Design and development of bioreactor for smooth muscle cells cultivation
Tissue engineering is an interdisciplinary field that was used to develop biological or hybrid substitutes for implantation into the body for remodeling and regeneration of disease tissue. The focus of this report is to design and fabricate a perfusion bioreactor, which is subsequently used for the...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/10356/16667 |
| _version_ | 1811681766279217152 |
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| author | Tang, Li Juan. |
| author2 | Susanna Leong Su Jan |
| author_facet | Susanna Leong Su Jan Tang, Li Juan. |
| author_sort | Tang, Li Juan. |
| collection | NTU |
| description | Tissue engineering is an interdisciplinary field that was used to develop biological or hybrid substitutes for implantation into the body for remodeling and regeneration of disease tissue. The focus of this report is to design and fabricate a perfusion bioreactor, which is subsequently used for the culturing of smooth muscle cells.
In order to ensure the success of seeding the smooth muscle cells onto the scaffold, research was conducted to ensure that the biomimetic environment provided in the subsequent experiment is suitable for cell culturing and cell proliferation. As such, it was determined that all the experiments involving cell cultures are performed in a 5% CO2 incubator, at 37oC.
The emphasis of this report was to determine the factors required to optimize the cell seeding efficiency using the perfusion bioreactor that was designed. The factors that were studied include the flow configuration, the flow rates and the thickness of scaffold used.
From the experiments conducted, it was concluded that the optimum cell seeding efficiency was achieved using a radial flow configuration, at a flow rate of 1 ml/s to seed the cell culture on a scaffold with 5 mm thickness. |
| first_indexed | 2024-10-01T03:46:09Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/16667 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:46:09Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/166672023-03-03T15:33:57Z Design and development of bioreactor for smooth muscle cells cultivation Tang, Li Juan. Susanna Leong Su Jan School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology Tissue engineering is an interdisciplinary field that was used to develop biological or hybrid substitutes for implantation into the body for remodeling and regeneration of disease tissue. The focus of this report is to design and fabricate a perfusion bioreactor, which is subsequently used for the culturing of smooth muscle cells. In order to ensure the success of seeding the smooth muscle cells onto the scaffold, research was conducted to ensure that the biomimetic environment provided in the subsequent experiment is suitable for cell culturing and cell proliferation. As such, it was determined that all the experiments involving cell cultures are performed in a 5% CO2 incubator, at 37oC. The emphasis of this report was to determine the factors required to optimize the cell seeding efficiency using the perfusion bioreactor that was designed. The factors that were studied include the flow configuration, the flow rates and the thickness of scaffold used. From the experiments conducted, it was concluded that the optimum cell seeding efficiency was achieved using a radial flow configuration, at a flow rate of 1 ml/s to seed the cell culture on a scaffold with 5 mm thickness. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-28T01:48:02Z 2009-05-28T01:48:02Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16667 en Nanyang Technological University 57 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biotechnology Tang, Li Juan. Design and development of bioreactor for smooth muscle cells cultivation |
| title | Design and development of bioreactor for smooth muscle cells cultivation |
| title_full | Design and development of bioreactor for smooth muscle cells cultivation |
| title_fullStr | Design and development of bioreactor for smooth muscle cells cultivation |
| title_full_unstemmed | Design and development of bioreactor for smooth muscle cells cultivation |
| title_short | Design and development of bioreactor for smooth muscle cells cultivation |
| title_sort | design and development of bioreactor for smooth muscle cells cultivation |
| topic | DRNTU::Engineering::Chemical engineering::Biotechnology |
| url | http://hdl.handle.net/10356/16667 |
| work_keys_str_mv | AT tanglijuan designanddevelopmentofbioreactorforsmoothmusclecellscultivation |