A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities
Precipitation has been studied as an alternative downstream processing step of biopharmaceuticals to replace chromatography. In protein precipitation, previous studies showed precipitation efficiency and product qualities were influenced by the precipitation agents (e.g., salts, organic solvents, po...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/168863 |
| _version_ | 1826129528112545792 |
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| author | Pu, Siyu Hadinoto, Kunn |
| author2 | School of Chemistry, Chemical Engineering and Biotechnology |
| author_facet | School of Chemistry, Chemical Engineering and Biotechnology Pu, Siyu Hadinoto, Kunn |
| author_sort | Pu, Siyu |
| collection | NTU |
| description | Precipitation has been studied as an alternative downstream processing step of biopharmaceuticals to replace chromatography. In protein precipitation, previous studies showed precipitation efficiency and product qualities were influenced by the precipitation agents (e.g., salts, organic solvents, polymers). For bioactive peptides, however, the impacts of precipitation agents had rarely been studied. We previously studied salting-out precipitation of glycopeptide vancomycin (Van) used as the model peptide. The present work compared antisolvent (with acetone) and salting-out precipitations in their precipitation efficiency and product qualities. The phase behavior study showed that heavy precipitates composed of nanoparticles were the predominant products of antisolvent precipitation, in contrast to crystalline microparticles produced by salting-out. At their respective optimal conditions (e.g., pH, Van concentration), batch antisolvent precipitation had significantly higher yield than salting-out. Other than faster dissolution of antisolvent precipitates attributed to their smaller size, both precipitates exhibited comparable purity, peptide secondary structures, thermal stability, and antimicrobial activity. |
| first_indexed | 2024-10-01T07:42:04Z |
| format | Journal Article |
| id | ntu-10356/168863 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:42:04Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | ntu-10356/1688632023-06-20T08:48:11Z A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities Pu, Siyu Hadinoto, Kunn School of Chemistry, Chemical Engineering and Biotechnology Engineering::Chemical engineering Protein Precipitation Peptide Purification Precipitation has been studied as an alternative downstream processing step of biopharmaceuticals to replace chromatography. In protein precipitation, previous studies showed precipitation efficiency and product qualities were influenced by the precipitation agents (e.g., salts, organic solvents, polymers). For bioactive peptides, however, the impacts of precipitation agents had rarely been studied. We previously studied salting-out precipitation of glycopeptide vancomycin (Van) used as the model peptide. The present work compared antisolvent (with acetone) and salting-out precipitations in their precipitation efficiency and product qualities. The phase behavior study showed that heavy precipitates composed of nanoparticles were the predominant products of antisolvent precipitation, in contrast to crystalline microparticles produced by salting-out. At their respective optimal conditions (e.g., pH, Van concentration), batch antisolvent precipitation had significantly higher yield than salting-out. Other than faster dissolution of antisolvent precipitates attributed to their smaller size, both precipitates exhibited comparable purity, peptide secondary structures, thermal stability, and antimicrobial activity. Ministry of Education (MOE) The authors would like to acknowledge the research funding from Ministry of Education Singapore (Grant No. AcRF RG82/20). 2023-06-20T08:48:11Z 2023-06-20T08:48:11Z 2023 Journal Article Pu, S. & Hadinoto, K. (2023). A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities. Powder Technology, 415, 118181-. https://dx.doi.org/10.1016/j.powtec.2022.118181 0032-5910 https://hdl.handle.net/10356/168863 10.1016/j.powtec.2022.118181 2-s2.0-85144613034 415 118181 en RG82/20 Powder Technology © 2022 Elsevier B.V. All rights reserved. |
| spellingShingle | Engineering::Chemical engineering Protein Precipitation Peptide Purification Pu, Siyu Hadinoto, Kunn A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities |
| title | A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities |
| title_full | A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities |
| title_fullStr | A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities |
| title_full_unstemmed | A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities |
| title_short | A comparative study of antisolvent versus salting-out precipitations of glycopeptide vancomycin: precipitation efficiency and product qualities |
| title_sort | comparative study of antisolvent versus salting out precipitations of glycopeptide vancomycin precipitation efficiency and product qualities |
| topic | Engineering::Chemical engineering Protein Precipitation Peptide Purification |
| url | https://hdl.handle.net/10356/168863 |
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