Crystal growth of 3D poly(ε-caprolactone) based bone scaffolds and its effects on the physical properties and cellular interactions
Extrusion additive manufacturing is widely used to fabricate polymer-based 3D bone scaffolds. However, the insight views of crystal growths, scaffold features and eventually cell-scaffold interactions are still unknown. In this work, melt and solvent extrusion additive manufacturing techniques are u...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Online Access: | https://hdl.handle.net/10356/168679 |
| _version_ | 1811692903583449088 |
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| author | Huang, Boyang Wang, Yaxin Vyas, Cian Bartolo, Paulo |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Huang, Boyang Wang, Yaxin Vyas, Cian Bartolo, Paulo |
| author_sort | Huang, Boyang |
| collection | NTU |
| description | Extrusion additive manufacturing is widely used to fabricate polymer-based 3D bone scaffolds. However, the insight views of crystal growths, scaffold features and eventually cell-scaffold interactions are still unknown. In this work, melt and solvent extrusion additive manufacturing techniques are used to produce scaffolds considering highly analogous printing conditions. Results show that the scaffolds produced by these two techniques present distinct physiochemical properties, with melt-printed scaffolds showing stronger mechanical properties and solvent-printed scaffolds showing rougher surface, higher degradation rate, and faster stress relaxation. These differences are attributed to the two different crystal growth kinetics, temperature-induced crystallization (TIC) and strain-induced crystallization (SIC), forming large/integrated spherulite-like and a small/fragmented lamella-like crystal regions respectively. The stiffer substrate of melt-printed scaffolds contributes to higher ratio of nuclear Yes-associated protein (YAP) allocation, favoring cell proliferation and differentiation. Faster relaxation and degradation of solvent-printed scaffolds result in dynamic surface, contributing to an early-stage faster osteogenesis differentiation. |
| first_indexed | 2024-10-01T06:43:11Z |
| format | Journal Article |
| id | ntu-10356/168679 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:43:11Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1686792023-06-14T15:36:56Z Crystal growth of 3D poly(ε-caprolactone) based bone scaffolds and its effects on the physical properties and cellular interactions Huang, Boyang Wang, Yaxin Vyas, Cian Bartolo, Paulo School of Mechanical and Aerospace Engineering Singapore Centre for 3D Printing Engineering::Mechanical engineering Mechanotransduction Melt Printing Extrusion additive manufacturing is widely used to fabricate polymer-based 3D bone scaffolds. However, the insight views of crystal growths, scaffold features and eventually cell-scaffold interactions are still unknown. In this work, melt and solvent extrusion additive manufacturing techniques are used to produce scaffolds considering highly analogous printing conditions. Results show that the scaffolds produced by these two techniques present distinct physiochemical properties, with melt-printed scaffolds showing stronger mechanical properties and solvent-printed scaffolds showing rougher surface, higher degradation rate, and faster stress relaxation. These differences are attributed to the two different crystal growth kinetics, temperature-induced crystallization (TIC) and strain-induced crystallization (SIC), forming large/integrated spherulite-like and a small/fragmented lamella-like crystal regions respectively. The stiffer substrate of melt-printed scaffolds contributes to higher ratio of nuclear Yes-associated protein (YAP) allocation, favoring cell proliferation and differentiation. Faster relaxation and degradation of solvent-printed scaffolds result in dynamic surface, contributing to an early-stage faster osteogenesis differentiation. Published version The authors acknowledge the funding provided by the United Kingdom En-gineering and Physical Sciences Research Council (EPSRC) and the Globa Challenges Research Fund (GCRF) (EP/R01513/1) and Doctoral Prize Fel-lowship (EP/R513131/1). This work was partially supported by the HenryRoyce Institute for Advanced Materials, funded through EPSRC grantsEP/R00661X/1, EP/S019367/1, EP/P025021/1, and EP/P025498/1. 2023-06-14T06:17:17Z 2023-06-14T06:17:17Z 2022 Journal Article Huang, B., Wang, Y., Vyas, C. & Bartolo, P. (2022). Crystal growth of 3D poly(ε-caprolactone) based bone scaffolds and its effects on the physical properties and cellular interactions. Advanced Science, 10(1), 2203183-. https://dx.doi.org/10.1002/advs.202203183 2198-3844 https://hdl.handle.net/10356/168679 10.1002/advs.202203183 36394087 2-s2.0-85142286545 1 10 2203183 en Advanced Science © 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. application/pdf |
| spellingShingle | Engineering::Mechanical engineering Mechanotransduction Melt Printing Huang, Boyang Wang, Yaxin Vyas, Cian Bartolo, Paulo Crystal growth of 3D poly(ε-caprolactone) based bone scaffolds and its effects on the physical properties and cellular interactions |
| title | Crystal growth of 3D poly(ε-caprolactone) based bone scaffolds and its effects on the physical properties and cellular interactions |
| title_full | Crystal growth of 3D poly(ε-caprolactone) based bone scaffolds and its effects on the physical properties and cellular interactions |
| title_fullStr | Crystal growth of 3D poly(ε-caprolactone) based bone scaffolds and its effects on the physical properties and cellular interactions |
| title_full_unstemmed | Crystal growth of 3D poly(ε-caprolactone) based bone scaffolds and its effects on the physical properties and cellular interactions |
| title_short | Crystal growth of 3D poly(ε-caprolactone) based bone scaffolds and its effects on the physical properties and cellular interactions |
| title_sort | crystal growth of 3d poly ε caprolactone based bone scaffolds and its effects on the physical properties and cellular interactions |
| topic | Engineering::Mechanical engineering Mechanotransduction Melt Printing |
| url | https://hdl.handle.net/10356/168679 |
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