Receptor mediated adhesion under external stimuli.
Poly (N-isopropylacrylamide) (PIPAAm) is a natural thermoresponsive polymer (TRP) that is widely used in tissue engineering and drug delivery [5-14]. Studies have found that PIPAAm can change its hydrophilicity by altering temperature. At temperatures above its lower critical solution temperature...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/10356/16583 |
| _version_ | 1811681949699276800 |
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| author | Wong, Trina Hui San. |
| author2 | Chan Vincent |
| author_facet | Chan Vincent Wong, Trina Hui San. |
| author_sort | Wong, Trina Hui San. |
| collection | NTU |
| description | Poly (N-isopropylacrylamide) (PIPAAm) is a natural thermoresponsive polymer (TRP)
that is widely used in tissue engineering and drug delivery [5-14]. Studies have found that
PIPAAm can change its hydrophilicity by altering temperature. At temperatures above its
lower critical solution temperature (LCST) of 32°C [7, 12], PIPAAm changes its
conformation from expanded coil to a condensed globule; water molecules leaves the
polymer [6-14] and hence displaying hydrophobic character. At this condition, cell
adhesion is favourable; cells will adhere to the surface, spread and multiply in numbers
[7, 10]. As the surface is cooled below its LCST, the polymer becomes hydrated and
displays a hydrophilic character. Cells do not like this environment and they will reduce
their contact area with the surface by becoming more spherical in shape and eventually
get detached from the surface. With this intrinsic characteristic of PIPAAm, cells can be
extracted from the surface via alterations in temperature without the use of enzymes [6-
10, 12, 14]. This can prevent damage to the cell surface proteins as well as the cell itself
[7, 10, 14]. This study has successfully shown the evolution of cell de-adhesion on
PIPAAm surface under confocal reflection interference and contrast microscopy (CRICM)
and immunostaining and fluorescence microscopy. |
| first_indexed | 2024-10-01T03:49:04Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16583 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:49:04Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/165832023-03-03T15:34:03Z Receptor mediated adhesion under external stimuli. Wong, Trina Hui San. Chan Vincent School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology Poly (N-isopropylacrylamide) (PIPAAm) is a natural thermoresponsive polymer (TRP) that is widely used in tissue engineering and drug delivery [5-14]. Studies have found that PIPAAm can change its hydrophilicity by altering temperature. At temperatures above its lower critical solution temperature (LCST) of 32°C [7, 12], PIPAAm changes its conformation from expanded coil to a condensed globule; water molecules leaves the polymer [6-14] and hence displaying hydrophobic character. At this condition, cell adhesion is favourable; cells will adhere to the surface, spread and multiply in numbers [7, 10]. As the surface is cooled below its LCST, the polymer becomes hydrated and displays a hydrophilic character. Cells do not like this environment and they will reduce their contact area with the surface by becoming more spherical in shape and eventually get detached from the surface. With this intrinsic characteristic of PIPAAm, cells can be extracted from the surface via alterations in temperature without the use of enzymes [6- 10, 12, 14]. This can prevent damage to the cell surface proteins as well as the cell itself [7, 10, 14]. This study has successfully shown the evolution of cell de-adhesion on PIPAAm surface under confocal reflection interference and contrast microscopy (CRICM) and immunostaining and fluorescence microscopy. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-27T04:32:59Z 2009-05-27T04:32:59Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16583 en Nanyang Technological University 43 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biotechnology Wong, Trina Hui San. Receptor mediated adhesion under external stimuli. |
| title | Receptor mediated adhesion under external stimuli. |
| title_full | Receptor mediated adhesion under external stimuli. |
| title_fullStr | Receptor mediated adhesion under external stimuli. |
| title_full_unstemmed | Receptor mediated adhesion under external stimuli. |
| title_short | Receptor mediated adhesion under external stimuli. |
| title_sort | receptor mediated adhesion under external stimuli |
| topic | DRNTU::Engineering::Chemical engineering::Biotechnology |
| url | http://hdl.handle.net/10356/16583 |
| work_keys_str_mv | AT wongtrinahuisan receptormediatedadhesionunderexternalstimuli |