Surface ligand influences the Cu nanoclusters as a dual sensing optical probe for localized pH environment and fluoride ion
Functional metal nanomaterials, especially in the nanocluster (NC) size regime, with strong fluorescence, aqueous colloidal stability, and low toxicity, necessitate their application potential in biology and environmental science. Here, we successfully report a simple cost-effective method for red-/...
| Huvudupphovsmän: | , , , , , , |
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| Materialtyp: | Journal Article |
| Språk: | English |
| Publicerad: |
2023
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| Länkar: | https://hdl.handle.net/10356/168803 |
| _version_ | 1826118980289429504 |
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| author | Busi, Kumar Babu Das, Subhalaxmi Palanivel, Mathangi Ghosh, Krishna Kanta Gulyás, Balázs Padmanabhan, Parasuraman Chakrabortty, Sabyasachi |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Busi, Kumar Babu Das, Subhalaxmi Palanivel, Mathangi Ghosh, Krishna Kanta Gulyás, Balázs Padmanabhan, Parasuraman Chakrabortty, Sabyasachi |
| author_sort | Busi, Kumar Babu |
| collection | NTU |
| description | Functional metal nanomaterials, especially in the nanocluster (NC) size regime, with strong fluorescence, aqueous colloidal stability, and low toxicity, necessitate their application potential in biology and environmental science. Here, we successfully report a simple cost-effective method for red-/green-color-emitting protein/amino-acid-mediated Cu NCs in an aqueous medium. As-synthesized Cu NCs were characterized through UV-Vis absorption spectroscopy, fluorescence spectroscopy, time-resolved photoluminescence, dynamic light scattering, zeta potential, transmission electron microscopy and X-ray photoelectron spectroscopy. The optical properties of both Cu NCs responded linearly to the variation in pH in the neutral and alkaline ranges, and a robust pH reversible nature (between pH 7 and 11) was observed that could be extended to rapid, localized pH sensor development. However, a contrasting pH response nature between protein-Cu NCs and amino acid-Cu NCs was recorded. The alteration in protein secondary structure and strong binding nature of the surfactants were suggested to explain this behavior. Furthermore, we investigated their use as an efficient optical probe for fluoride ion detection. The limit of detection for protein-Cu NCs is 6.74 µM, whereas the limit of detection for amino acid-Cu NCs is 4.67 µM. Thus, it is anticipated that ultrasmall Cu NCs will exhibit promise in biological and environmental sensing applications. |
| first_indexed | 2024-10-01T04:52:27Z |
| format | Journal Article |
| id | ntu-10356/168803 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:52:27Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1688032023-06-25T15:37:34Z Surface ligand influences the Cu nanoclusters as a dual sensing optical probe for localized pH environment and fluoride ion Busi, Kumar Babu Das, Subhalaxmi Palanivel, Mathangi Ghosh, Krishna Kanta Gulyás, Balázs Padmanabhan, Parasuraman Chakrabortty, Sabyasachi Lee Kong Chian School of Medicine (LKCMedicine) Cognitive Neuroimaging Centre Science::Medicine Copper Nanoclusters Bovine Serum Albumin Functional metal nanomaterials, especially in the nanocluster (NC) size regime, with strong fluorescence, aqueous colloidal stability, and low toxicity, necessitate their application potential in biology and environmental science. Here, we successfully report a simple cost-effective method for red-/green-color-emitting protein/amino-acid-mediated Cu NCs in an aqueous medium. As-synthesized Cu NCs were characterized through UV-Vis absorption spectroscopy, fluorescence spectroscopy, time-resolved photoluminescence, dynamic light scattering, zeta potential, transmission electron microscopy and X-ray photoelectron spectroscopy. The optical properties of both Cu NCs responded linearly to the variation in pH in the neutral and alkaline ranges, and a robust pH reversible nature (between pH 7 and 11) was observed that could be extended to rapid, localized pH sensor development. However, a contrasting pH response nature between protein-Cu NCs and amino acid-Cu NCs was recorded. The alteration in protein secondary structure and strong binding nature of the surfactants were suggested to explain this behavior. Furthermore, we investigated their use as an efficient optical probe for fluoride ion detection. The limit of detection for protein-Cu NCs is 6.74 µM, whereas the limit of detection for amino acid-Cu NCs is 4.67 µM. Thus, it is anticipated that ultrasmall Cu NCs will exhibit promise in biological and environmental sensing applications. Published version SC thank SRM University, AP research funding (SRMAP/URG/E&PP/2022-23/014) for financial support. 2023-06-19T07:29:52Z 2023-06-19T07:29:52Z 2023 Journal Article Busi, K. B., Das, S., Palanivel, M., Ghosh, K. K., Gulyás, B., Padmanabhan, P. & Chakrabortty, S. (2023). Surface ligand influences the Cu nanoclusters as a dual sensing optical probe for localized pH environment and fluoride ion. Nanomaterials, 13(3), 529-. https://dx.doi.org/10.3390/nano13030529 2079-4991 https://hdl.handle.net/10356/168803 10.3390/nano13030529 36770489 2-s2.0-85147703795 3 13 529 en Nanomaterials © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). application/pdf |
| spellingShingle | Science::Medicine Copper Nanoclusters Bovine Serum Albumin Busi, Kumar Babu Das, Subhalaxmi Palanivel, Mathangi Ghosh, Krishna Kanta Gulyás, Balázs Padmanabhan, Parasuraman Chakrabortty, Sabyasachi Surface ligand influences the Cu nanoclusters as a dual sensing optical probe for localized pH environment and fluoride ion |
| title | Surface ligand influences the Cu nanoclusters as a dual sensing optical probe for localized pH environment and fluoride ion |
| title_full | Surface ligand influences the Cu nanoclusters as a dual sensing optical probe for localized pH environment and fluoride ion |
| title_fullStr | Surface ligand influences the Cu nanoclusters as a dual sensing optical probe for localized pH environment and fluoride ion |
| title_full_unstemmed | Surface ligand influences the Cu nanoclusters as a dual sensing optical probe for localized pH environment and fluoride ion |
| title_short | Surface ligand influences the Cu nanoclusters as a dual sensing optical probe for localized pH environment and fluoride ion |
| title_sort | surface ligand influences the cu nanoclusters as a dual sensing optical probe for localized ph environment and fluoride ion |
| topic | Science::Medicine Copper Nanoclusters Bovine Serum Albumin |
| url | https://hdl.handle.net/10356/168803 |
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