| Summary: | In the present work, three-dimensionally (3D) ordered macroporous glucose oxidase/conducting polymer nanocomposite film was synthesized by using the inverted colloidal polystyrene crystal template technique and in-situ copolymerization method. The structure of the pore can be precisely controlled by the calibrated relation between film thickness and deposition time. The in-situ copolymerization method allows proteins distributing uniformly inside conducting polymer film with high loading amount, which was confirmed by confocal microscopy. The 3D macroporous conducting polymer film also provides a good microenvironment for retaining the nature structure of the entrapped enzymes, as verified by attenuated total reflection Fourier-transform infrared spectroscopy. More importantly, direct electron transfer of proteins in the 3D nanocomposite film was observed, which provides the potential applications in third-generation biosensor.
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