Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium
Bats are reservoir hosts for various zoonotic viruses with pandemdic potential in humans and livestock. In vitro systems for studying bat host-pathogen interactions are of significant interest. Here, we establish protocols to generate bat airway organoids (AOs) and airway epithelial cells differenti...
| Main Authors: | , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/164848 |
| _version_ | 1824455614542118912 |
|---|---|
| author | Chan, Louisa L. Y. Gamage, Akshamal M. Tan, Chee Wah Tan, Kai Sen Liu, Jing Tay, Douglas Jie Wen Foo, Randy Jee Hiang Rénia, Laurent Wang, De Yun Wang, Lin-Fa |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Chan, Louisa L. Y. Gamage, Akshamal M. Tan, Chee Wah Tan, Kai Sen Liu, Jing Tay, Douglas Jie Wen Foo, Randy Jee Hiang Rénia, Laurent Wang, De Yun Wang, Lin-Fa |
| author_sort | Chan, Louisa L. Y. |
| collection | NTU |
| description | Bats are reservoir hosts for various zoonotic viruses with pandemdic potential in humans and livestock. In vitro systems for studying bat host-pathogen interactions are of significant interest. Here, we establish protocols to generate bat airway organoids (AOs) and airway epithelial cells differentiated at the air-liquid interface (ALI-AECs) from tracheal tissues of the cave-nectar bat Eonycteris spelaea. In particular, we describe steps which enable laboratories that do not have access to live bats to perform extended experimental work upon procuring an initial batch of bat primary airway tissue. Complete mucociliary differentiation required treatment with IL-13. E. spelaea ALI-AECs supported productive infection with PRV3M, an orthoreovirus for which Pteropodid bats are considered the reservoir species. However, these ALI-AECs did not support SARS-CoV-2 infection, despite E. spelaea ACE2 receptor being capable of mediating SARS-CoV-2 spike pseudovirus entry. This work provides critical model systems for assessing bat species-specific virus susceptibility and the reservoir likelihood for emerging infectious agents. |
| first_indexed | 2025-02-19T03:41:00Z |
| format | Journal Article |
| id | ntu-10356/164848 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:41:00Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1648482023-03-05T16:55:12Z Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium Chan, Louisa L. Y. Gamage, Akshamal M. Tan, Chee Wah Tan, Kai Sen Liu, Jing Tay, Douglas Jie Wen Foo, Randy Jee Hiang Rénia, Laurent Wang, De Yun Wang, Lin-Fa Lee Kong Chian School of Medicine (LKCMedicine) A*STAR Infectious Diseases Labs Science::Medicine Bat Chiroptera Bats are reservoir hosts for various zoonotic viruses with pandemdic potential in humans and livestock. In vitro systems for studying bat host-pathogen interactions are of significant interest. Here, we establish protocols to generate bat airway organoids (AOs) and airway epithelial cells differentiated at the air-liquid interface (ALI-AECs) from tracheal tissues of the cave-nectar bat Eonycteris spelaea. In particular, we describe steps which enable laboratories that do not have access to live bats to perform extended experimental work upon procuring an initial batch of bat primary airway tissue. Complete mucociliary differentiation required treatment with IL-13. E. spelaea ALI-AECs supported productive infection with PRV3M, an orthoreovirus for which Pteropodid bats are considered the reservoir species. However, these ALI-AECs did not support SARS-CoV-2 infection, despite E. spelaea ACE2 receptor being capable of mediating SARS-CoV-2 spike pseudovirus entry. This work provides critical model systems for assessing bat species-specific virus susceptibility and the reservoir likelihood for emerging infectious agents. Ministry of Education (MOE) Nanyang Technological University National Medical Research Council (NMRC) National Research Foundation (NRF) Published version This research was funded in part by Singapore National Research Foundation (NRF2012NRF-CRP001-056, NRF2016NRF-NSFC002-013), the Singapore National Medical Research Council of Singapore (MOH-COV-ID19RF2-0001, MOH-COVID19RF-003, MOH-OFIRG19-MAY-0011, MOH-OFIRG10NOV-0050) and the Ministry of Education Singapore (MOE2019-T2-2-130); Singapore National Medical Research Council Centre Grant Program – Diabetes, Tuberculosis and Neuroscience No.CGAug16M009; the NUS Reimagine Research Grant, Singapore; and the Nanyang Technological University Lee Kong Chian School of Medicine LEARN grant (021914-00001).LLYC is supported by the Lee Kong Chian School of Medi-cine, Nanyang Technological University under the Dean’s Postdoctoral Fellowship and the Wong Peng Onn Fellow-ship (002823-00001). 2023-02-20T05:32:51Z 2023-02-20T05:32:51Z 2023 Journal Article Chan, L. L. Y., Gamage, A. M., Tan, C. W., Tan, K. S., Liu, J., Tay, D. J. W., Foo, R. J. H., Rénia, L., Wang, D. Y. & Wang, L. (2023). Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium. Emerging Microbes & Infections, 12(1), e2148561-. https://dx.doi.org/10.1080/22221751.2022.2148561 2222-1751 https://hdl.handle.net/10356/164848 10.1080/22221751.2022.2148561 36440480 2-s2.0-85144094299 1 12 e2148561 en NRF2012NRF-CRP001-056 NRF2016NRF-NSFC002-013 MOH-COV-ID19RF2-0001 MOH-COVID19RF-003 MOH-OFIRG19-MAY-0011 MOH-OFIRG10NOV-0050 MOE2019-T2-2-130 CGAug16M009 021914-00001 002823-00001 Emerging Microbes & Infections © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. application/pdf |
| spellingShingle | Science::Medicine Bat Chiroptera Chan, Louisa L. Y. Gamage, Akshamal M. Tan, Chee Wah Tan, Kai Sen Liu, Jing Tay, Douglas Jie Wen Foo, Randy Jee Hiang Rénia, Laurent Wang, De Yun Wang, Lin-Fa Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium |
| title | Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium |
| title_full | Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium |
| title_fullStr | Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium |
| title_full_unstemmed | Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium |
| title_short | Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium |
| title_sort | generation of self replicating airway organoids from the cave nectar bat eonycteris spelaea as a model system for studying host pathogen interactions in the bat airway epithelium |
| topic | Science::Medicine Bat Chiroptera |
| url | https://hdl.handle.net/10356/164848 |
| work_keys_str_mv | AT chanlouisaly generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium AT gamageakshamalm generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium AT tancheewah generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium AT tankaisen generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium AT liujing generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium AT taydouglasjiewen generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium AT foorandyjeehiang generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium AT renialaurent generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium AT wangdeyun generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium AT wanglinfa generationofselfreplicatingairwayorganoidsfromthecavenectarbateonycterisspelaeaasamodelsystemforstudyinghostpathogeninteractionsinthebatairwayepithelium |