Aeromonas dhakensis: clinical isolates with high carbapenem resistance
Aeromonas dhakensis is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical (n = 94) and non-clinical (n = 23...
| Päätekijät: | , , , , , |
|---|---|
| Muut tekijät: | |
| Aineistotyyppi: | Journal Article |
| Kieli: | English |
| Julkaistu: |
2023
|
| Aiheet: | |
| Linkit: | https://hdl.handle.net/10356/165207 |
| _version_ | 1826128445122281472 |
|---|---|
| author | Puah, Suat Moi Khor, Wei Ching Aung, Kyaw Thu Lau, Vicky Tien Tien Puthucheary, S. D. Chua, Kek Heng |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Puah, Suat Moi Khor, Wei Ching Aung, Kyaw Thu Lau, Vicky Tien Tien Puthucheary, S. D. Chua, Kek Heng |
| author_sort | Puah, Suat Moi |
| collection | NTU |
| description | Aeromonas dhakensis is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical (n = 94) and non-clinical (n = 23) isolates with dehydrated MicroScan microdilution. Carbapenem resistant isolates were further screened for genes related to carbapenem resistance using molecular assay. The isolates exhibited resistance to imipenem (76.9%), doripenem (62.4%), meropenem (41.9%), trimethoprim/sulfamethoxazole (11.1%), cefotaxime (8.5%), ceftazidime (6%), cefepime (1.7%) and aztreonam (0.9%), whereas all isolates were susceptible to amikacin. Clinical isolates showed significant association with resistance to doripenem, imipenem and meropenem compared to non-clinical isolates. These blacphA were detected in clinical isolates with resistance phenotypes: doripenem (67.2%, 45/67), imipenem (65.9%, 54/82) and meropenem (65.2%, 30/46). Our findings showed that the MicroScan microdilution method is suitable for the detection of carbapenem resistance in both clinical (48.9-87.2%) and non-clinical (4.3-13.0%) isolates. This study revealed that A. dhakensis isolates had relatively high carbapenem resistance, which may lead to potential treatment failure. Continued monitoring of aquatic sources with a larger sample size should be carried out to provide further insights. |
| first_indexed | 2024-10-01T07:25:04Z |
| format | Journal Article |
| id | ntu-10356/165207 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:25:04Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1652072023-03-20T15:31:39Z Aeromonas dhakensis: clinical isolates with high carbapenem resistance Puah, Suat Moi Khor, Wei Ching Aung, Kyaw Thu Lau, Vicky Tien Tien Puthucheary, S. D. Chua, Kek Heng School of Physical and Mathematical Sciences Singapore Food Agency Science::Biological sciences Aeromonas Dhakensis Carbapenem Aeromonas dhakensis is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical (n = 94) and non-clinical (n = 23) isolates with dehydrated MicroScan microdilution. Carbapenem resistant isolates were further screened for genes related to carbapenem resistance using molecular assay. The isolates exhibited resistance to imipenem (76.9%), doripenem (62.4%), meropenem (41.9%), trimethoprim/sulfamethoxazole (11.1%), cefotaxime (8.5%), ceftazidime (6%), cefepime (1.7%) and aztreonam (0.9%), whereas all isolates were susceptible to amikacin. Clinical isolates showed significant association with resistance to doripenem, imipenem and meropenem compared to non-clinical isolates. These blacphA were detected in clinical isolates with resistance phenotypes: doripenem (67.2%, 45/67), imipenem (65.9%, 54/82) and meropenem (65.2%, 30/46). Our findings showed that the MicroScan microdilution method is suitable for the detection of carbapenem resistance in both clinical (48.9-87.2%) and non-clinical (4.3-13.0%) isolates. This study revealed that A. dhakensis isolates had relatively high carbapenem resistance, which may lead to potential treatment failure. Continued monitoring of aquatic sources with a larger sample size should be carried out to provide further insights. Published version 2023-03-20T05:42:41Z 2023-03-20T05:42:41Z 2022 Journal Article Puah, S. M., Khor, W. C., Aung, K. T., Lau, V. T. T., Puthucheary, S. D. & Chua, K. H. (2022). Aeromonas dhakensis: clinical isolates with high carbapenem resistance. Pathogens, 11(8), 833-. https://dx.doi.org/10.3390/pathogens11080833 2076-0817 https://hdl.handle.net/10356/165207 10.3390/pathogens11080833 35894056 2-s2.0-85137555872 8 11 833 en Pathogens © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
| spellingShingle | Science::Biological sciences Aeromonas Dhakensis Carbapenem Puah, Suat Moi Khor, Wei Ching Aung, Kyaw Thu Lau, Vicky Tien Tien Puthucheary, S. D. Chua, Kek Heng Aeromonas dhakensis: clinical isolates with high carbapenem resistance |
| title | Aeromonas dhakensis: clinical isolates with high carbapenem resistance |
| title_full | Aeromonas dhakensis: clinical isolates with high carbapenem resistance |
| title_fullStr | Aeromonas dhakensis: clinical isolates with high carbapenem resistance |
| title_full_unstemmed | Aeromonas dhakensis: clinical isolates with high carbapenem resistance |
| title_short | Aeromonas dhakensis: clinical isolates with high carbapenem resistance |
| title_sort | aeromonas dhakensis clinical isolates with high carbapenem resistance |
| topic | Science::Biological sciences Aeromonas Dhakensis Carbapenem |
| url | https://hdl.handle.net/10356/165207 |
| work_keys_str_mv | AT puahsuatmoi aeromonasdhakensisclinicalisolateswithhighcarbapenemresistance AT khorweiching aeromonasdhakensisclinicalisolateswithhighcarbapenemresistance AT aungkyawthu aeromonasdhakensisclinicalisolateswithhighcarbapenemresistance AT lauvickytientien aeromonasdhakensisclinicalisolateswithhighcarbapenemresistance AT puthuchearysd aeromonasdhakensisclinicalisolateswithhighcarbapenemresistance AT chuakekheng aeromonasdhakensisclinicalisolateswithhighcarbapenemresistance |