Effects of Neu3 on the dynamics of flotillin 1 dependent endocytosis
Hog cells were transfected with Neu3-EGFP (Enhanced green fluorescent protein), flotillin 1-EGFP or Neu3-HA and flotillin 1-EGFP to test the effect that Neu3 has on the dynamics of flotillin 1 dependent endocytosis. Cells that were transfected with Neu3-HA and flotillin 1-EGFP showed lower lateral a...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/10356/16574 |
| _version_ | 1826129388391890944 |
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| author | Huang, Yongxue. |
| author2 | Chen Peng |
| author_facet | Chen Peng Huang, Yongxue. |
| author_sort | Huang, Yongxue. |
| collection | NTU |
| description | Hog cells were transfected with Neu3-EGFP (Enhanced green fluorescent protein), flotillin 1-EGFP or Neu3-HA and flotillin 1-EGFP to test the effect that Neu3 has on the dynamics of flotillin 1 dependent endocytosis. Cells that were transfected with Neu3-HA and flotillin 1-EGFP showed lower lateral average speeds and longer dwell time, implying overall lower average speed compared to those transfected with flotillin 1 only. Also, they showed lower vesicular movement in general as evident from their lower CDLong values over time compared to flotillin 1 transfected cells. They also have a different movement as evident from the different CDLong, CDArea, CDshort and aspect ratio. The average budding rate in cells transfected with Neu3-HA and flotillin 1-EGFP is also slower than cells transfected with flotillin 1 only. |
| first_indexed | 2024-10-01T07:39:47Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16574 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:39:47Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/165742023-03-03T15:39:37Z Effects of Neu3 on the dynamics of flotillin 1 dependent endocytosis Huang, Yongxue. Chen Peng School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology Hog cells were transfected with Neu3-EGFP (Enhanced green fluorescent protein), flotillin 1-EGFP or Neu3-HA and flotillin 1-EGFP to test the effect that Neu3 has on the dynamics of flotillin 1 dependent endocytosis. Cells that were transfected with Neu3-HA and flotillin 1-EGFP showed lower lateral average speeds and longer dwell time, implying overall lower average speed compared to those transfected with flotillin 1 only. Also, they showed lower vesicular movement in general as evident from their lower CDLong values over time compared to flotillin 1 transfected cells. They also have a different movement as evident from the different CDLong, CDArea, CDshort and aspect ratio. The average budding rate in cells transfected with Neu3-HA and flotillin 1-EGFP is also slower than cells transfected with flotillin 1 only. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-27T04:09:42Z 2009-05-27T04:09:42Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16574 en Nanyang Technological University 53 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biotechnology Huang, Yongxue. Effects of Neu3 on the dynamics of flotillin 1 dependent endocytosis |
| title | Effects of Neu3 on the dynamics of flotillin 1 dependent endocytosis |
| title_full | Effects of Neu3 on the dynamics of flotillin 1 dependent endocytosis |
| title_fullStr | Effects of Neu3 on the dynamics of flotillin 1 dependent endocytosis |
| title_full_unstemmed | Effects of Neu3 on the dynamics of flotillin 1 dependent endocytosis |
| title_short | Effects of Neu3 on the dynamics of flotillin 1 dependent endocytosis |
| title_sort | effects of neu3 on the dynamics of flotillin 1 dependent endocytosis |
| topic | DRNTU::Engineering::Chemical engineering::Biotechnology |
| url | http://hdl.handle.net/10356/16574 |
| work_keys_str_mv | AT huangyongxue effectsofneu3onthedynamicsofflotillin1dependentendocytosis |