Situ Surface-Enhanced Raman Scattering Detection of a SARS-CoV-2 Biomarker Using Flexible and Transparent Polydimethylsiloxane Films with Embedded Au Nanoplates |
| title_sort | in situ surface enhanced raman scattering detection of a sars cov 2 biomarker using flexible and transparent polydimethylsiloxane films with embedded au nanoplates |
| topic | Science::Chemistry Spike Protein SERS |
| url | https://hdl.handle.net/10356/164218 |
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