Transient complexity of E. Coli lipidome is explained by fatty acyl synthesis and cyclopropanation
In the case of many bacteria, such as Escherichia coli, the composition of lipid molecules, termed the lipidome, temporally adapts to different environmental conditions and thus modifies membrane properties to permit growth and survival. Details of the relationship between the environment and lipido...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Online Access: | https://hdl.handle.net/10356/164851 |
| _version_ | 1826122785147060224 |
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| author | Berezhnoy, Nikolay V. Cazenave-Gassiot, Amaury Gao, Liang Foo, Juat Chin Ji, Shanshan Regina, Viduthalai Rasheedkhan Yap, Peggy Pui Khee Wenk, Markus R. Kjelleberg, Staffan Seviour, Thomas William Hinks, Jamie |
| author2 | Singapore Centre for Environmental Life Sciences and Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences and Engineering Berezhnoy, Nikolay V. Cazenave-Gassiot, Amaury Gao, Liang Foo, Juat Chin Ji, Shanshan Regina, Viduthalai Rasheedkhan Yap, Peggy Pui Khee Wenk, Markus R. Kjelleberg, Staffan Seviour, Thomas William Hinks, Jamie |
| author_sort | Berezhnoy, Nikolay V. |
| collection | NTU |
| description | In the case of many bacteria, such as Escherichia coli, the composition of lipid molecules, termed the lipidome, temporally adapts to different environmental conditions and thus modifies membrane properties to permit growth and survival. Details of the relationship between the environment and lipidome composition are lacking, particularly for growing cultures under either favourable or under stress conditions. Here, we highlight compositional lipidome changes by describing the dynamics of molecular species throughout culture-growth phases. We show a steady cyclopropanation of fatty acyl chains, which acts as a driver for lipid diversity. There is a bias for the cyclopropanation of shorter fatty acyl chains (FA 16:1) over longer ones (FA 18:1), which likely reflects a thermodynamic phenomenon. Additionally, we observe a nearly two-fold increase in saturated fatty acyl chains in response to the presence of ampicillin and chloramphenicol, with consequences for membrane fluidity and elasticity, and ultimately bacterial stress tolerance. Our study provides the detailed quantitative lipidome composition of three E. coli strains across culture-growth phases and at the level of the fatty acyl chains and provides a general reference for phospholipid composition changes in response to perturbations. Thus, lipidome diversity is largely transient and the consequence of lipid synthesis and cyclopropanation. |
| first_indexed | 2024-10-01T05:53:53Z |
| format | Journal Article |
| id | ntu-10356/164851 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:53:53Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1648512023-02-25T15:31:46Z Transient complexity of E. Coli lipidome is explained by fatty acyl synthesis and cyclopropanation Berezhnoy, Nikolay V. Cazenave-Gassiot, Amaury Gao, Liang Foo, Juat Chin Ji, Shanshan Regina, Viduthalai Rasheedkhan Yap, Peggy Pui Khee Wenk, Markus R. Kjelleberg, Staffan Seviour, Thomas William Hinks, Jamie Singapore Centre for Environmental Life Sciences and Engineering Engineering::Bioengineering Lipidomics Cyclopropanation In the case of many bacteria, such as Escherichia coli, the composition of lipid molecules, termed the lipidome, temporally adapts to different environmental conditions and thus modifies membrane properties to permit growth and survival. Details of the relationship between the environment and lipidome composition are lacking, particularly for growing cultures under either favourable or under stress conditions. Here, we highlight compositional lipidome changes by describing the dynamics of molecular species throughout culture-growth phases. We show a steady cyclopropanation of fatty acyl chains, which acts as a driver for lipid diversity. There is a bias for the cyclopropanation of shorter fatty acyl chains (FA 16:1) over longer ones (FA 18:1), which likely reflects a thermodynamic phenomenon. Additionally, we observe a nearly two-fold increase in saturated fatty acyl chains in response to the presence of ampicillin and chloramphenicol, with consequences for membrane fluidity and elasticity, and ultimately bacterial stress tolerance. Our study provides the detailed quantitative lipidome composition of three E. coli strains across culture-growth phases and at the level of the fatty acyl chains and provides a general reference for phospholipid composition changes in response to perturbations. Thus, lipidome diversity is largely transient and the consequence of lipid synthesis and cyclopropanation. Ministry of Education (MOE) Nanyang Technological University National Research Foundation (NRF) Published version This work was funded by grant number MOE2016-T2-1-148 under the Singapore MoE AcRF program. SCELSE is funded by Singapore’s National Research Foundation, the Ministry of Education, NTU, and the National University of Singapore (NUS), and is hosted by NTU in partnership with NUS. 2023-02-20T06:17:56Z 2023-02-20T06:17:56Z 2022 Journal Article Berezhnoy, N. V., Cazenave-Gassiot, A., Gao, L., Foo, J. C., Ji, S., Regina, V. R., Yap, P. P. K., Wenk, M. R., Kjelleberg, S., Seviour, T. W. & Hinks, J. (2022). Transient complexity of E. Coli lipidome is explained by fatty acyl synthesis and cyclopropanation. Metabolites, 12(9), 12090784-. https://dx.doi.org/10.3390/metabo12090784 2218-1989 https://hdl.handle.net/10356/164851 10.3390/metabo12090784 36144187 2-s2.0-85138667872 9 12 12090784 en MOE2016-T2-1-148 Metabolites © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
| spellingShingle | Engineering::Bioengineering Lipidomics Cyclopropanation Berezhnoy, Nikolay V. Cazenave-Gassiot, Amaury Gao, Liang Foo, Juat Chin Ji, Shanshan Regina, Viduthalai Rasheedkhan Yap, Peggy Pui Khee Wenk, Markus R. Kjelleberg, Staffan Seviour, Thomas William Hinks, Jamie Transient complexity of E. Coli lipidome is explained by fatty acyl synthesis and cyclopropanation |
| title | Transient complexity of E. Coli lipidome is explained by fatty acyl synthesis and cyclopropanation |
| title_full | Transient complexity of E. Coli lipidome is explained by fatty acyl synthesis and cyclopropanation |
| title_fullStr | Transient complexity of E. Coli lipidome is explained by fatty acyl synthesis and cyclopropanation |
| title_full_unstemmed | Transient complexity of E. Coli lipidome is explained by fatty acyl synthesis and cyclopropanation |
| title_short | Transient complexity of E. Coli lipidome is explained by fatty acyl synthesis and cyclopropanation |
| title_sort | transient complexity of e coli lipidome is explained by fatty acyl synthesis and cyclopropanation |
| topic | Engineering::Bioengineering Lipidomics Cyclopropanation |
| url | https://hdl.handle.net/10356/164851 |
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