Tissue engineering small diameter blood vessels : fabrication of porous scaffold.
The interest in tissue engineered small diameter blood vessels was sparked off by the need for cardiovascular grafts in surgeries. This project aims to fabricate a porous scaffold to allow the diffusion of nutrients and growth factors in a co-culture of smooth muscle and endothelial cells found in...
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| Beste egile batzuk: | |
| Formatua: | Final Year Project (FYP) |
| Hizkuntza: | English |
| Argitaratua: |
2009
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| Sarrera elektronikoa: | http://hdl.handle.net/10356/16601 |
| _version_ | 1826116974579548160 |
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| author | Lin, Annette Luhe. |
| author2 | Chan Bee Eng, Mary |
| author_facet | Chan Bee Eng, Mary Lin, Annette Luhe. |
| author_sort | Lin, Annette Luhe. |
| collection | NTU |
| description | The interest in tissue engineered small diameter blood vessels was sparked off by
the need for cardiovascular grafts in surgeries. This project aims to fabricate a porous scaffold to allow the diffusion of nutrients and growth factors in a co-culture of smooth muscle and endothelial cells found in blood vessels. PCLLGA, a previously developed polymer, has good elasticity and mechanical properties but the solid material impedes the diffusion of macromolecules necessary for co-culture. Co-culture is important to determine the viability of the scaffold for clinical applications as a vascular graft. As the porous scaffold will be used for micro-patterning, salt leaching cannot be used to create pores as the pores produced are bigger than the micro-channels that will be used. Ideally,pores of around 5 to 10 μm are desired. However, to our knowledge, no one has successfully developed pores of a few microns in a photo-elastomeric polymer. In this project, a method was successfully developed to produce a porous tubular scaffold of about 20% porosity with solid PEG and PCLLGA. |
| first_indexed | 2024-10-01T04:20:11Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16601 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:20:11Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/166012023-03-03T15:34:49Z Tissue engineering small diameter blood vessels : fabrication of porous scaffold. Lin, Annette Luhe. Chan Bee Eng, Mary School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology The interest in tissue engineered small diameter blood vessels was sparked off by the need for cardiovascular grafts in surgeries. This project aims to fabricate a porous scaffold to allow the diffusion of nutrients and growth factors in a co-culture of smooth muscle and endothelial cells found in blood vessels. PCLLGA, a previously developed polymer, has good elasticity and mechanical properties but the solid material impedes the diffusion of macromolecules necessary for co-culture. Co-culture is important to determine the viability of the scaffold for clinical applications as a vascular graft. As the porous scaffold will be used for micro-patterning, salt leaching cannot be used to create pores as the pores produced are bigger than the micro-channels that will be used. Ideally,pores of around 5 to 10 μm are desired. However, to our knowledge, no one has successfully developed pores of a few microns in a photo-elastomeric polymer. In this project, a method was successfully developed to produce a porous tubular scaffold of about 20% porosity with solid PEG and PCLLGA. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-27T06:20:07Z 2009-05-27T06:20:07Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16601 en Nanyang Technological University 69 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biotechnology Lin, Annette Luhe. Tissue engineering small diameter blood vessels : fabrication of porous scaffold. |
| title | Tissue engineering small diameter blood vessels : fabrication of porous scaffold. |
| title_full | Tissue engineering small diameter blood vessels : fabrication of porous scaffold. |
| title_fullStr | Tissue engineering small diameter blood vessels : fabrication of porous scaffold. |
| title_full_unstemmed | Tissue engineering small diameter blood vessels : fabrication of porous scaffold. |
| title_short | Tissue engineering small diameter blood vessels : fabrication of porous scaffold. |
| title_sort | tissue engineering small diameter blood vessels fabrication of porous scaffold |
| topic | DRNTU::Engineering::Chemical engineering::Biotechnology |
| url | http://hdl.handle.net/10356/16601 |
| work_keys_str_mv | AT linannetteluhe tissueengineeringsmalldiameterbloodvesselsfabricationofporousscaffold |