Electrospun aligned PCLl/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings
Bacterial infections and multidrug-resistant bacteria are major health burdens in wound care. Biocompatible antimicrobial agents, e.g., ε-polylysine (ε-PL), provide a broad spectrum of antibacterial properties and support dermal cell growth. Here, ε-PL was incorporated into polycaprolactone (PCL)/ge...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/164737 |
| _version_ | 1824455839937724416 |
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| author | Ghomi, Erfan Rezvani Lakshminarayanan, Rajamani Chellappan, Vijila Verma, Navin Kumar Chinnappan, Amutha Neisiany, Rasoul Esmaeely Amuthavalli, Kottaiswamy Poh, Zhi Sheng Wong, Brandon Han Siang Dubey, Nileshkumar Narayan, Roger Ramakrishna, Seeram |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Ghomi, Erfan Rezvani Lakshminarayanan, Rajamani Chellappan, Vijila Verma, Navin Kumar Chinnappan, Amutha Neisiany, Rasoul Esmaeely Amuthavalli, Kottaiswamy Poh, Zhi Sheng Wong, Brandon Han Siang Dubey, Nileshkumar Narayan, Roger Ramakrishna, Seeram |
| author_sort | Ghomi, Erfan Rezvani |
| collection | NTU |
| description | Bacterial infections and multidrug-resistant bacteria are major health burdens in wound care. Biocompatible antimicrobial agents, e.g., ε-polylysine (ε-PL), provide a broad spectrum of antibacterial properties and support dermal cell growth. Here, ε-PL was incorporated into polycaprolactone (PCL)/gelatin electrospun scaffolds collected at varying rotation speeds. Then, the samples were crosslinked using dopamine hydrochloride to provide highly proliferative dressings with broad antimicrobial activity. The morphological study showed that the electrospun wound dressings were smooth, continuous, and bead-free, with a mean diameter ranging from 267 ± 7 to 331 ± 8 nm for all random and aligned nanofibers. The fiber alignment of the electrospun PCL/gelatin scaffolds improved their tensile strength and modulus. Moreover, nanofiber mats are highly hydrophilic, which is crucial for an efficient wound dressing. The samples also demonstrated high antimicrobial properties against common wound bacterial strains, including methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus (SA), Escherichia coli (EC), Acinetobacter baumannii (AB), and Pseudomonas aeruginosa (PA). Mammalian cell proliferation and morphology assays involving primary human dermal fibroblasts (hDFs) and immortalized keratinocytes (HaCaT) showed excellent biocompatibility of the electrospun mats and remarkably aligned mats. Furthermore, aligned mats showed more cell migration than randomly oriented mats, which is desirable for more efficient wound healing. Therefore, it can be concluded that aligned PCL/gelatin mats containing ε-PL are promising for potential use in wound dressings. Graphical Abstract: [Figure not available: see fulltext.]. |
| first_indexed | 2025-02-19T03:44:35Z |
| format | Journal Article |
| id | ntu-10356/164737 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:44:35Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1647372023-02-13T05:28:31Z Electrospun aligned PCLl/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings Ghomi, Erfan Rezvani Lakshminarayanan, Rajamani Chellappan, Vijila Verma, Navin Kumar Chinnappan, Amutha Neisiany, Rasoul Esmaeely Amuthavalli, Kottaiswamy Poh, Zhi Sheng Wong, Brandon Han Siang Dubey, Nileshkumar Narayan, Roger Ramakrishna, Seeram Lee Kong Chian School of Medicine (LKCMedicine) Singapore Eye Research Institute Institute of Materials Research and Engineering, A*STAR National Skin Centre Science::Medicine Electrospinning Wound Healing Bacterial infections and multidrug-resistant bacteria are major health burdens in wound care. Biocompatible antimicrobial agents, e.g., ε-polylysine (ε-PL), provide a broad spectrum of antibacterial properties and support dermal cell growth. Here, ε-PL was incorporated into polycaprolactone (PCL)/gelatin electrospun scaffolds collected at varying rotation speeds. Then, the samples were crosslinked using dopamine hydrochloride to provide highly proliferative dressings with broad antimicrobial activity. The morphological study showed that the electrospun wound dressings were smooth, continuous, and bead-free, with a mean diameter ranging from 267 ± 7 to 331 ± 8 nm for all random and aligned nanofibers. The fiber alignment of the electrospun PCL/gelatin scaffolds improved their tensile strength and modulus. Moreover, nanofiber mats are highly hydrophilic, which is crucial for an efficient wound dressing. The samples also demonstrated high antimicrobial properties against common wound bacterial strains, including methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus (SA), Escherichia coli (EC), Acinetobacter baumannii (AB), and Pseudomonas aeruginosa (PA). Mammalian cell proliferation and morphology assays involving primary human dermal fibroblasts (hDFs) and immortalized keratinocytes (HaCaT) showed excellent biocompatibility of the electrospun mats and remarkably aligned mats. Furthermore, aligned mats showed more cell migration than randomly oriented mats, which is desirable for more efficient wound healing. Therefore, it can be concluded that aligned PCL/gelatin mats containing ε-PL are promising for potential use in wound dressings. Graphical Abstract: [Figure not available: see fulltext.]. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) Nanyang Technological University E.R.G. acknowledges the financial support from the Singapore International Graduate Award (SINGA). R.L. thanks funding support from the Duke-NUS Khoo Bridge Funding Award (Duke-NUS-KBrFA/2021/0044). N.K.V. acknowledges funding support from the Singapore Ministry of Education (MOE) under its MOE Academic Research Fund (AcRF) Tier 1 Grant (RG26/20) and the Agency for Science, Technology and Research (A*STAR) under its Wound Care Innovation for the Tropics (WCIT) Industry Alignment Fund Pre-Positioning (IAF-PP) Grant (H17/01/a0/0K9). B.H.S.W. and Z.S.P. were provided Ph.D. fellowships by HealthTech NTU and Lee Kong Chian School of Medicine, Nanyang Technological University Singapore. 2023-02-13T05:26:55Z 2023-02-13T05:26:55Z 2023 Journal Article Ghomi, E. R., Lakshminarayanan, R., Chellappan, V., Verma, N. K., Chinnappan, A., Neisiany, R. E., Amuthavalli, K., Poh, Z. S., Wong, B. H. S., Dubey, N., Narayan, R. & Ramakrishna, S. (2023). Electrospun aligned PCLl/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings. Advanced Fiber Materials, 5(1), 235-251. https://dx.doi.org/10.1007/s42765-022-00216-w 2524-7921 https://hdl.handle.net/10356/164737 10.1007/s42765-022-00216-w 2-s2.0-85139970035 1 5 235 251 en RG26/20 H17/01/a0/0K9 Advanced Fiber Materials © 2022 Donghua University, Shanghai, China. All rights reserved. |
| spellingShingle | Science::Medicine Electrospinning Wound Healing Ghomi, Erfan Rezvani Lakshminarayanan, Rajamani Chellappan, Vijila Verma, Navin Kumar Chinnappan, Amutha Neisiany, Rasoul Esmaeely Amuthavalli, Kottaiswamy Poh, Zhi Sheng Wong, Brandon Han Siang Dubey, Nileshkumar Narayan, Roger Ramakrishna, Seeram Electrospun aligned PCLl/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings |
| title | Electrospun aligned PCLl/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings |
| title_full | Electrospun aligned PCLl/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings |
| title_fullStr | Electrospun aligned PCLl/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings |
| title_full_unstemmed | Electrospun aligned PCLl/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings |
| title_short | Electrospun aligned PCLl/gelatin scaffolds mimicking the skin ECM for effective antimicrobial wound dressings |
| title_sort | electrospun aligned pcll gelatin scaffolds mimicking the skin ecm for effective antimicrobial wound dressings |
| topic | Science::Medicine Electrospinning Wound Healing |
| url | https://hdl.handle.net/10356/164737 |
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