Understanding viruses and their relationship with anaerobic methanotrophs (ANME) in active and inactive states in cold seeps
Cold seeps are unique environments that are home to organisms like anaerobic methanotrophs (ANME) and vast viral diversity. Despite the importance of ANME’s methane consumption to the global methane budget, not much is known about factors affecting its methane consumption in cold seeps, such as viru...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2023
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| Online Access: | https://hdl.handle.net/10356/165761 |
| _version_ | 1811688611264856064 |
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| author | Lim, Evelyn |
| author2 | Federico Lauro |
| author_facet | Federico Lauro Lim, Evelyn |
| author_sort | Lim, Evelyn |
| collection | NTU |
| description | Cold seeps are unique environments that are home to organisms like anaerobic methanotrophs (ANME) and vast viral diversity. Despite the importance of ANME’s methane consumption to the global methane budget, not much is known about factors affecting its methane consumption in cold seeps, such as viruses and dormancy. This project aims to understand viruses and their relationship with ANME in dormant and active states in cold seeps. Metagenomic analysis was done to observe the differences in lysogeny rates, proteins, and phylogenetic relationships of ANME viruses across cold seeps and ANME groups. Slurry incubation was then done to observe the response of viral populations to ANME exiting long-term dormancy.
From the metagenomic analysis, rate of lysogeny was observed to differ by site but not ANME group. Protein similarity in viruses was found to be influenced by extraction site and associated ANME groups and indications that viruses across different locations have similar replication processes was observed. Location and ANME group do not appear to have significant influence on the phylogenetic relationship between viruses as well. On the other hand, no clear conclusion on the response of viral populations to ANME exiting long-term dormancy. |
| first_indexed | 2024-10-01T05:34:57Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/165761 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:34:57Z |
| publishDate | 2023 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1657612023-04-10T15:32:08Z Understanding viruses and their relationship with anaerobic methanotrophs (ANME) in active and inactive states in cold seeps Lim, Evelyn Federico Lauro Asian School of the Environment University of California, Los Angeles (UCLA) Tina Treude Benjamin Knowles FLauro@ntu.edu.sg Science::Biological sciences::Microbiology Science::Biological sciences::Genetics Cold seeps are unique environments that are home to organisms like anaerobic methanotrophs (ANME) and vast viral diversity. Despite the importance of ANME’s methane consumption to the global methane budget, not much is known about factors affecting its methane consumption in cold seeps, such as viruses and dormancy. This project aims to understand viruses and their relationship with ANME in dormant and active states in cold seeps. Metagenomic analysis was done to observe the differences in lysogeny rates, proteins, and phylogenetic relationships of ANME viruses across cold seeps and ANME groups. Slurry incubation was then done to observe the response of viral populations to ANME exiting long-term dormancy. From the metagenomic analysis, rate of lysogeny was observed to differ by site but not ANME group. Protein similarity in viruses was found to be influenced by extraction site and associated ANME groups and indications that viruses across different locations have similar replication processes was observed. Location and ANME group do not appear to have significant influence on the phylogenetic relationship between viruses as well. On the other hand, no clear conclusion on the response of viral populations to ANME exiting long-term dormancy. Bachelor of Science in Environmental Earth Systems Science 2023-04-10T03:16:12Z 2023-04-10T03:16:12Z 2023 Final Year Project (FYP) Lim, E. (2023). Understanding viruses and their relationship with anaerobic methanotrophs (ANME) in active and inactive states in cold seeps. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/165761 https://hdl.handle.net/10356/165761 en application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences::Microbiology Science::Biological sciences::Genetics Lim, Evelyn Understanding viruses and their relationship with anaerobic methanotrophs (ANME) in active and inactive states in cold seeps |
| title | Understanding viruses and their relationship with anaerobic methanotrophs (ANME) in active and inactive states in cold seeps |
| title_full | Understanding viruses and their relationship with anaerobic methanotrophs (ANME) in active and inactive states in cold seeps |
| title_fullStr | Understanding viruses and their relationship with anaerobic methanotrophs (ANME) in active and inactive states in cold seeps |
| title_full_unstemmed | Understanding viruses and their relationship with anaerobic methanotrophs (ANME) in active and inactive states in cold seeps |
| title_short | Understanding viruses and their relationship with anaerobic methanotrophs (ANME) in active and inactive states in cold seeps |
| title_sort | understanding viruses and their relationship with anaerobic methanotrophs anme in active and inactive states in cold seeps |
| topic | Science::Biological sciences::Microbiology Science::Biological sciences::Genetics |
| url | https://hdl.handle.net/10356/165761 |
| work_keys_str_mv | AT limevelyn understandingvirusesandtheirrelationshipwithanaerobicmethanotrophsanmeinactiveandinactivestatesincoldseeps |