Structure and protein recognition of G-quadruplexes
G-rich oligonucleotides can adopt non-canonical secondary structures called G-quadruplexes (G4s) which are biologically relevant and found in applications and nanotechnology. Due to the high structural polymorphism, it is still a challenge to specifically target different G4 structures. In this stud...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2023
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| Online Access: | https://hdl.handle.net/10356/164358 |
| _version_ | 1811697698160508928 |
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| author | Ngo, Khac Huy |
| author2 | Phan Anh Tuan |
| author_facet | Phan Anh Tuan Ngo, Khac Huy |
| author_sort | Ngo, Khac Huy |
| collection | NTU |
| description | G-rich oligonucleotides can adopt non-canonical secondary structures called G-quadruplexes (G4s) which are biologically relevant and found in applications and nanotechnology. Due to the high structural polymorphism, it is still a challenge to specifically target different G4 structures. In this study, different biophysical, biochemical, and structural techniques (CD, ITC, gel electrophoresis, enzymatic assay NMR spectroscopy, X-ray crystallography) and nucleic acid/protein/peptide engineering were applied to: i) expand our understanding of G4 diversity including base stacking, interlocking, and A•G•G•G•G pentad formation; ii) study the molecular basis for G4 recognition by proteins; iii) engineer G4-binding proteins/peptides for various applications including stable cyclic peptide or engineered enzyme for G4s targeting. The findings from this study provide a better understanding of G4 structures and their recognition by proteins. |
| first_indexed | 2024-10-01T07:59:23Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/164358 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:59:23Z |
| publishDate | 2023 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1643582023-02-28T23:56:33Z Structure and protein recognition of G-quadruplexes Ngo, Khac Huy Phan Anh Tuan School of Physical and Mathematical Sciences PhanTuan@ntu.edu.sg Science::Physics G-rich oligonucleotides can adopt non-canonical secondary structures called G-quadruplexes (G4s) which are biologically relevant and found in applications and nanotechnology. Due to the high structural polymorphism, it is still a challenge to specifically target different G4 structures. In this study, different biophysical, biochemical, and structural techniques (CD, ITC, gel electrophoresis, enzymatic assay NMR spectroscopy, X-ray crystallography) and nucleic acid/protein/peptide engineering were applied to: i) expand our understanding of G4 diversity including base stacking, interlocking, and A•G•G•G•G pentad formation; ii) study the molecular basis for G4 recognition by proteins; iii) engineer G4-binding proteins/peptides for various applications including stable cyclic peptide or engineered enzyme for G4s targeting. The findings from this study provide a better understanding of G4 structures and their recognition by proteins. Doctor of Philosophy 2023-01-17T07:51:19Z 2023-01-17T07:51:19Z 2022 Thesis-Doctor of Philosophy Ngo, K. H. (2022). Structure and protein recognition of G-quadruplexes. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/164358 https://hdl.handle.net/10356/164358 10.32657/10356/164358 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Physics Ngo, Khac Huy Structure and protein recognition of G-quadruplexes |
| title | Structure and protein recognition of G-quadruplexes |
| title_full | Structure and protein recognition of G-quadruplexes |
| title_fullStr | Structure and protein recognition of G-quadruplexes |
| title_full_unstemmed | Structure and protein recognition of G-quadruplexes |
| title_short | Structure and protein recognition of G-quadruplexes |
| title_sort | structure and protein recognition of g quadruplexes |
| topic | Science::Physics |
| url | https://hdl.handle.net/10356/164358 |
| work_keys_str_mv | AT ngokhachuy structureandproteinrecognitionofgquadruplexes |