Controlled 3D ordered protein/conducting polymer nanocomposite film and its direct electrochemistry.
In the present work, three-dimensionally (3D) ordered macroporous glucose oxidase/conducting polymer nanocomposite film was synthesized by using the inverted colloidal polystyrene crystal template technique and in-situ copolymerization method. The structure of the pore can be precisely controlled by...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/10356/16509 |
| _version_ | 1826114627788865536 |
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| author | Sui, Ning. |
| author2 | Li Changming |
| author_facet | Li Changming Sui, Ning. |
| author_sort | Sui, Ning. |
| collection | NTU |
| description | In the present work, three-dimensionally (3D) ordered macroporous glucose oxidase/conducting polymer nanocomposite film was synthesized by using the inverted colloidal polystyrene crystal template technique and in-situ copolymerization method. The structure of the pore can be precisely controlled by the calibrated relation between film thickness and deposition time. The in-situ copolymerization method allows proteins distributing uniformly inside conducting polymer film with high loading amount, which was confirmed by confocal microscopy. The 3D macroporous conducting polymer film also provides a good microenvironment for retaining the nature structure of the entrapped enzymes, as verified by attenuated total reflection Fourier-transform infrared spectroscopy. More importantly, direct electron transfer of proteins in the 3D nanocomposite film was observed, which provides the potential applications in third-generation biosensor. |
| first_indexed | 2024-10-01T03:42:14Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16509 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:42:14Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/165092023-03-03T15:33:51Z Controlled 3D ordered protein/conducting polymer nanocomposite film and its direct electrochemistry. Sui, Ning. Li Changming School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Polymers and polymer manufacture In the present work, three-dimensionally (3D) ordered macroporous glucose oxidase/conducting polymer nanocomposite film was synthesized by using the inverted colloidal polystyrene crystal template technique and in-situ copolymerization method. The structure of the pore can be precisely controlled by the calibrated relation between film thickness and deposition time. The in-situ copolymerization method allows proteins distributing uniformly inside conducting polymer film with high loading amount, which was confirmed by confocal microscopy. The 3D macroporous conducting polymer film also provides a good microenvironment for retaining the nature structure of the entrapped enzymes, as verified by attenuated total reflection Fourier-transform infrared spectroscopy. More importantly, direct electron transfer of proteins in the 3D nanocomposite film was observed, which provides the potential applications in third-generation biosensor. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-26T08:58:03Z 2009-05-26T08:58:03Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16509 en Nanyang Technological University 80 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Polymers and polymer manufacture Sui, Ning. Controlled 3D ordered protein/conducting polymer nanocomposite film and its direct electrochemistry. |
| title | Controlled 3D ordered protein/conducting polymer nanocomposite film and its direct electrochemistry. |
| title_full | Controlled 3D ordered protein/conducting polymer nanocomposite film and its direct electrochemistry. |
| title_fullStr | Controlled 3D ordered protein/conducting polymer nanocomposite film and its direct electrochemistry. |
| title_full_unstemmed | Controlled 3D ordered protein/conducting polymer nanocomposite film and its direct electrochemistry. |
| title_short | Controlled 3D ordered protein/conducting polymer nanocomposite film and its direct electrochemistry. |
| title_sort | controlled 3d ordered protein conducting polymer nanocomposite film and its direct electrochemistry |
| topic | DRNTU::Engineering::Chemical engineering::Polymers and polymer manufacture |
| url | http://hdl.handle.net/10356/16509 |
| work_keys_str_mv | AT suining controlled3dorderedproteinconductingpolymernanocompositefilmanditsdirectelectrochemistry |