Evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments
Chronic inflammation is considered a pressing health issue that needs resolving. Inflammatory disease such as inflammatory bowel disease requires a long-term medical regimen to prevent disease progression. Conventionally, lactoferrin is used to treat mild gastrointestinal tract and skin inflammation...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Online Access: | https://hdl.handle.net/10356/164774 |
| _version_ | 1811691098981007360 |
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| author | Pan, Yu Liu, Zhao Wang, Yijie Zhang, Linshen Chua, Niying Dai, Lei Chen, Jun Ho, Chun Loong |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Pan, Yu Liu, Zhao Wang, Yijie Zhang, Linshen Chua, Niying Dai, Lei Chen, Jun Ho, Chun Loong |
| author_sort | Pan, Yu |
| collection | NTU |
| description | Chronic inflammation is considered a pressing health issue that needs resolving. Inflammatory disease such as inflammatory bowel disease requires a long-term medical regimen to prevent disease progression. Conventionally, lactoferrin is used to treat mild gastrointestinal tract and skin inflammation. Protease-digested lactoferrin fragments often exhibit improved therapeutic properties compared to full-length lactoferrin (flHLF). However, there are no studies on the use of protease-digested lactoferrin fragments to treat inflammation. Herein, we assess the anti-inflammatory properties of engineered recombinant lactoferrin fragments (rtHLF4, rteHLF1, and rpHLF2) on non-malignant colonic fibroblast cells and colorectal cancer cells. We found that rtHLF4 is 10 times more effective to prevent inflammation compared to flHLF. These results were investigated by looking into the reactive oxygen species (ROS) production, angiogenesis activity, and cellular proliferation of the treated cells. We have demonstrated in this study the anti-inflammatory properties of the flHLF and the various lactoferrin fragments. These results complement the anti-cancer properties of these proteins that were demonstrated in an earlier study. |
| first_indexed | 2024-10-01T06:14:30Z |
| format | Journal Article |
| id | ntu-10356/164774 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:14:30Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1647742023-02-28T17:14:02Z Evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments Pan, Yu Liu, Zhao Wang, Yijie Zhang, Linshen Chua, Niying Dai, Lei Chen, Jun Ho, Chun Loong School of Biological Sciences Science::Biological sciences Lactoferrin Protease-Digested Chronic inflammation is considered a pressing health issue that needs resolving. Inflammatory disease such as inflammatory bowel disease requires a long-term medical regimen to prevent disease progression. Conventionally, lactoferrin is used to treat mild gastrointestinal tract and skin inflammation. Protease-digested lactoferrin fragments often exhibit improved therapeutic properties compared to full-length lactoferrin (flHLF). However, there are no studies on the use of protease-digested lactoferrin fragments to treat inflammation. Herein, we assess the anti-inflammatory properties of engineered recombinant lactoferrin fragments (rtHLF4, rteHLF1, and rpHLF2) on non-malignant colonic fibroblast cells and colorectal cancer cells. We found that rtHLF4 is 10 times more effective to prevent inflammation compared to flHLF. These results were investigated by looking into the reactive oxygen species (ROS) production, angiogenesis activity, and cellular proliferation of the treated cells. We have demonstrated in this study the anti-inflammatory properties of the flHLF and the various lactoferrin fragments. These results complement the anti-cancer properties of these proteins that were demonstrated in an earlier study. Published version This work was supported by the Shenzhen Special Fund for Innovation and Entrepreneurship of Overseas High-level Talents Peacock Team (KQTD20170810111314625), Shenzhen Institutes of Advanced Technology External Funds (DWKF20190001), National Natural Science Foundation of China’s Research Fund for International Young Scientists (22050410270), and Guangdong Innovative and Entrepreneurial Research Team Program (2019ZT08Y191). 2023-02-14T01:52:36Z 2023-02-14T01:52:36Z 2021 Journal Article Pan, Y., Liu, Z., Wang, Y., Zhang, L., Chua, N., Dai, L., Chen, J. & Ho, C. L. (2021). Evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments. Frontiers in Bioengineering and Biotechnology, 9, 779018-. https://dx.doi.org/10.3389/fbioe.2021.779018 2296-4185 https://hdl.handle.net/10356/164774 10.3389/fbioe.2021.779018 34917601 2-s2.0-85121353395 9 779018 en Frontiers in Bioengineering and Biotechnology © 2021 Pan, Liu, Wang, Zhang, Chua, Dai, Chen and Ho. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. application/pdf |
| spellingShingle | Science::Biological sciences Lactoferrin Protease-Digested Pan, Yu Liu, Zhao Wang, Yijie Zhang, Linshen Chua, Niying Dai, Lei Chen, Jun Ho, Chun Loong Evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments |
| title | Evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments |
| title_full | Evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments |
| title_fullStr | Evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments |
| title_full_unstemmed | Evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments |
| title_short | Evaluation of the anti-inflammatory and anti-oxidative effects of therapeutic human lactoferrin fragments |
| title_sort | evaluation of the anti inflammatory and anti oxidative effects of therapeutic human lactoferrin fragments |
| topic | Science::Biological sciences Lactoferrin Protease-Digested |
| url | https://hdl.handle.net/10356/164774 |
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