Artificial metalloenzymes from bovine β-lactoglobulin and transition metal N-functionalised N-heterocyclic carbene cofactors
Drawing inspiration from natural metalloenzymes such as hydrogenases and nitrogenases and being involved in many biological processes, artificial metalloenzymes involve the assembly of synthetic metal cofactors to a protein host to create biohybrids having tailored catalytic activity. A series of...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2023
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| Online Access: | https://hdl.handle.net/10356/168536 |
| _version_ | 1826113767650361344 |
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| author | Ang, Po Kai |
| author2 | Leung Pak Hing |
| author_facet | Leung Pak Hing Ang, Po Kai |
| author_sort | Ang, Po Kai |
| collection | NTU |
| description | Drawing inspiration from natural metalloenzymes such as hydrogenases and nitrogenases
and being involved in many biological processes, artificial metalloenzymes involve the
assembly of synthetic metal cofactors to a protein host to create biohybrids having tailored
catalytic activity. A series of N-functionalised N-heterocyclic carbene (NHC) metal complexes
featuring long aliphatic carbon chains has been synthesized and their subsequent assembly to
bovine β-lactoglobulin, a protein found in the whey fraction of milk, was investigated. The
catalytic potential of both the NHC-metal cofactors and bioconjugates was studied via the hydroarylation of a pro-fluorescent substrate. |
| first_indexed | 2024-10-01T03:28:37Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/168536 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:28:37Z |
| publishDate | 2023 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1685362023-07-04T01:52:12Z Artificial metalloenzymes from bovine β-lactoglobulin and transition metal N-functionalised N-heterocyclic carbene cofactors Ang, Po Kai Leung Pak Hing School of Chemistry, Chemical Engineering and Biotechnology Sorbonne Université pakhing@ntu.edu.sg Science::Chemistry Drawing inspiration from natural metalloenzymes such as hydrogenases and nitrogenases and being involved in many biological processes, artificial metalloenzymes involve the assembly of synthetic metal cofactors to a protein host to create biohybrids having tailored catalytic activity. A series of N-functionalised N-heterocyclic carbene (NHC) metal complexes featuring long aliphatic carbon chains has been synthesized and their subsequent assembly to bovine β-lactoglobulin, a protein found in the whey fraction of milk, was investigated. The catalytic potential of both the NHC-metal cofactors and bioconjugates was studied via the hydroarylation of a pro-fluorescent substrate. Doctor of Philosophy 2023-06-07T01:52:41Z 2023-06-07T01:52:41Z 2023 Thesis-Doctor of Philosophy Ang, P. K. (2023). Artificial metalloenzymes from bovine β-lactoglobulin and transition metal N-functionalised N-heterocyclic carbene cofactors. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/168536 https://hdl.handle.net/10356/168536 10.32657/10356/168536 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Chemistry Ang, Po Kai Artificial metalloenzymes from bovine β-lactoglobulin and transition metal N-functionalised N-heterocyclic carbene cofactors |
| title | Artificial metalloenzymes from bovine β-lactoglobulin and transition metal N-functionalised N-heterocyclic carbene cofactors |
| title_full | Artificial metalloenzymes from bovine β-lactoglobulin and transition metal N-functionalised N-heterocyclic carbene cofactors |
| title_fullStr | Artificial metalloenzymes from bovine β-lactoglobulin and transition metal N-functionalised N-heterocyclic carbene cofactors |
| title_full_unstemmed | Artificial metalloenzymes from bovine β-lactoglobulin and transition metal N-functionalised N-heterocyclic carbene cofactors |
| title_short | Artificial metalloenzymes from bovine β-lactoglobulin and transition metal N-functionalised N-heterocyclic carbene cofactors |
| title_sort | artificial metalloenzymes from bovine β lactoglobulin and transition metal n functionalised n heterocyclic carbene cofactors |
| topic | Science::Chemistry |
| url | https://hdl.handle.net/10356/168536 |
| work_keys_str_mv | AT angpokai artificialmetalloenzymesfrombovineblactoglobulinandtransitionmetalnfunctionalisednheterocycliccarbenecofactors |