Line-scanning SD-OCT for in-vivo, non-contact, volumetric, cellular resolution imaging of the human cornea and limbus
In-vivo, non-contact, volumetric imaging of the cellular and sub-cellular structure of the human cornea and limbus with optical coherence tomography (OCT) is challenging due to involuntary eye motion that introduces both motion artifacts and blur in the OCT images. Here we present the design of a li...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/165087 |
| _version_ | 1811686317639073792 |
|---|---|
| author | Han, Le Tan, Bingyao Hosseinaee, Zohreh Chen, Lin Kun Hileeto, Denise Bizheva, Kostadinka |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Han, Le Tan, Bingyao Hosseinaee, Zohreh Chen, Lin Kun Hileeto, Denise Bizheva, Kostadinka |
| author_sort | Han, Le |
| collection | NTU |
| description | In-vivo, non-contact, volumetric imaging of the cellular and sub-cellular structure of the human cornea and limbus with optical coherence tomography (OCT) is challenging due to involuntary eye motion that introduces both motion artifacts and blur in the OCT images. Here we present the design of a line-scanning (LS) spectral-domain (SD) optical coherence tomography system that combines 2 × 3 × 1.7 µm (x, y, z) resolution in biological tissue with an image acquisition rate of ∼2,500 fps, and demonstrate its ability to image in-vivo and without contact with the tissue surface, the cellular structure of the human anterior segment tissues. Volumetric LS-SD-OCT images acquired over a field-of-view (FOV) of 0.7 mm × 1.4 mm reveal fine morphological details in the healthy human cornea, such as epithelial and endothelial cells, sub-basal nerves, as well as the cellular structure of the limbal crypts, the palisades of Vogt (POVs) and the blood microvasculature of the human limbus. LS-SD-OCT is a promising technology that can assist ophthalmologists with the early diagnostics and optimal treatment planning of ocular diseases affecting the human anterior eye. |
| first_indexed | 2024-10-01T04:58:30Z |
| format | Journal Article |
| id | ntu-10356/165087 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:58:30Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1650872023-12-29T06:48:31Z Line-scanning SD-OCT for in-vivo, non-contact, volumetric, cellular resolution imaging of the human cornea and limbus Han, Le Tan, Bingyao Hosseinaee, Zohreh Chen, Lin Kun Hileeto, Denise Bizheva, Kostadinka School of Chemical and Biomedical Engineering Singapore National Eye Center SERI-NTU Advanced Ocular Engineering (STANCE) Engineering::Bioengineering Cellular Resolution Resolution Imaging In-vivo, non-contact, volumetric imaging of the cellular and sub-cellular structure of the human cornea and limbus with optical coherence tomography (OCT) is challenging due to involuntary eye motion that introduces both motion artifacts and blur in the OCT images. Here we present the design of a line-scanning (LS) spectral-domain (SD) optical coherence tomography system that combines 2 × 3 × 1.7 µm (x, y, z) resolution in biological tissue with an image acquisition rate of ∼2,500 fps, and demonstrate its ability to image in-vivo and without contact with the tissue surface, the cellular structure of the human anterior segment tissues. Volumetric LS-SD-OCT images acquired over a field-of-view (FOV) of 0.7 mm × 1.4 mm reveal fine morphological details in the healthy human cornea, such as epithelial and endothelial cells, sub-basal nerves, as well as the cellular structure of the limbal crypts, the palisades of Vogt (POVs) and the blood microvasculature of the human limbus. LS-SD-OCT is a promising technology that can assist ophthalmologists with the early diagnostics and optimal treatment planning of ocular diseases affecting the human anterior eye. Published version Canada First Research Excellence Fund; Canadian Institutes of Health Research (446387); Natural Sciences and Engineering Research Council of Canada (312037). 2023-03-13T02:10:22Z 2023-03-13T02:10:22Z 2022 Journal Article Han, L., Tan, B., Hosseinaee, Z., Chen, L. K., Hileeto, D. & Bizheva, K. (2022). Line-scanning SD-OCT for in-vivo, non-contact, volumetric, cellular resolution imaging of the human cornea and limbus. Biomedical Optics Express, 13(7), 4007-4020. https://dx.doi.org/10.1364/BOE.465916 2156-7085 https://hdl.handle.net/10356/165087 10.1364/BOE.465916 35991928 2-s2.0-85132541093 7 13 4007 4020 en Biomedical Optics Express © 2022 Optica Publishing Group under the terms of the OPG Open Access Publishing Agreement. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved. application/pdf |
| spellingShingle | Engineering::Bioengineering Cellular Resolution Resolution Imaging Han, Le Tan, Bingyao Hosseinaee, Zohreh Chen, Lin Kun Hileeto, Denise Bizheva, Kostadinka Line-scanning SD-OCT for in-vivo, non-contact, volumetric, cellular resolution imaging of the human cornea and limbus |
| title | Line-scanning SD-OCT for in-vivo, non-contact, volumetric, cellular resolution imaging of the human cornea and limbus |
| title_full | Line-scanning SD-OCT for in-vivo, non-contact, volumetric, cellular resolution imaging of the human cornea and limbus |
| title_fullStr | Line-scanning SD-OCT for in-vivo, non-contact, volumetric, cellular resolution imaging of the human cornea and limbus |
| title_full_unstemmed | Line-scanning SD-OCT for in-vivo, non-contact, volumetric, cellular resolution imaging of the human cornea and limbus |
| title_short | Line-scanning SD-OCT for in-vivo, non-contact, volumetric, cellular resolution imaging of the human cornea and limbus |
| title_sort | line scanning sd oct for in vivo non contact volumetric cellular resolution imaging of the human cornea and limbus |
| topic | Engineering::Bioengineering Cellular Resolution Resolution Imaging |
| url | https://hdl.handle.net/10356/165087 |
| work_keys_str_mv | AT hanle linescanningsdoctforinvivononcontactvolumetriccellularresolutionimagingofthehumancorneaandlimbus AT tanbingyao linescanningsdoctforinvivononcontactvolumetriccellularresolutionimagingofthehumancorneaandlimbus AT hosseinaeezohreh linescanningsdoctforinvivononcontactvolumetriccellularresolutionimagingofthehumancorneaandlimbus AT chenlinkun linescanningsdoctforinvivononcontactvolumetriccellularresolutionimagingofthehumancorneaandlimbus AT hileetodenise linescanningsdoctforinvivononcontactvolumetriccellularresolutionimagingofthehumancorneaandlimbus AT bizhevakostadinka linescanningsdoctforinvivononcontactvolumetriccellularresolutionimagingofthehumancorneaandlimbus |