Combined pulsed electric field and microwave-assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro-waste
Coffee agro-waste is a potential source of polyphenols with antioxidant activity and application in the food and cosmetic trades. The usage of these byproducts persists as a challenge in the industrial landscape due to their high content of purported toxic substances hindering management. This study...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/164846 |
| _version_ | 1826127602989924352 |
|---|---|
| author | Macías-Garbett, Rodrigo Sosa-Hernández, Juan Eduardo Iqbal, Hafiz M. N. Contreras-Esquivel, Juan Carlos Chen, Wei Ning Melchor-Martínez, Elda M. Parra-Saldívar, Roberto |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Macías-Garbett, Rodrigo Sosa-Hernández, Juan Eduardo Iqbal, Hafiz M. N. Contreras-Esquivel, Juan Carlos Chen, Wei Ning Melchor-Martínez, Elda M. Parra-Saldívar, Roberto |
| author_sort | Macías-Garbett, Rodrigo |
| collection | NTU |
| description | Coffee agro-waste is a potential source of polyphenols with antioxidant activity and application in the food and cosmetic trades. The usage of these byproducts persists as a challenge in the industrial landscape due to their high content of purported toxic substances hindering management. This study presents a green extractive process using pulsed electric field (PEF) and microwave assisted extraction (MAE) to recover polyphenols from coffee parchment and two varieties of pulp, posing quick processing times and the use of water as the only solvent. The performance of this process with regard to the bioactivity was assessed through the Folin-Ciocalteu assay, total flavonoid content, DPPH, ABTS and FRAP antioxidant tests. The phenolic composition of the extracts was also determined through HPLC-MS and quantified through HPLC-DAD. When compared to treatment controls, PEF + MAE treated samples presented enhanced yields of total phenolic content and radical scavenging activity in all analyzed residues (Tukey test significance: 95%). The chromatographic studies reveal the presence of caffeic acid on the three analyzed by-products. The HPLC-DAD caffeic acid quantification validated that a combination of MAE + PEF treatment in yellow coffee pulp had the highest caffeic acid concentration of all studied extraction methods. |
| first_indexed | 2024-10-01T07:11:36Z |
| format | Journal Article |
| id | ntu-10356/164846 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:11:36Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1648462023-12-29T06:51:45Z Combined pulsed electric field and microwave-assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro-waste Macías-Garbett, Rodrigo Sosa-Hernández, Juan Eduardo Iqbal, Hafiz M. N. Contreras-Esquivel, Juan Carlos Chen, Wei Ning Melchor-Martínez, Elda M. Parra-Saldívar, Roberto School of Chemical and Biomedical Engineering Engineering::Bioengineering Polyphenols Microwave-Assisted Extraction Coffee agro-waste is a potential source of polyphenols with antioxidant activity and application in the food and cosmetic trades. The usage of these byproducts persists as a challenge in the industrial landscape due to their high content of purported toxic substances hindering management. This study presents a green extractive process using pulsed electric field (PEF) and microwave assisted extraction (MAE) to recover polyphenols from coffee parchment and two varieties of pulp, posing quick processing times and the use of water as the only solvent. The performance of this process with regard to the bioactivity was assessed through the Folin-Ciocalteu assay, total flavonoid content, DPPH, ABTS and FRAP antioxidant tests. The phenolic composition of the extracts was also determined through HPLC-MS and quantified through HPLC-DAD. When compared to treatment controls, PEF + MAE treated samples presented enhanced yields of total phenolic content and radical scavenging activity in all analyzed residues (Tukey test significance: 95%). The chromatographic studies reveal the presence of caffeic acid on the three analyzed by-products. The HPLC-DAD caffeic acid quantification validated that a combination of MAE + PEF treatment in yellow coffee pulp had the highest caffeic acid concentration of all studied extraction methods. Published version This work was performed at Centro de Biotecnología FEMSA of Tecnológico de Monterrey, Campus Monterrey, and the Laboratory of Glycobiotechnology of the Food Research Department, Universidad Autonoma de Coahuila, with the financial support of the Sustainable and Applied Biotechnology group through the CSIC-TEC joint project LINKB20026 “Baterias Biodegradables a partir de productos de biorefineria para comunidades de escasos recursos”. 2023-02-20T04:52:32Z 2023-02-20T04:52:32Z 2022 Journal Article Macías-Garbett, R., Sosa-Hernández, J. E., Iqbal, H. M. N., Contreras-Esquivel, J. C., Chen, W. N., Melchor-Martínez, E. M. & Parra-Saldívar, R. (2022). Combined pulsed electric field and microwave-assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro-waste. Plants, 11(18), 11182362-. https://dx.doi.org/10.3390/plants11182362 2223-7747 https://hdl.handle.net/10356/164846 10.3390/plants11182362 36145763 2-s2.0-85138643980 18 11 11182362 en Plants © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
| spellingShingle | Engineering::Bioengineering Polyphenols Microwave-Assisted Extraction Macías-Garbett, Rodrigo Sosa-Hernández, Juan Eduardo Iqbal, Hafiz M. N. Contreras-Esquivel, Juan Carlos Chen, Wei Ning Melchor-Martínez, Elda M. Parra-Saldívar, Roberto Combined pulsed electric field and microwave-assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro-waste |
| title | Combined pulsed electric field and microwave-assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro-waste |
| title_full | Combined pulsed electric field and microwave-assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro-waste |
| title_fullStr | Combined pulsed electric field and microwave-assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro-waste |
| title_full_unstemmed | Combined pulsed electric field and microwave-assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro-waste |
| title_short | Combined pulsed electric field and microwave-assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro-waste |
| title_sort | combined pulsed electric field and microwave assisted extraction as a green method for the recovery of antioxidant compounds with electroactive potential from coffee agro waste |
| topic | Engineering::Bioengineering Polyphenols Microwave-Assisted Extraction |
| url | https://hdl.handle.net/10356/164846 |
| work_keys_str_mv | AT maciasgarbettrodrigo combinedpulsedelectricfieldandmicrowaveassistedextractionasagreenmethodfortherecoveryofantioxidantcompoundswithelectroactivepotentialfromcoffeeagrowaste AT sosahernandezjuaneduardo combinedpulsedelectricfieldandmicrowaveassistedextractionasagreenmethodfortherecoveryofantioxidantcompoundswithelectroactivepotentialfromcoffeeagrowaste AT iqbalhafizmn combinedpulsedelectricfieldandmicrowaveassistedextractionasagreenmethodfortherecoveryofantioxidantcompoundswithelectroactivepotentialfromcoffeeagrowaste AT contrerasesquiveljuancarlos combinedpulsedelectricfieldandmicrowaveassistedextractionasagreenmethodfortherecoveryofantioxidantcompoundswithelectroactivepotentialfromcoffeeagrowaste AT chenweining combinedpulsedelectricfieldandmicrowaveassistedextractionasagreenmethodfortherecoveryofantioxidantcompoundswithelectroactivepotentialfromcoffeeagrowaste AT melchormartinezeldam combinedpulsedelectricfieldandmicrowaveassistedextractionasagreenmethodfortherecoveryofantioxidantcompoundswithelectroactivepotentialfromcoffeeagrowaste AT parrasaldivarroberto combinedpulsedelectricfieldandmicrowaveassistedextractionasagreenmethodfortherecoveryofantioxidantcompoundswithelectroactivepotentialfromcoffeeagrowaste |