Adhesion of red blood cells to thrombin activated endothelial cells in plasma like suspensions.
In the cardiovascular system, adhesive interactions between RBCs and ECs are necessary in processes such as inflammation and hemastasis. However, abnormal RBC-EC interactions also occur, causing tissue damage or death. As such, this project was carried out to determine the effects of plasma protein...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/16655 |
| _version_ | 1811680399477178368 |
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| author | Lin, Cheryl Shuyi. |
| author2 | Bjoern Holger Neu |
| author_facet | Bjoern Holger Neu Lin, Cheryl Shuyi. |
| author_sort | Lin, Cheryl Shuyi. |
| collection | NTU |
| description | In the cardiovascular system, adhesive interactions between RBCs and ECs are necessary in processes such as inflammation and hemastasis. However, abnormal RBC-EC
interactions also occur, causing tissue damage or death. As such, this project was carried out to determine the effects of plasma proteins on abnormal RBC-EC interaction in blood, under in-vitro conditions. The effect of plasma proteins on thrombin treated RBCs and ECs were studied using a parallel plate flow chamber system. Variables used in determining the effect of thrombin and plasma proteins on RBC-EC adhesion included: Thrombin concentration, shear stress and Dextran molecular weight.
Experimental results concluded that thrombin induces RBC-EC adhesion. The effect of
increasing thrombin concentration on RBC-EC adhesion followed the trend of a bellshaped
curve, which peaks at thrombin concentration of 0.05 U/ml. Upon introducing the
influence of dextran to thrombin treated RBCs and ECs, strength of RBC-EC adhesion
increases significantly, possibly due to depletion interaction facilitating the adhesion process. |
| first_indexed | 2024-10-01T03:24:26Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16655 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:24:26Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/166552023-03-03T15:33:26Z Adhesion of red blood cells to thrombin activated endothelial cells in plasma like suspensions. Lin, Cheryl Shuyi. Bjoern Holger Neu School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biochemical engineering In the cardiovascular system, adhesive interactions between RBCs and ECs are necessary in processes such as inflammation and hemastasis. However, abnormal RBC-EC interactions also occur, causing tissue damage or death. As such, this project was carried out to determine the effects of plasma proteins on abnormal RBC-EC interaction in blood, under in-vitro conditions. The effect of plasma proteins on thrombin treated RBCs and ECs were studied using a parallel plate flow chamber system. Variables used in determining the effect of thrombin and plasma proteins on RBC-EC adhesion included: Thrombin concentration, shear stress and Dextran molecular weight. Experimental results concluded that thrombin induces RBC-EC adhesion. The effect of increasing thrombin concentration on RBC-EC adhesion followed the trend of a bellshaped curve, which peaks at thrombin concentration of 0.05 U/ml. Upon introducing the influence of dextran to thrombin treated RBCs and ECs, strength of RBC-EC adhesion increases significantly, possibly due to depletion interaction facilitating the adhesion process. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-28T01:30:34Z 2009-05-28T01:30:34Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16655 en Nanyang Technological University 51 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biochemical engineering Lin, Cheryl Shuyi. Adhesion of red blood cells to thrombin activated endothelial cells in plasma like suspensions. |
| title | Adhesion of red blood cells to thrombin activated endothelial cells in plasma like suspensions. |
| title_full | Adhesion of red blood cells to thrombin activated endothelial cells in plasma like suspensions. |
| title_fullStr | Adhesion of red blood cells to thrombin activated endothelial cells in plasma like suspensions. |
| title_full_unstemmed | Adhesion of red blood cells to thrombin activated endothelial cells in plasma like suspensions. |
| title_short | Adhesion of red blood cells to thrombin activated endothelial cells in plasma like suspensions. |
| title_sort | adhesion of red blood cells to thrombin activated endothelial cells in plasma like suspensions |
| topic | DRNTU::Engineering::Chemical engineering::Biochemical engineering |
| url | http://hdl.handle.net/10356/16655 |
| work_keys_str_mv | AT lincherylshuyi adhesionofredbloodcellstothrombinactivatedendothelialcellsinplasmalikesuspensions |